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Mg(2+)-dependent conformational changes and product release during DNA-catalyzed RNA ligation monitored by Bimane fluorescence
Among the deoxyribozymes catalyzing the ligation of two RNA substrates, 7S11 generates a branched RNA containing a 2′,5′-linkage. We have attached the small fluorogenic probe Bimane to the triphosphate terminated RNA substrate and utilized emission intensity and anisotropy to follow structural rearr...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Oxford University Press
2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4288166/ https://www.ncbi.nlm.nih.gov/pubmed/25505142 http://dx.doi.org/10.1093/nar/gku1268 |