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Active destabilization of base pairs by a DNA glycosylase wedge initiates damage recognition
Formamidopyrimidine-DNA glycosylase (Fpg) excises 8-oxoguanine (oxoG) from DNA but ignores normal guanine. We combined molecular dynamics simulation and stopped-flow kinetics with fluorescence detection to track the events in the recognition of oxoG by Fpg and its mutants with a key phenylalanine re...
Autores principales: | Kuznetsov, Nikita A., Bergonzo, Christina, Campbell, Arthur J., Li, Haoquan, Mechetin, Grigory V., de los Santos, Carlos, Grollman, Arthur P., Fedorova, Olga S., Zharkov, Dmitry O., Simmerling, Carlos |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4288190/ https://www.ncbi.nlm.nih.gov/pubmed/25520195 http://dx.doi.org/10.1093/nar/gku1300 |
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