Nic1 Inactivation Enables Stable Isotope Labeling with (13)C(6)(15)N(4)-Arginine in Schizosaccharomyces pombe

Stable Isotope Labeling by Amino Acids (SILAC) is a commonly used method in quantitative proteomics. Because of compatibility with trypsin digestion, arginine and lysine are the most widely used amino acids for SILAC labeling. We observed that Schizosaccharomyces pombe (fission yeast) cannot be labe...

Descripción completa

Detalles Bibliográficos
Autores principales: Carpy, Alejandro, Patel, Avinash, Tay, Ye Dee, Hagan, Iain M., Macek, Boris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Biochemistry and Molecular Biology 2015
Materias:
Technological Innovation and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4288259/
https://www.ncbi.nlm.nih.gov/pubmed/25368411
http://dx.doi.org/10.1074/mcp.O114.045302

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4288259/
https://www.ncbi.nlm.nih.gov/pubmed/25368411
http://dx.doi.org/10.1074/mcp.O114.045302

Ejemplares similares