Cargando…

Nic1 Inactivation Enables Stable Isotope Labeling with (13)C(6)(15)N(4)-Arginine in Schizosaccharomyces pombe

Stable Isotope Labeling by Amino Acids (SILAC) is a commonly used method in quantitative proteomics. Because of compatibility with trypsin digestion, arginine and lysine are the most widely used amino acids for SILAC labeling. We observed that Schizosaccharomyces pombe (fission yeast) cannot be labe...

Descripción completa

Detalles Bibliográficos
Autores principales:	Carpy, Alejandro, Patel, Avinash, Tay, Ye Dee, Hagan, Iain M., Macek, Boris
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The American Society for Biochemistry and Molecular Biology 2015
Materias:	Technological Innovation and Resources
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4288259/ https://www.ncbi.nlm.nih.gov/pubmed/25368411 http://dx.doi.org/10.1074/mcp.O114.045302

Ejemplares similares

Extensive Mass Spectrometry-based Analysis of the Fission Yeast Proteome: THE SCHIZOSACCHAROMYCES POMBE PeptideAtlas*
por: Gunaratne, Jayantha, et al.
Publicado: (2013)

Stable Isotope Dynamic Labeling of Secretomes (SIDLS) Identifies Authentic Secretory Proteins Released by Cancer and Stromal Cells
por: Hammond, Dean E., et al.
Publicado: (2018)

An Enhanced In Vivo Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Model for Quantification of Drug Metabolism Enzymes
por: MacLeod, A. Kenneth, et al.
Publicado: (2015)

Stable Isotope Labeling of Amino Acids in Flies (SILAF) Reveals Differential Phosphorylation of Mitochondrial Proteins Upon Loss of OXPHOS Subunits
por: Schober, Florian A., et al.
Publicado: (2021)

A Method for Large-scale Identification of Protein Arginine Methylation
por: Uhlmann, Thomas, et al.
Publicado: (2012)

Quantitative Tracking of Isotope Flows in Proteomes of Microbial Communities
por: Pan, Chongle, et al.
Publicado: (2011)

Quantitative Cross-linking/Mass Spectrometry Using Isotope-labeled Cross-linkers and MaxQuant
por: Chen, Zhuo A., et al.
Publicado: (2016)

Extending the Schizosaccharomyces pombe Molecular Genetic Toolbox
por: Fennessy, Dorota, et al.
Publicado: (2014)

Fission stories: using PomBase to understand Schizosaccharomyces pombe biology
por: Harris, Midori A, et al.
Publicado: (2021)

Initial Quantitative Proteomic Map of 28 Mouse Tissues Using the SILAC Mouse
por: Geiger, Tamar, et al.
Publicado: (2013)

ProMetheusDB: An In-Depth Analysis of the High-Quality Human Methyl-proteome
por: Massignani, Enrico, et al.
Publicado: (2022)

pyQms enables universal and accurate quantification of mass spectrometry data
por: Leufken, Johannes, et al.
Publicado: (2017)

Protein Aggregation Capture on Microparticles Enables Multipurpose Proteomics Sample Preparation
por: Batth, Tanveer S., et al.
Publicado: (2019)

MaxQuant.Live Enables Global Targeting of More Than 25,000 Peptides
por: Wichmann, Christoph, et al.
Publicado: (2019)

IonQuant Enables Accurate and Sensitive Label-Free Quantification With FDR-Controlled Match-Between-Runs
por: Yu, Fengchao, et al.
Publicado: (2021)

A New Parallel High-Pressure Packing System Enables Rapid Multiplexed Production of Capillary Columns
por: Müller-Reif, Johannes B., et al.
Publicado: (2021)

Enabling Photoactivated Cross-Linking Mass Spectrometric Analysis of Protein Complexes by Novel MS-Cleavable Cross-Linkers
por: Gutierrez, Craig, et al.
Publicado: (2021)

Open Database Searching Enables the Identification and Comparison of Bacterial Glycoproteomes without Defining Glycan Compositions Prior to Searching
por: Ahmad Izaham, Ameera Raudah, et al.
Publicado: (2020)

Structural Analysis of PTM Hotspots (SAPH-ire) – A Quantitative Informatics Method Enabling the Discovery of Novel Regulatory Elements in Protein Families
por: Dewhurst, Henry M., et al.
Publicado: (2015)

The telomeric transcriptome of Schizosaccharomyces pombe
por: Bah, Amadou, et al.
Publicado: (2012)

Response to sulfur in Schizosaccharomyces pombe
por: Ohtsuka, Hokuto, et al.
Publicado: (2021)

In Vivo Analysis of Proteomes and Interactomes Using Parallel Affinity Capture (iPAC) Coupled to Mass Spectrometry
por: Rees, Johanna S., et al.
Publicado: (2011)

Inferring Physical Protein Contacts from Large-Scale Purification Data of Protein Complexes
por: Schelhorn, Sven-Eric, et al.
Publicado: (2011)

Published and Perished? The Influence of the Searched Protein Database on the Long-Term Storage of Proteomics Data
por: Griss, Johannes, et al.
Publicado: (2011)

Spectrum-to-Spectrum Searching Using a Proteome-wide Spectral Library
por: Yen, Chia-Yu, et al.
Publicado: (2011)

Deep and Highly Sensitive Proteome Coverage by LC-MS/MS Without Prefractionation
por: Thakur, Suman S., et al.
Publicado: (2011)

PEAKS DB: De Novo Sequencing Assisted Database Search for Sensitive and Accurate Peptide Identification
por: Zhang, Jing, et al.
Publicado: (2012)

Antibody Colocalization Microarray: A Scalable Technology for Multiplex Protein Analysis in Complex Samples
por: Pla-Roca, M., et al.
Publicado: (2012)

TraML—A Standard Format for Exchange of Selected Reaction Monitoring Transition Lists
por: Deutsch, Eric W., et al.
Publicado: (2012)

Proteomic Analysis and Discovery Using Affinity Proteomics and Mass Spectrometry
por: Olsson, Niclas, et al.
Publicado: (2011)

High Throughput Isolation and Glycosylation Analysis of IgG–Variability and Heritability of the IgG Glycome in Three Isolated Human Populations
por: Pučić, Maja, et al.
Publicado: (2011)

Mass Spectrometry-based Proteomics Using Q Exactive, a High-performance Benchtop Quadrupole Orbitrap Mass Spectrometer
por: Michalski, Annette, et al.
Publicado: (2011)

Signaling Network State Predicts Twist-Mediated Effects on Breast Cell Migration Across Diverse Growth Factor Contexts
por: Kim, Hyung-Do, et al.
Publicado: (2011)

Enhanced Peptide Identification by Electron Transfer Dissociation Using an Improved Mascot Percolator
por: Wright, James C., et al.
Publicado: (2012)

PaxDb, a Database of Protein Abundance Averages Across All Three Domains of Life
por: Wang, M., et al.
Publicado: (2012)

Index-ion Triggered MS2 Ion Quantification: A Novel Proteomics Approach for Reproducible Detection and Quantification of Targeted Proteins in Complex Mixtures
por: Yan, Wei, et al.
Publicado: (2011)

Overcoming Species Boundaries in Peptide Identification with Bayesian Information Criterion-driven Error-tolerant Peptide Search (BICEPS)
por: Renard, Bernhard Y., et al.
Publicado: (2012)

The mzIdentML Data Standard for Mass Spectrometry-Based Proteomics Results
por: Jones, Andrew R., et al.
Publicado: (2012)

Platform-independent and Label-free Quantitation of Proteomic Data Using MS1 Extracted Ion Chromatograms in Skyline: APPLICATION TO PROTEIN ACETYLATION AND PHOSPHORYLATION
por: Schilling, Birgit, et al.
Publicado: (2012)

Matrix Assisted Ionization Vacuum (MAIV), a New Ionization Method for Biological Materials Analysis Using Mass Spectrometry
por: Inutan, Ellen D., et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_5aqmsba2uqi7a3ue8serrhjhno