RAB8A a new biomarker for endometrial cancer?

BACKGROUND: We aimed to find different proteins between dendritic cells (DCs) and tumor antigen-pulsed DCs to help find a new biomarker for endometrial cancer (EC). METHODS: Mononuclear cells were isolated from cord blood and induced to DCs by cytokines. A comparative proteomic analysis was performe...

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Detalles Bibliográficos
Autores principales: Bie, Yachun, Zhang, Zhenyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289371/
https://www.ncbi.nlm.nih.gov/pubmed/25477298
http://dx.doi.org/10.1186/1477-7819-12-371
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author Bie, Yachun
Zhang, Zhenyu
author_facet Bie, Yachun
Zhang, Zhenyu
author_sort Bie, Yachun
collection PubMed
description BACKGROUND: We aimed to find different proteins between dendritic cells (DCs) and tumor antigen-pulsed DCs to help find a new biomarker for endometrial cancer (EC). METHODS: Mononuclear cells were isolated from cord blood and induced to DCs by cytokines. A comparative proteomic analysis was performed on DCs and tumor lysate-pulsed DCs by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Differential proteins were identified by Western blot analysis. RESULTS: The expression of Ras-related protein Rab-8A (RAB8A) was found to be different in the in two kinds of cells. This phenomenon was also proven in endometrial cancer tissues. CONCLUSIONS: RAB8A might be a new biomarker for endometrial cancer. Using LC-MS/MS to perform a comparative proteomic analysis about DCs and tumor lysate-pulsed DCs may help us to find new biomarker of cancer.
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spelling pubmed-42893712015-01-11 RAB8A a new biomarker for endometrial cancer? Bie, Yachun Zhang, Zhenyu World J Surg Oncol Research BACKGROUND: We aimed to find different proteins between dendritic cells (DCs) and tumor antigen-pulsed DCs to help find a new biomarker for endometrial cancer (EC). METHODS: Mononuclear cells were isolated from cord blood and induced to DCs by cytokines. A comparative proteomic analysis was performed on DCs and tumor lysate-pulsed DCs by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Differential proteins were identified by Western blot analysis. RESULTS: The expression of Ras-related protein Rab-8A (RAB8A) was found to be different in the in two kinds of cells. This phenomenon was also proven in endometrial cancer tissues. CONCLUSIONS: RAB8A might be a new biomarker for endometrial cancer. Using LC-MS/MS to perform a comparative proteomic analysis about DCs and tumor lysate-pulsed DCs may help us to find new biomarker of cancer. BioMed Central 2014-12-04 /pmc/articles/PMC4289371/ /pubmed/25477298 http://dx.doi.org/10.1186/1477-7819-12-371 Text en © Bie and Zhang; licensee BioMed Central. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Bie, Yachun
Zhang, Zhenyu
RAB8A a new biomarker for endometrial cancer?
title RAB8A a new biomarker for endometrial cancer?
title_full RAB8A a new biomarker for endometrial cancer?
title_fullStr RAB8A a new biomarker for endometrial cancer?
title_full_unstemmed RAB8A a new biomarker for endometrial cancer?
title_short RAB8A a new biomarker for endometrial cancer?
title_sort rab8a a new biomarker for endometrial cancer?
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289371/
https://www.ncbi.nlm.nih.gov/pubmed/25477298
http://dx.doi.org/10.1186/1477-7819-12-371
work_keys_str_mv AT bieyachun rab8aanewbiomarkerforendometrialcancer
AT zhangzhenyu rab8aanewbiomarkerforendometrialcancer

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