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Short Hairpin Ribonucleic Acid Constructs Targeting Insulin-like Growth Factor Binding Protein-3 Reversed Decreased Testosterone Concentrations in Diabetic Rats
BACKGROUND: The aim of this study was to determine if shRNA constructs targeting insulin-like growth factor binding protein-3 can rehabilitate decreased serum testosterone concentrations in streptozotocin-induced diabetic rats. MATERIAL/METHODS: After 12 weeks of intracavernous administration of IGF...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289482/ https://www.ncbi.nlm.nih.gov/pubmed/25582342 http://dx.doi.org/10.12659/MSM.891382 |
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author | Zhou, Zhang-Yan Fei-Li, Cheng, Shao-Ping Huang, Hui Peng, Bi-Wen Wang, Jing Liu, Chang-Mao Xing, Cheng Sun, Ya-Ling Bsoul, Najeeb Pan, Hui Yi, Cun-Jian Liu, Rong-Hua Zhong, Guang-Jun |
author_facet | Zhou, Zhang-Yan Fei-Li, Cheng, Shao-Ping Huang, Hui Peng, Bi-Wen Wang, Jing Liu, Chang-Mao Xing, Cheng Sun, Ya-Ling Bsoul, Najeeb Pan, Hui Yi, Cun-Jian Liu, Rong-Hua Zhong, Guang-Jun |
author_sort | Zhou, Zhang-Yan |
collection | PubMed |
description | BACKGROUND: The aim of this study was to determine if shRNA constructs targeting insulin-like growth factor binding protein-3 can rehabilitate decreased serum testosterone concentrations in streptozotocin-induced diabetic rats. MATERIAL/METHODS: After 12 weeks of intracavernous administration of IGFBP-3 shRNA, intracavernous pressure responses to electrical stimulation of cavernous nerves were evaluated. The expression of IGFBP-3 at mRNA and protein levels was detected by quantitative real-time PCR analysis and Western blot, respectively. The concentrations of serum testosterone and cavernous cyclic guanosine monophosphate were detected by enzyme-linked immunosorbent assay. RESULTS: After 12 weeks of intracavernous administration of IGFBP-3 shRNA, the cavernosal pressure was significantly increased in response to the cavernous nerves stimulation compared to the diabetic control group (p<0.01). Cavernous IGFBP-3 expression at both mRNA and protein levels was significantly inhibited. Both serum testosterone and cavernous cyclic guanosine monophosphate concentrations were significantly increased in the IGFBP-3 shRNA treatment group compared to the diabetic control group (p<0.01). CONCLUSIONS: These results suggest that IGFBP-3 shRNA may rehabilitate erectile function via increases of concentrations of serum testosterone and cavernous cyclic guanosine monophosphate in streptozotocin-induced diabetic rats. |
format | Online Article Text |
id | pubmed-4289482 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-42894822015-01-12 Short Hairpin Ribonucleic Acid Constructs Targeting Insulin-like Growth Factor Binding Protein-3 Reversed Decreased Testosterone Concentrations in Diabetic Rats Zhou, Zhang-Yan Fei-Li, Cheng, Shao-Ping Huang, Hui Peng, Bi-Wen Wang, Jing Liu, Chang-Mao Xing, Cheng Sun, Ya-Ling Bsoul, Najeeb Pan, Hui Yi, Cun-Jian Liu, Rong-Hua Zhong, Guang-Jun Med Sci Monit Animal Study BACKGROUND: The aim of this study was to determine if shRNA constructs targeting insulin-like growth factor binding protein-3 can rehabilitate decreased serum testosterone concentrations in streptozotocin-induced diabetic rats. MATERIAL/METHODS: After 12 weeks of intracavernous administration of IGFBP-3 shRNA, intracavernous pressure responses to electrical stimulation of cavernous nerves were evaluated. The expression of IGFBP-3 at mRNA and protein levels was detected by quantitative real-time PCR analysis and Western blot, respectively. The concentrations of serum testosterone and cavernous cyclic guanosine monophosphate were detected by enzyme-linked immunosorbent assay. RESULTS: After 12 weeks of intracavernous administration of IGFBP-3 shRNA, the cavernosal pressure was significantly increased in response to the cavernous nerves stimulation compared to the diabetic control group (p<0.01). Cavernous IGFBP-3 expression at both mRNA and protein levels was significantly inhibited. Both serum testosterone and cavernous cyclic guanosine monophosphate concentrations were significantly increased in the IGFBP-3 shRNA treatment group compared to the diabetic control group (p<0.01). CONCLUSIONS: These results suggest that IGFBP-3 shRNA may rehabilitate erectile function via increases of concentrations of serum testosterone and cavernous cyclic guanosine monophosphate in streptozotocin-induced diabetic rats. International Scientific Literature, Inc. 2015-01-08 /pmc/articles/PMC4289482/ /pubmed/25582342 http://dx.doi.org/10.12659/MSM.891382 Text en © Med Sci Monit, 2015 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License |
spellingShingle | Animal Study Zhou, Zhang-Yan Fei-Li, Cheng, Shao-Ping Huang, Hui Peng, Bi-Wen Wang, Jing Liu, Chang-Mao Xing, Cheng Sun, Ya-Ling Bsoul, Najeeb Pan, Hui Yi, Cun-Jian Liu, Rong-Hua Zhong, Guang-Jun Short Hairpin Ribonucleic Acid Constructs Targeting Insulin-like Growth Factor Binding Protein-3 Reversed Decreased Testosterone Concentrations in Diabetic Rats |
title | Short Hairpin Ribonucleic Acid Constructs Targeting Insulin-like Growth Factor Binding Protein-3 Reversed Decreased Testosterone Concentrations in Diabetic Rats |
title_full | Short Hairpin Ribonucleic Acid Constructs Targeting Insulin-like Growth Factor Binding Protein-3 Reversed Decreased Testosterone Concentrations in Diabetic Rats |
title_fullStr | Short Hairpin Ribonucleic Acid Constructs Targeting Insulin-like Growth Factor Binding Protein-3 Reversed Decreased Testosterone Concentrations in Diabetic Rats |
title_full_unstemmed | Short Hairpin Ribonucleic Acid Constructs Targeting Insulin-like Growth Factor Binding Protein-3 Reversed Decreased Testosterone Concentrations in Diabetic Rats |
title_short | Short Hairpin Ribonucleic Acid Constructs Targeting Insulin-like Growth Factor Binding Protein-3 Reversed Decreased Testosterone Concentrations in Diabetic Rats |
title_sort | short hairpin ribonucleic acid constructs targeting insulin-like growth factor binding protein-3 reversed decreased testosterone concentrations in diabetic rats |
topic | Animal Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289482/ https://www.ncbi.nlm.nih.gov/pubmed/25582342 http://dx.doi.org/10.12659/MSM.891382 |
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