Cargando…
Potentially Pathogenic Free-Living Amoebae in Contact Lenses of the Asymptomatic Contact Lens Wearers
BACKGROUND: Free-living amoebae (FLA) including Acanthamoeba spp. and Hartmannella spp. are the causative agents of serious corneal infection especially within contact lens wearers. Thus contact lenses and their storage case could be a suitable niche for potentially pathogenic amoebae. The main obje...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289873/ https://www.ncbi.nlm.nih.gov/pubmed/25642255 |
_version_ | 1782352156512223232 |
---|---|
author | NIYYATI, Maryam RAHIMI, Firoozeh LASEJERDI, Zohreh REZAEIAN, Mostafa |
author_facet | NIYYATI, Maryam RAHIMI, Firoozeh LASEJERDI, Zohreh REZAEIAN, Mostafa |
author_sort | NIYYATI, Maryam |
collection | PubMed |
description | BACKGROUND: Free-living amoebae (FLA) including Acanthamoeba spp. and Hartmannella spp. are the causative agents of serious corneal infection especially within contact lens wearers. Thus contact lenses and their storage case could be a suitable niche for potentially pathogenic amoebae. The main objective of the present study was to evaluate the contamination of contact lenses to free living amoebae using morphological and sequencing based methods. METHODS: Overall, 90 volunteers provided their contact lenses. All volunteers wore soft contact lenses. Both lenses were cultured in the same plate. Forty-eight of the volunteers were medical and dentistry student and 42 were ophthalmology attendees of hospitals in Tehran, Iran. All of the samples were inoculated to non-nutrient medium and monitored daily for the outgrowth of the amoebae. PCR and sequencing were performed using various primer pairs. RESULTS: Of the 90 volunteers, 9 (10%) were positive for free-living amoebae outgrowth. Morphological analysis revealed that 3 isolates were belonged to Hartmannella genus according to small round cysts and 6 isolates were belonged to Acanthamoeba genus based on the star shape of endocysts. Sequencing revealed that Acanthamoeba belonged to T4, T3 and T5 genotype. Hartmannella were also belonged to vermiformis species. DISCUSSION: The presence of potentially pathogenic free living amoebae including Acanthamoeba and Hartmannella could be a high risk for people using soft contact lenses. These results revealed that improved clarification and professional recommendations for contact lens wearers is of utmost importance. |
format | Online Article Text |
id | pubmed-4289873 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-42898732015-01-30 Potentially Pathogenic Free-Living Amoebae in Contact Lenses of the Asymptomatic Contact Lens Wearers NIYYATI, Maryam RAHIMI, Firoozeh LASEJERDI, Zohreh REZAEIAN, Mostafa Iran J Parasitol Original Article BACKGROUND: Free-living amoebae (FLA) including Acanthamoeba spp. and Hartmannella spp. are the causative agents of serious corneal infection especially within contact lens wearers. Thus contact lenses and their storage case could be a suitable niche for potentially pathogenic amoebae. The main objective of the present study was to evaluate the contamination of contact lenses to free living amoebae using morphological and sequencing based methods. METHODS: Overall, 90 volunteers provided their contact lenses. All volunteers wore soft contact lenses. Both lenses were cultured in the same plate. Forty-eight of the volunteers were medical and dentistry student and 42 were ophthalmology attendees of hospitals in Tehran, Iran. All of the samples were inoculated to non-nutrient medium and monitored daily for the outgrowth of the amoebae. PCR and sequencing were performed using various primer pairs. RESULTS: Of the 90 volunteers, 9 (10%) were positive for free-living amoebae outgrowth. Morphological analysis revealed that 3 isolates were belonged to Hartmannella genus according to small round cysts and 6 isolates were belonged to Acanthamoeba genus based on the star shape of endocysts. Sequencing revealed that Acanthamoeba belonged to T4, T3 and T5 genotype. Hartmannella were also belonged to vermiformis species. DISCUSSION: The presence of potentially pathogenic free living amoebae including Acanthamoeba and Hartmannella could be a high risk for people using soft contact lenses. These results revealed that improved clarification and professional recommendations for contact lens wearers is of utmost importance. Tehran University of Medical Sciences 2014-03 /pmc/articles/PMC4289873/ /pubmed/25642255 Text en Copyright: © Iranian Journal of Parasitology & Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article NIYYATI, Maryam RAHIMI, Firoozeh LASEJERDI, Zohreh REZAEIAN, Mostafa Potentially Pathogenic Free-Living Amoebae in Contact Lenses of the Asymptomatic Contact Lens Wearers |
title | Potentially Pathogenic Free-Living Amoebae in Contact Lenses of the Asymptomatic Contact Lens Wearers |
title_full | Potentially Pathogenic Free-Living Amoebae in Contact Lenses of the Asymptomatic Contact Lens Wearers |
title_fullStr | Potentially Pathogenic Free-Living Amoebae in Contact Lenses of the Asymptomatic Contact Lens Wearers |
title_full_unstemmed | Potentially Pathogenic Free-Living Amoebae in Contact Lenses of the Asymptomatic Contact Lens Wearers |
title_short | Potentially Pathogenic Free-Living Amoebae in Contact Lenses of the Asymptomatic Contact Lens Wearers |
title_sort | potentially pathogenic free-living amoebae in contact lenses of the asymptomatic contact lens wearers |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289873/ https://www.ncbi.nlm.nih.gov/pubmed/25642255 |
work_keys_str_mv | AT niyyatimaryam potentiallypathogenicfreelivingamoebaeincontactlensesoftheasymptomaticcontactlenswearers AT rahimifiroozeh potentiallypathogenicfreelivingamoebaeincontactlensesoftheasymptomaticcontactlenswearers AT lasejerdizohreh potentiallypathogenicfreelivingamoebaeincontactlensesoftheasymptomaticcontactlenswearers AT rezaeianmostafa potentiallypathogenicfreelivingamoebaeincontactlensesoftheasymptomaticcontactlenswearers |