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Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran
BACKGROUND: There are some genetic differences in Blastocystis that show the existence of species or genotypes. One of these genes that help in identifying Blastocystis is SSUrRNA. The aim of this study was assessment of genetic diversity of Blastocystis by PCR with seven pairs of STS primers. METHO...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289879/ https://www.ncbi.nlm.nih.gov/pubmed/25642259 |
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author | BADPARVA, Ebrahim SADRAEE, Javid KHEIRANDISH, Farnaz FROUZANDEH, Mehdi |
author_facet | BADPARVA, Ebrahim SADRAEE, Javid KHEIRANDISH, Farnaz FROUZANDEH, Mehdi |
author_sort | BADPARVA, Ebrahim |
collection | PubMed |
description | BACKGROUND: There are some genetic differences in Blastocystis that show the existence of species or genotypes. One of these genes that help in identifying Blastocystis is SSUrRNA. The aim of this study was assessment of genetic diversity of Blastocystis by PCR with seven pairs of STS primers. METHODS: This study was done on 511 stool samples collected from patients referred to the health care centers of Khorramabad, Central Iran, in 2012. Genomic DNA was extracted and in order to determine the Blastocystis subtype in contaminated samples, seven pairs of primers STS (subtype specific sequence-tagged site) were used. RESULTS: Out of 511 samples, 33 (6.5%) samples were infected with Blastocystis. Subtype (ST) of 30 samples was identified and three subtypes 2, 3 and 4 were determined. Mix infection was reported 10% which 3.33% of the infection was for the mixture of ST 3 and ST5 and 6.67% was for the mixture of ST 2 and ST 3. CONCLUSION: The predominant subtype was ST3 that is the main human subtype. The dominance of ST2 and 5 are important in this study. This superiority has been reported in some of the studies in ST 2 which is different from the studies in other countries, because they have announced priorities of the ST1 and ST6 after ST3. |
format | Online Article Text |
id | pubmed-4289879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-42898792015-01-30 Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran BADPARVA, Ebrahim SADRAEE, Javid KHEIRANDISH, Farnaz FROUZANDEH, Mehdi Iran J Parasitol Original Article BACKGROUND: There are some genetic differences in Blastocystis that show the existence of species or genotypes. One of these genes that help in identifying Blastocystis is SSUrRNA. The aim of this study was assessment of genetic diversity of Blastocystis by PCR with seven pairs of STS primers. METHODS: This study was done on 511 stool samples collected from patients referred to the health care centers of Khorramabad, Central Iran, in 2012. Genomic DNA was extracted and in order to determine the Blastocystis subtype in contaminated samples, seven pairs of primers STS (subtype specific sequence-tagged site) were used. RESULTS: Out of 511 samples, 33 (6.5%) samples were infected with Blastocystis. Subtype (ST) of 30 samples was identified and three subtypes 2, 3 and 4 were determined. Mix infection was reported 10% which 3.33% of the infection was for the mixture of ST 3 and ST5 and 6.67% was for the mixture of ST 2 and ST 3. CONCLUSION: The predominant subtype was ST3 that is the main human subtype. The dominance of ST2 and 5 are important in this study. This superiority has been reported in some of the studies in ST 2 which is different from the studies in other countries, because they have announced priorities of the ST1 and ST6 after ST3. Tehran University of Medical Sciences 2014-03 /pmc/articles/PMC4289879/ /pubmed/25642259 Text en Copyright: © Iranian Journal of Parasitology & Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article BADPARVA, Ebrahim SADRAEE, Javid KHEIRANDISH, Farnaz FROUZANDEH, Mehdi Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran |
title | Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran |
title_full | Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran |
title_fullStr | Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran |
title_full_unstemmed | Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran |
title_short | Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran |
title_sort | genetic diversity of human blastocystis isolates in khorramabad, central iran |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289879/ https://www.ncbi.nlm.nih.gov/pubmed/25642259 |
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