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Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran

BACKGROUND: There are some genetic differences in Blastocystis that show the existence of species or genotypes. One of these genes that help in identifying Blastocystis is SSUrRNA. The aim of this study was assessment of genetic diversity of Blastocystis by PCR with seven pairs of STS primers. METHO...

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Autores principales: BADPARVA, Ebrahim, SADRAEE, Javid, KHEIRANDISH, Farnaz, FROUZANDEH, Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289879/
https://www.ncbi.nlm.nih.gov/pubmed/25642259
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author BADPARVA, Ebrahim
SADRAEE, Javid
KHEIRANDISH, Farnaz
FROUZANDEH, Mehdi
author_facet BADPARVA, Ebrahim
SADRAEE, Javid
KHEIRANDISH, Farnaz
FROUZANDEH, Mehdi
author_sort BADPARVA, Ebrahim
collection PubMed
description BACKGROUND: There are some genetic differences in Blastocystis that show the existence of species or genotypes. One of these genes that help in identifying Blastocystis is SSUrRNA. The aim of this study was assessment of genetic diversity of Blastocystis by PCR with seven pairs of STS primers. METHODS: This study was done on 511 stool samples collected from patients referred to the health care centers of Khorramabad, Central Iran, in 2012. Genomic DNA was extracted and in order to determine the Blastocystis subtype in contaminated samples, seven pairs of primers STS (subtype specific sequence-tagged site) were used. RESULTS: Out of 511 samples, 33 (6.5%) samples were infected with Blastocystis. Subtype (ST) of 30 samples was identified and three subtypes 2, 3 and 4 were determined. Mix infection was reported 10% which 3.33% of the infection was for the mixture of ST 3 and ST5 and 6.67% was for the mixture of ST 2 and ST 3. CONCLUSION: The predominant subtype was ST3 that is the main human subtype. The dominance of ST2 and 5 are important in this study. This superiority has been reported in some of the studies in ST 2 which is different from the studies in other countries, because they have announced priorities of the ST1 and ST6 after ST3.
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spelling pubmed-42898792015-01-30 Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran BADPARVA, Ebrahim SADRAEE, Javid KHEIRANDISH, Farnaz FROUZANDEH, Mehdi Iran J Parasitol Original Article BACKGROUND: There are some genetic differences in Blastocystis that show the existence of species or genotypes. One of these genes that help in identifying Blastocystis is SSUrRNA. The aim of this study was assessment of genetic diversity of Blastocystis by PCR with seven pairs of STS primers. METHODS: This study was done on 511 stool samples collected from patients referred to the health care centers of Khorramabad, Central Iran, in 2012. Genomic DNA was extracted and in order to determine the Blastocystis subtype in contaminated samples, seven pairs of primers STS (subtype specific sequence-tagged site) were used. RESULTS: Out of 511 samples, 33 (6.5%) samples were infected with Blastocystis. Subtype (ST) of 30 samples was identified and three subtypes 2, 3 and 4 were determined. Mix infection was reported 10% which 3.33% of the infection was for the mixture of ST 3 and ST5 and 6.67% was for the mixture of ST 2 and ST 3. CONCLUSION: The predominant subtype was ST3 that is the main human subtype. The dominance of ST2 and 5 are important in this study. This superiority has been reported in some of the studies in ST 2 which is different from the studies in other countries, because they have announced priorities of the ST1 and ST6 after ST3. Tehran University of Medical Sciences 2014-03 /pmc/articles/PMC4289879/ /pubmed/25642259 Text en Copyright: © Iranian Journal of Parasitology & Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
BADPARVA, Ebrahim
SADRAEE, Javid
KHEIRANDISH, Farnaz
FROUZANDEH, Mehdi
Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran
title Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran
title_full Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran
title_fullStr Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran
title_full_unstemmed Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran
title_short Genetic Diversity of Human Blastocystis Isolates in Khorramabad, Central Iran
title_sort genetic diversity of human blastocystis isolates in khorramabad, central iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289879/
https://www.ncbi.nlm.nih.gov/pubmed/25642259
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