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In Vitro Study on Cytotoxic Effects of ZnO Nanoparticles on Promastigote and Amastigote Forms of Leishmania major (MRHO/IR/75/ER)

BACKGROUND: Although pentavalent antimony compounds are used as antileishmanial drugs but they are associated with limitations and several adverse complications. Therefore, always effort to find a new and effective treatment is desired. In this study, the effect of ZnO nanoparticles with mean partic...

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Autores principales: DELAVARI, Mahdi, DALIMI, Abdolhossein, GHAFFARIFAR, Fatemeh, SADRAEI, Javid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289881/
https://www.ncbi.nlm.nih.gov/pubmed/25642254
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author DELAVARI, Mahdi
DALIMI, Abdolhossein
GHAFFARIFAR, Fatemeh
SADRAEI, Javid
author_facet DELAVARI, Mahdi
DALIMI, Abdolhossein
GHAFFARIFAR, Fatemeh
SADRAEI, Javid
author_sort DELAVARI, Mahdi
collection PubMed
description BACKGROUND: Although pentavalent antimony compounds are used as antileishmanial drugs but they are associated with limitations and several adverse complications. Therefore, always effort to find a new and effective treatment is desired. In this study, the effect of ZnO nanoparticles with mean particle size of 20 nanometers (nm) on Leishmania major promastigotes and amastigotes was evaluated. METHODS: Viability percentage of promastigotes after adding different concentrations of ZnO nanoparticles (30, 60, 90 and 120 μg/ml) to the parasite culture was evaluated by MTT assay. In the flow cytometry study, Annexin V-FITC Apoptosis detection Kit was used to study the induced apoptosis and necrotic effects. RESULT: IC50 after 24 hours of incubation was 37.8 μg/ml. ZnO nanoparticles exert cytotoxic effects on promastigotes of L. major through the induction of apoptosis. A concentration of 120 μg/ml of ZnO nanoparticles induced 93.76% apoptosis in L. major after 72 hours. CONCLUSION: ZnO NPs can induce apoptosis in L. major by dose and time-depended manner in vitro condition.
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spelling pubmed-42898812015-01-30 In Vitro Study on Cytotoxic Effects of ZnO Nanoparticles on Promastigote and Amastigote Forms of Leishmania major (MRHO/IR/75/ER) DELAVARI, Mahdi DALIMI, Abdolhossein GHAFFARIFAR, Fatemeh SADRAEI, Javid Iran J Parasitol Original Article BACKGROUND: Although pentavalent antimony compounds are used as antileishmanial drugs but they are associated with limitations and several adverse complications. Therefore, always effort to find a new and effective treatment is desired. In this study, the effect of ZnO nanoparticles with mean particle size of 20 nanometers (nm) on Leishmania major promastigotes and amastigotes was evaluated. METHODS: Viability percentage of promastigotes after adding different concentrations of ZnO nanoparticles (30, 60, 90 and 120 μg/ml) to the parasite culture was evaluated by MTT assay. In the flow cytometry study, Annexin V-FITC Apoptosis detection Kit was used to study the induced apoptosis and necrotic effects. RESULT: IC50 after 24 hours of incubation was 37.8 μg/ml. ZnO nanoparticles exert cytotoxic effects on promastigotes of L. major through the induction of apoptosis. A concentration of 120 μg/ml of ZnO nanoparticles induced 93.76% apoptosis in L. major after 72 hours. CONCLUSION: ZnO NPs can induce apoptosis in L. major by dose and time-depended manner in vitro condition. Tehran University of Medical Sciences 2014-03 /pmc/articles/PMC4289881/ /pubmed/25642254 Text en Copyright: © Iranian Journal of Parasitology & Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
DELAVARI, Mahdi
DALIMI, Abdolhossein
GHAFFARIFAR, Fatemeh
SADRAEI, Javid
In Vitro Study on Cytotoxic Effects of ZnO Nanoparticles on Promastigote and Amastigote Forms of Leishmania major (MRHO/IR/75/ER)
title In Vitro Study on Cytotoxic Effects of ZnO Nanoparticles on Promastigote and Amastigote Forms of Leishmania major (MRHO/IR/75/ER)
title_full In Vitro Study on Cytotoxic Effects of ZnO Nanoparticles on Promastigote and Amastigote Forms of Leishmania major (MRHO/IR/75/ER)
title_fullStr In Vitro Study on Cytotoxic Effects of ZnO Nanoparticles on Promastigote and Amastigote Forms of Leishmania major (MRHO/IR/75/ER)
title_full_unstemmed In Vitro Study on Cytotoxic Effects of ZnO Nanoparticles on Promastigote and Amastigote Forms of Leishmania major (MRHO/IR/75/ER)
title_short In Vitro Study on Cytotoxic Effects of ZnO Nanoparticles on Promastigote and Amastigote Forms of Leishmania major (MRHO/IR/75/ER)
title_sort in vitro study on cytotoxic effects of zno nanoparticles on promastigote and amastigote forms of leishmania major (mrho/ir/75/er)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289881/
https://www.ncbi.nlm.nih.gov/pubmed/25642254
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