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A Multi-Locus Study of Cryptosporidium Parasites Isolated From Patients Living In Iran, Malawi, Nigeria, the United Kingdom, and Vietnam
BACKGROUND: Cryptosporidium species are important cause of diarrheal diseases in both developing and developed countries. This study aimed to compare the performance of several molecular methods for identification of Cryptosporidium species, and to detect genetic variation among each of these specie...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289884/ https://www.ncbi.nlm.nih.gov/pubmed/25642263 |
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author | GHAFFARI, Salman KALANTARI, Narges |
author_facet | GHAFFARI, Salman KALANTARI, Narges |
author_sort | GHAFFARI, Salman |
collection | PubMed |
description | BACKGROUND: Cryptosporidium species are important cause of diarrheal diseases in both developing and developed countries. This study aimed to compare the performance of several molecular methods for identification of Cryptosporidium species, and to detect genetic variation among each of these species isolated from Iran, Malawi, Nigeria, Vietnam and the United Kingdom. METHODS: The oocysts DNA samples were derived from 106 Cryptosporidium positive feces. Polymerase chain reaction, PCR- restriction fragment length polymorphism and DNA sequence analysis of the 18S rRNA and the Cryptosporidium oocysts wall protein genes; PCR and DNA sequence analysis of a fragment of 70 kDa heat shock protein and 60 kDa glycoprotein genes were carried out. RESULTS: Based on these analysis, three species of Cryptosporidium including C. hominis, C. parvum and C. meleagridis, and both C. hominis and C. parvum were found in Iranian and the UK samples, respectively. Also, three C. hominis (Ib, Ib3& Id) and three C. parvum (IIa, IIc & IId) subtypes were identified by sequence analysis of the GP60 gene. Of these, C. hominis Ib was predominant and interestingly, one subgenotype (C. hominis Ib A10G2) accounted for the majority of the samples. CONCLUSION: The current study demonstrates the complex subtypes of Cryptosporidium isolates in both developing and developed countries. This is the first report of C. parvum IId subgenotype and three new subtypes of C. parvum IIa in the UK, a new subtype of C. hominis Id from Malawi; and the first multi-locus study of three species of Cryptosporidium in human from Iran. |
format | Online Article Text |
id | pubmed-4289884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-42898842015-01-30 A Multi-Locus Study of Cryptosporidium Parasites Isolated From Patients Living In Iran, Malawi, Nigeria, the United Kingdom, and Vietnam GHAFFARI, Salman KALANTARI, Narges Iran J Parasitol Original Article BACKGROUND: Cryptosporidium species are important cause of diarrheal diseases in both developing and developed countries. This study aimed to compare the performance of several molecular methods for identification of Cryptosporidium species, and to detect genetic variation among each of these species isolated from Iran, Malawi, Nigeria, Vietnam and the United Kingdom. METHODS: The oocysts DNA samples were derived from 106 Cryptosporidium positive feces. Polymerase chain reaction, PCR- restriction fragment length polymorphism and DNA sequence analysis of the 18S rRNA and the Cryptosporidium oocysts wall protein genes; PCR and DNA sequence analysis of a fragment of 70 kDa heat shock protein and 60 kDa glycoprotein genes were carried out. RESULTS: Based on these analysis, three species of Cryptosporidium including C. hominis, C. parvum and C. meleagridis, and both C. hominis and C. parvum were found in Iranian and the UK samples, respectively. Also, three C. hominis (Ib, Ib3& Id) and three C. parvum (IIa, IIc & IId) subtypes were identified by sequence analysis of the GP60 gene. Of these, C. hominis Ib was predominant and interestingly, one subgenotype (C. hominis Ib A10G2) accounted for the majority of the samples. CONCLUSION: The current study demonstrates the complex subtypes of Cryptosporidium isolates in both developing and developed countries. This is the first report of C. parvum IId subgenotype and three new subtypes of C. parvum IIa in the UK, a new subtype of C. hominis Id from Malawi; and the first multi-locus study of three species of Cryptosporidium in human from Iran. Tehran University of Medical Sciences 2014-03 /pmc/articles/PMC4289884/ /pubmed/25642263 Text en Copyright: © Iranian Journal of Parasitology & Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article GHAFFARI, Salman KALANTARI, Narges A Multi-Locus Study of Cryptosporidium Parasites Isolated From Patients Living In Iran, Malawi, Nigeria, the United Kingdom, and Vietnam |
title | A Multi-Locus Study of Cryptosporidium Parasites Isolated From Patients Living In Iran, Malawi, Nigeria, the United Kingdom, and Vietnam |
title_full | A Multi-Locus Study of Cryptosporidium Parasites Isolated From Patients Living In Iran, Malawi, Nigeria, the United Kingdom, and Vietnam |
title_fullStr | A Multi-Locus Study of Cryptosporidium Parasites Isolated From Patients Living In Iran, Malawi, Nigeria, the United Kingdom, and Vietnam |
title_full_unstemmed | A Multi-Locus Study of Cryptosporidium Parasites Isolated From Patients Living In Iran, Malawi, Nigeria, the United Kingdom, and Vietnam |
title_short | A Multi-Locus Study of Cryptosporidium Parasites Isolated From Patients Living In Iran, Malawi, Nigeria, the United Kingdom, and Vietnam |
title_sort | multi-locus study of cryptosporidium parasites isolated from patients living in iran, malawi, nigeria, the united kingdom, and vietnam |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289884/ https://www.ncbi.nlm.nih.gov/pubmed/25642263 |
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