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The effects of disruptions in ribosomal active sites and in intersubunit contacts on ribosomal degradation in Escherichia coli
Although ribosomes are very stable under most conditions, ribosomal degradation does occur in diverse groups of organisms in response to specific stresses or environmental conditions. While non-functional ribosome decay (NRD) in yeast is well characterized, very little is known of the mechanisms tha...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289901/ https://www.ncbi.nlm.nih.gov/pubmed/25578614 http://dx.doi.org/10.1038/srep07712 |
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author | Paier, Anton Leppik, Margus Soosaar, Aksel Tenson, Tanel Maiväli, Ülo |
author_facet | Paier, Anton Leppik, Margus Soosaar, Aksel Tenson, Tanel Maiväli, Ülo |
author_sort | Paier, Anton |
collection | PubMed |
description | Although ribosomes are very stable under most conditions, ribosomal degradation does occur in diverse groups of organisms in response to specific stresses or environmental conditions. While non-functional ribosome decay (NRD) in yeast is well characterized, very little is known of the mechanisms that initiate ribosomal degradation in bacteria. Here we test ribosome degradation in growing Escherichia coli expressing mutant ribosomes. We found that mutations in the 16S rRNA decoding centre (G530U and A1492C) and 23S rRNA active site (A2451G) do not lead to ribosomal degradation. In contrast, 23S rRNA mutation U2585A causes degradation of both the large and small ribosomal subunits in E. coli. We further tested mutations in 23S rRNA, which disrupt ribosomal intersubunit bridges B2a and B3. Deletion of helix 69 of 23S rRNA and the point mutation A1912G in the same helix did not destabilize ribosomes, while expression of mutations A1919G in H69 and A1960G in H71 led to degradation of both mutant and wild-type ribosomes. Our results suggest an actively induced mechanism requiring de novo protein synthesis for ribosomal degradation in E. coli, which degrades both structurally inactive and active ribosomes. |
format | Online Article Text |
id | pubmed-4289901 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-42899012015-01-16 The effects of disruptions in ribosomal active sites and in intersubunit contacts on ribosomal degradation in Escherichia coli Paier, Anton Leppik, Margus Soosaar, Aksel Tenson, Tanel Maiväli, Ülo Sci Rep Article Although ribosomes are very stable under most conditions, ribosomal degradation does occur in diverse groups of organisms in response to specific stresses or environmental conditions. While non-functional ribosome decay (NRD) in yeast is well characterized, very little is known of the mechanisms that initiate ribosomal degradation in bacteria. Here we test ribosome degradation in growing Escherichia coli expressing mutant ribosomes. We found that mutations in the 16S rRNA decoding centre (G530U and A1492C) and 23S rRNA active site (A2451G) do not lead to ribosomal degradation. In contrast, 23S rRNA mutation U2585A causes degradation of both the large and small ribosomal subunits in E. coli. We further tested mutations in 23S rRNA, which disrupt ribosomal intersubunit bridges B2a and B3. Deletion of helix 69 of 23S rRNA and the point mutation A1912G in the same helix did not destabilize ribosomes, while expression of mutations A1919G in H69 and A1960G in H71 led to degradation of both mutant and wild-type ribosomes. Our results suggest an actively induced mechanism requiring de novo protein synthesis for ribosomal degradation in E. coli, which degrades both structurally inactive and active ribosomes. Nature Publishing Group 2015-01-12 /pmc/articles/PMC4289901/ /pubmed/25578614 http://dx.doi.org/10.1038/srep07712 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Paier, Anton Leppik, Margus Soosaar, Aksel Tenson, Tanel Maiväli, Ülo The effects of disruptions in ribosomal active sites and in intersubunit contacts on ribosomal degradation in Escherichia coli |
title | The effects of disruptions in ribosomal active sites and in intersubunit contacts on ribosomal degradation in Escherichia coli |
title_full | The effects of disruptions in ribosomal active sites and in intersubunit contacts on ribosomal degradation in Escherichia coli |
title_fullStr | The effects of disruptions in ribosomal active sites and in intersubunit contacts on ribosomal degradation in Escherichia coli |
title_full_unstemmed | The effects of disruptions in ribosomal active sites and in intersubunit contacts on ribosomal degradation in Escherichia coli |
title_short | The effects of disruptions in ribosomal active sites and in intersubunit contacts on ribosomal degradation in Escherichia coli |
title_sort | effects of disruptions in ribosomal active sites and in intersubunit contacts on ribosomal degradation in escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289901/ https://www.ncbi.nlm.nih.gov/pubmed/25578614 http://dx.doi.org/10.1038/srep07712 |
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