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Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides

Polyvinyl chloride (PVC) can be used to develop reaction beaker which acts as electrochemical cell for the measurement of OP pesticides. Being chemically inert, corrosion resistant, and easy in molding to various shapes and size, PVC can be used for the immobilization of enzyme. Organophosphorus hyd...

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Autores principales: Gothwal, Ashish, Beniwal, Puneet, Dhull, Vikas, Hooda, Vikas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4290034/
https://www.ncbi.nlm.nih.gov/pubmed/25667593
http://dx.doi.org/10.1155/2014/303641
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author Gothwal, Ashish
Beniwal, Puneet
Dhull, Vikas
Hooda, Vikas
author_facet Gothwal, Ashish
Beniwal, Puneet
Dhull, Vikas
Hooda, Vikas
author_sort Gothwal, Ashish
collection PubMed
description Polyvinyl chloride (PVC) can be used to develop reaction beaker which acts as electrochemical cell for the measurement of OP pesticides. Being chemically inert, corrosion resistant, and easy in molding to various shapes and size, PVC can be used for the immobilization of enzyme. Organophosphorus hydrolase was immobilized covalently onto the chemically activated inner surface of PVC beaker by using glutaraldehyde as a coupling agent. The carbon nanotubes paste working electrode was constructed for amperometric measurement at a potential of +0.8 V. The biosensor showed optimum response at pH 8.0 with incubation temperature of 40°C. K (m) and I (max) for substrate (methyl parathion) were 322.58 µM and 1.1 µA, respectively. Evaluation study showed a correlation of 0.985, which was in agreement with the standard method. The OPH biosensor lost 50% of its initial activity after its regular use for 25 times over a period of 50 days when stored in 0.1 M sodium phosphate buffer, pH 8.0 at 4°C. No interference was observed by interfering species.
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spelling pubmed-42900342015-02-09 Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides Gothwal, Ashish Beniwal, Puneet Dhull, Vikas Hooda, Vikas Int J Anal Chem Research Article Polyvinyl chloride (PVC) can be used to develop reaction beaker which acts as electrochemical cell for the measurement of OP pesticides. Being chemically inert, corrosion resistant, and easy in molding to various shapes and size, PVC can be used for the immobilization of enzyme. Organophosphorus hydrolase was immobilized covalently onto the chemically activated inner surface of PVC beaker by using glutaraldehyde as a coupling agent. The carbon nanotubes paste working electrode was constructed for amperometric measurement at a potential of +0.8 V. The biosensor showed optimum response at pH 8.0 with incubation temperature of 40°C. K (m) and I (max) for substrate (methyl parathion) were 322.58 µM and 1.1 µA, respectively. Evaluation study showed a correlation of 0.985, which was in agreement with the standard method. The OPH biosensor lost 50% of its initial activity after its regular use for 25 times over a period of 50 days when stored in 0.1 M sodium phosphate buffer, pH 8.0 at 4°C. No interference was observed by interfering species. Hindawi Publishing Corporation 2014 2014-12-24 /pmc/articles/PMC4290034/ /pubmed/25667593 http://dx.doi.org/10.1155/2014/303641 Text en Copyright © 2014 Ashish Gothwal et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gothwal, Ashish
Beniwal, Puneet
Dhull, Vikas
Hooda, Vikas
Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides
title Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides
title_full Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides
title_fullStr Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides
title_full_unstemmed Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides
title_short Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides
title_sort preparation of electrochemical biosensor for detection of organophosphorus pesticides
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4290034/
https://www.ncbi.nlm.nih.gov/pubmed/25667593
http://dx.doi.org/10.1155/2014/303641
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