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MD-2 is required for disulfide HMGB1–dependent TLR4 signaling

Innate immune receptors for pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) orchestrate inflammatory responses to infection and injury. Secreted by activated immune cells or passively released by damaged cells, HMGB1 is subjected to redox modification that distinctly influences...

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Autores principales: Yang, Huan, Wang, Haichao, Ju, Zhongliang, Ragab, Ahmed A., Lundbäck, Peter, Long, Wei, Valdes-Ferrer, Sergio I., He, Mingzhu, Pribis, John P., Li, Jianhua, Lu, Ben, Gero, Domokos, Szabo, Csaba, Antoine, Daniel J., Harris, Helena E., Golenbock, Doug T., Meng, Jianmin, Roth, Jesse, Chavan, Sangeeta S., Andersson, Ulf, Billiar, Timothy R., Tracey, Kevin J., Al-Abed, Yousef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4291531/
https://www.ncbi.nlm.nih.gov/pubmed/25559892
http://dx.doi.org/10.1084/jem.20141318
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author Yang, Huan
Wang, Haichao
Ju, Zhongliang
Ragab, Ahmed A.
Lundbäck, Peter
Long, Wei
Valdes-Ferrer, Sergio I.
He, Mingzhu
Pribis, John P.
Li, Jianhua
Lu, Ben
Gero, Domokos
Szabo, Csaba
Antoine, Daniel J.
Harris, Helena E.
Golenbock, Doug T.
Meng, Jianmin
Roth, Jesse
Chavan, Sangeeta S.
Andersson, Ulf
Billiar, Timothy R.
Tracey, Kevin J.
Al-Abed, Yousef
author_facet Yang, Huan
Wang, Haichao
Ju, Zhongliang
Ragab, Ahmed A.
Lundbäck, Peter
Long, Wei
Valdes-Ferrer, Sergio I.
He, Mingzhu
Pribis, John P.
Li, Jianhua
Lu, Ben
Gero, Domokos
Szabo, Csaba
Antoine, Daniel J.
Harris, Helena E.
Golenbock, Doug T.
Meng, Jianmin
Roth, Jesse
Chavan, Sangeeta S.
Andersson, Ulf
Billiar, Timothy R.
Tracey, Kevin J.
Al-Abed, Yousef
author_sort Yang, Huan
collection PubMed
description Innate immune receptors for pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) orchestrate inflammatory responses to infection and injury. Secreted by activated immune cells or passively released by damaged cells, HMGB1 is subjected to redox modification that distinctly influences its extracellular functions. Previously, it was unknown how the TLR4 signalosome distinguished between HMGB1 isoforms. Here we demonstrate that the extracellular TLR4 adaptor, myeloid differentiation factor 2 (MD-2), binds specifically to the cytokine-inducing disulfide isoform of HMGB1, to the exclusion of other isoforms. Using MD-2–deficient mice, as well as MD-2 silencing in macrophages, we show a requirement for HMGB1-dependent TLR4 signaling. By screening HMGB1 peptide libraries, we identified a tetramer (FSSE, designated P5779) as a specific MD-2 antagonist preventing MD-2–HMGB1 interaction and TLR4 signaling. P5779 does not interfere with lipopolysaccharide-induced cytokine/chemokine production, thus preserving PAMP-mediated TLR4–MD-2 responses. Furthermore, P5779 can protect mice against hepatic ischemia/reperfusion injury, chemical toxicity, and sepsis. These findings reveal a novel mechanism by which innate systems selectively recognize specific HMGB1 isoforms. The results may direct toward strategies aimed at attenuating DAMP-mediated inflammation while preserving antimicrobial immune responsiveness.
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spelling pubmed-42915312015-07-12 MD-2 is required for disulfide HMGB1–dependent TLR4 signaling Yang, Huan Wang, Haichao Ju, Zhongliang Ragab, Ahmed A. Lundbäck, Peter Long, Wei Valdes-Ferrer, Sergio I. He, Mingzhu Pribis, John P. Li, Jianhua Lu, Ben Gero, Domokos Szabo, Csaba Antoine, Daniel J. Harris, Helena E. Golenbock, Doug T. Meng, Jianmin Roth, Jesse Chavan, Sangeeta S. Andersson, Ulf Billiar, Timothy R. Tracey, Kevin J. Al-Abed, Yousef J Exp Med Brief Definitive Report Innate immune receptors for pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) orchestrate inflammatory responses to infection and injury. Secreted by activated immune cells or passively released by damaged cells, HMGB1 is subjected to redox modification that distinctly influences its extracellular functions. Previously, it was unknown how the TLR4 signalosome distinguished between HMGB1 isoforms. Here we demonstrate that the extracellular TLR4 adaptor, myeloid differentiation factor 2 (MD-2), binds specifically to the cytokine-inducing disulfide isoform of HMGB1, to the exclusion of other isoforms. Using MD-2–deficient mice, as well as MD-2 silencing in macrophages, we show a requirement for HMGB1-dependent TLR4 signaling. By screening HMGB1 peptide libraries, we identified a tetramer (FSSE, designated P5779) as a specific MD-2 antagonist preventing MD-2–HMGB1 interaction and TLR4 signaling. P5779 does not interfere with lipopolysaccharide-induced cytokine/chemokine production, thus preserving PAMP-mediated TLR4–MD-2 responses. Furthermore, P5779 can protect mice against hepatic ischemia/reperfusion injury, chemical toxicity, and sepsis. These findings reveal a novel mechanism by which innate systems selectively recognize specific HMGB1 isoforms. The results may direct toward strategies aimed at attenuating DAMP-mediated inflammation while preserving antimicrobial immune responsiveness. The Rockefeller University Press 2015-01-12 /pmc/articles/PMC4291531/ /pubmed/25559892 http://dx.doi.org/10.1084/jem.20141318 Text en © 2015 Yang et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Brief Definitive Report
Yang, Huan
Wang, Haichao
Ju, Zhongliang
Ragab, Ahmed A.
Lundbäck, Peter
Long, Wei
Valdes-Ferrer, Sergio I.
He, Mingzhu
Pribis, John P.
Li, Jianhua
Lu, Ben
Gero, Domokos
Szabo, Csaba
Antoine, Daniel J.
Harris, Helena E.
Golenbock, Doug T.
Meng, Jianmin
Roth, Jesse
Chavan, Sangeeta S.
Andersson, Ulf
Billiar, Timothy R.
Tracey, Kevin J.
Al-Abed, Yousef
MD-2 is required for disulfide HMGB1–dependent TLR4 signaling
title MD-2 is required for disulfide HMGB1–dependent TLR4 signaling
title_full MD-2 is required for disulfide HMGB1–dependent TLR4 signaling
title_fullStr MD-2 is required for disulfide HMGB1–dependent TLR4 signaling
title_full_unstemmed MD-2 is required for disulfide HMGB1–dependent TLR4 signaling
title_short MD-2 is required for disulfide HMGB1–dependent TLR4 signaling
title_sort md-2 is required for disulfide hmgb1–dependent tlr4 signaling
topic Brief Definitive Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4291531/
https://www.ncbi.nlm.nih.gov/pubmed/25559892
http://dx.doi.org/10.1084/jem.20141318
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