Cargando…

Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography

Chloroplast function is orchestrated by the organelle's intricate architecture. By combining cryo-focused ion beam milling of vitreous Chlamydomonas cells with cryo-electron tomography, we acquired three-dimensional structures of the chloroplast in its native state within the cell. Chloroplast...

Descripción completa

Detalles Bibliográficos
Autores principales:	Engel, Benjamin D, Schaffer, Miroslava, Kuhn Cuellar, Luis, Villa, Elizabeth, Plitzko, Jürgen M, Baumeister, Wolfgang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	eLife Sciences Publications, Ltd 2015
Materias:	Biophysics and Structural Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4292175/ https://www.ncbi.nlm.nih.gov/pubmed/25584625 http://dx.doi.org/10.7554/eLife.04889

Ejemplares similares

Correction: Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography
por: Engel, Benjamin D, et al.
Publicado: (2015)

Charting the native architecture of Chlamydomonas thylakoid membranes with single-molecule precision
por: Wietrzynski, Wojciech, et al.
Publicado: (2020)

The structure of the COPI coat determined within the cell
por: Bykov, Yury S, et al.
Publicado: (2017)

Cryo-EM single particle analysis with the Volta phase plate
por: Danev, Radostin, et al.
Publicado: (2016)

Using the Volta phase plate with defocus for cryo-EM single particle analysis
por: Danev, Radostin, et al.
Publicado: (2017)

In situ Microfluidic Cryofixation for Cryo Focused Ion Beam Milling and Cryo Electron Tomography
por: Fuest, Marie, et al.
Publicado: (2019)

A modular platform for automated cryo-FIB workflows
por: Klumpe, Sven, et al.
Publicado: (2021)

In situ architecture of the algal nuclear pore complex
por: Mosalaganti, Shyamal, et al.
Publicado: (2018)

Cryo-EM structures of an LRRC8 chimera with native functional properties reveal heptameric assembly
por: Takahashi, Hirohide, et al.
Publicado: (2023)

Dynamic curvature regulation accounts for the symmetric and asymmetric beats of Chlamydomonas flagella
por: Sartori, Pablo, et al.
Publicado: (2016)

In situ cryo-electron tomography reveals gradient organization of ribosome biogenesis in intact nucleoli
por: Erdmann, Philipp S., et al.
Publicado: (2021)

Routine single particle CryoEM sample and grid characterization by tomography
por: Noble, Alex J, et al.
Publicado: (2018)

Cryo-EM enters a new era
por: Kühlbrandt, Werner
Publicado: (2014)

Fully automated, sequential focused ion beam milling for cryo-electron tomography
por: Zachs, Tobias, et al.
Publicado: (2020)

Direct detection pays off for electron cryo-microscopy
por: Grigorieff, Nikolaus
Publicado: (2013)

Cryo-EM structure in situ reveals a molecular switch that safeguards virus against genome loss
por: Bayfield, Oliver W, et al.
Publicado: (2020)

A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0
por: Zivanov, Jasenko, et al.
Publicado: (2022)

Beam-induced motion correction for sub-megadalton cryo-EM particles
por: Scheres, Sjors HW
Publicado: (2014)

Single-protein detection in crowded molecular environments in cryo-EM images
por: Rickgauer, J Peter, et al.
Publicado: (2017)

The internal plumbing of algal chloroplasts
por: Meyer, Moritz, et al.
Publicado: (2015)

Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2
por: Kimanius, Dari, et al.
Publicado: (2016)

Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles
por: Bai, Xiao-chen, et al.
Publicado: (2013)

Cryo-EM structures of the autoinhibited E. coli ATP synthase in three rotational states
por: Sobti, Meghna, et al.
Publicado: (2016)

Automated structure refinement of macromolecular assemblies from cryo-EM maps using Rosetta
por: Wang, Ray Yu-Ruei, et al.
Publicado: (2016)

Cryo-EM structure of the ATP-sensitive potassium channel illuminates mechanisms of assembly and gating
por: Martin, Gregory M, et al.
Publicado: (2017)

CryoEM structures of membrane pore and prepore complex reveal cytolytic mechanism of Pneumolysin
por: van Pee, Katharina, et al.
Publicado: (2017)

Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine
por: Wong, Wilson, et al.
Publicado: (2014)

Low cost, high performance processing of single particle cryo-electron microscopy data in the cloud
por: Cianfrocco, Michael A, et al.
Publicado: (2015)

Molecular dynamics-based refinement and validation for sub-5 Å cryo-electron microscopy maps
por: Singharoy, Abhishek, et al.
Publicado: (2016)

2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy
por: Campbell, Melody G, et al.
Publicado: (2015)

Molecular architecture of the yeast Mediator complex
por: Robinson, Philip J, et al.
Publicado: (2015)

Visualizing the functional architecture of the endocytic machinery
por: Picco, Andrea, et al.
Publicado: (2015)

De novo modeling of the F(420)-reducing [NiFe]-hydrogenase from a methanogenic archaeon by cryo-electron microscopy
por: Mills, Deryck J, et al.
Publicado: (2013)

A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5 Å resolution
por: Zhang, Xing, et al.
Publicado: (2013)

Atomic model of the F(420)-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector
por: Allegretti, Matteo, et al.
Publicado: (2014)

Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6
por: Grant, Timothy, et al.
Publicado: (2015)

Correction: A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5 Å resolution
por: Zhang, Xing, et al.
Publicado: (2015)

cryo-EM structures of the E. coli replicative DNA polymerase reveal its dynamic interactions with the DNA sliding clamp, exonuclease and τ
por: Fernandez-Leiro, Rafael, et al.
Publicado: (2015)

In situ structural analysis of the Yersinia enterocolitica injectisome
por: Kudryashev, Mikhail, et al.
Publicado: (2013)

The near-atomic cryoEM structure of a flexible filamentous plant virus shows homology of its coat protein with nucleoproteins of animal viruses
por: Agirrezabala, Xabier, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_nl1i3nkt34st9jog7r1nd1d7ig