A peptide from Porphyra yezoensis stimulates the proliferation of IEC-6 cells by activating the insulin-like growth factor I receptor signaling pathway

Porphyra yezoensis (P. yezoensis) is the most noteworthy red alga and is mainly consumed in China, Japan and Korea. In the present study, the effects of a P. yezoensis peptide (PY-PE) on cell proliferation and the associated signaling pathways were examined in IEC-6 rat intestinal epithelial cells. First, the MTS assay showed that PY-PE induced cell proliferation in a dose-dependent manner. Subsequently, the mechanism behind the proliferative activity induced by PY-PE was determined. The insulin-like growth factor-I receptor (IGF-IR) signaling pathway was the main focus as it plays an important role in the regulation of cell growth and proliferation. PY-PE increased the protein and mRNA expression of IGF-IR, insulin receptor substrate-1, Shc and PY-99. In addition, PY-PE stimulated extracellular signal-regulated kinase phosphorylation and phosphatidylinositol 3-kinase/Akt activation but inhibited p38 and c-Jun N-terminal kinase phosphorylation. Furthermore, PY-PE treatment increased protein and mRNA expression levels of activator protein-1, which regulates cell proliferation and survival, in the nuclear fraction. These results have significant implications for understanding the role of cell proliferation signaling pathways in intestinal epithelial cells.