Lmo0171, a Novel Internalin-Like Protein, Determines Cell Morphology of Listeria monocytogenes and Its Ability to Invade Human Cell Lines

Internalins comprise a class of Listeria monocytogenes proteins responsible for activation of signalling pathways leading to phagocytic uptake of the bacterium by the host cell. In this paper, a possible role of Lmo0171—a new member of the internalin family was investigated. Disruption of the lmo017...

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Autores principales: Stachowiak, Radosław, Jagielski, Tomasz, Roeske, Katarzyna, Osińska, Olga, Gunerka, Paweł, Wiśniewski, Jarosław, Bielecki, Jacek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4293459/
https://www.ncbi.nlm.nih.gov/pubmed/25323012
http://dx.doi.org/10.1007/s00284-014-0715-4
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author Stachowiak, Radosław
Jagielski, Tomasz
Roeske, Katarzyna
Osińska, Olga
Gunerka, Paweł
Wiśniewski, Jarosław
Bielecki, Jacek
author_facet Stachowiak, Radosław
Jagielski, Tomasz
Roeske, Katarzyna
Osińska, Olga
Gunerka, Paweł
Wiśniewski, Jarosław
Bielecki, Jacek
author_sort Stachowiak, Radosław
collection PubMed
description Internalins comprise a class of Listeria monocytogenes proteins responsible for activation of signalling pathways leading to phagocytic uptake of the bacterium by the host cell. In this paper, a possible role of Lmo0171—a new member of the internalin family was investigated. Disruption of the lmo0171 gene resulted in important cell morphology alterations along with a decrease in the ability to invade three eukaryotic cell lines, that is Int407, Hep-2 and HeLa and diminished adhesion efficiency to int407, thereby suggesting bifunctionality of the newly characterised Lmo0171 internalin.
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spelling pubmed-42934592015-01-21 Lmo0171, a Novel Internalin-Like Protein, Determines Cell Morphology of Listeria monocytogenes and Its Ability to Invade Human Cell Lines Stachowiak, Radosław Jagielski, Tomasz Roeske, Katarzyna Osińska, Olga Gunerka, Paweł Wiśniewski, Jarosław Bielecki, Jacek Curr Microbiol Article Internalins comprise a class of Listeria monocytogenes proteins responsible for activation of signalling pathways leading to phagocytic uptake of the bacterium by the host cell. In this paper, a possible role of Lmo0171—a new member of the internalin family was investigated. Disruption of the lmo0171 gene resulted in important cell morphology alterations along with a decrease in the ability to invade three eukaryotic cell lines, that is Int407, Hep-2 and HeLa and diminished adhesion efficiency to int407, thereby suggesting bifunctionality of the newly characterised Lmo0171 internalin. Springer US 2014-10-17 2015 /pmc/articles/PMC4293459/ /pubmed/25323012 http://dx.doi.org/10.1007/s00284-014-0715-4 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Stachowiak, Radosław
Jagielski, Tomasz
Roeske, Katarzyna
Osińska, Olga
Gunerka, Paweł
Wiśniewski, Jarosław
Bielecki, Jacek
Lmo0171, a Novel Internalin-Like Protein, Determines Cell Morphology of Listeria monocytogenes and Its Ability to Invade Human Cell Lines
title Lmo0171, a Novel Internalin-Like Protein, Determines Cell Morphology of Listeria monocytogenes and Its Ability to Invade Human Cell Lines
title_full Lmo0171, a Novel Internalin-Like Protein, Determines Cell Morphology of Listeria monocytogenes and Its Ability to Invade Human Cell Lines
title_fullStr Lmo0171, a Novel Internalin-Like Protein, Determines Cell Morphology of Listeria monocytogenes and Its Ability to Invade Human Cell Lines
title_full_unstemmed Lmo0171, a Novel Internalin-Like Protein, Determines Cell Morphology of Listeria monocytogenes and Its Ability to Invade Human Cell Lines
title_short Lmo0171, a Novel Internalin-Like Protein, Determines Cell Morphology of Listeria monocytogenes and Its Ability to Invade Human Cell Lines
title_sort lmo0171, a novel internalin-like protein, determines cell morphology of listeria monocytogenes and its ability to invade human cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4293459/
https://www.ncbi.nlm.nih.gov/pubmed/25323012
http://dx.doi.org/10.1007/s00284-014-0715-4
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