Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus

The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux ‘threshold’, with the frequency of the ‘threshold crossings’ correlating...

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Detalles Bibliográficos
Autores principales: Christo, Susan N., Diener, Kerrilyn R., Nordon, Robert E., Brown, Michael P., Griesser, Hans J., Vasilev, Krasimir, Christo, Farid C., Hayball, John D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4293621/
https://www.ncbi.nlm.nih.gov/pubmed/25585590
http://dx.doi.org/10.1038/srep07760
Descripción
Sumario:The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux ‘threshold’, with the frequency of the ‘threshold crossings’ correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.

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