Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus

The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux ‘threshold’, with the frequency of the ‘threshold crossings’ correlating...

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Autores principales: Christo, Susan N., Diener, Kerrilyn R., Nordon, Robert E., Brown, Michael P., Griesser, Hans J., Vasilev, Krasimir, Christo, Farid C., Hayball, John D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4293621/
https://www.ncbi.nlm.nih.gov/pubmed/25585590
http://dx.doi.org/10.1038/srep07760
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author Christo, Susan N.
Diener, Kerrilyn R.
Nordon, Robert E.
Brown, Michael P.
Griesser, Hans J.
Vasilev, Krasimir
Christo, Farid C.
Hayball, John D.
author_facet Christo, Susan N.
Diener, Kerrilyn R.
Nordon, Robert E.
Brown, Michael P.
Griesser, Hans J.
Vasilev, Krasimir
Christo, Farid C.
Hayball, John D.
author_sort Christo, Susan N.
collection PubMed
description The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux ‘threshold’, with the frequency of the ‘threshold crossings’ correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.
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spelling pubmed-42936212015-01-27 Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus Christo, Susan N. Diener, Kerrilyn R. Nordon, Robert E. Brown, Michael P. Griesser, Hans J. Vasilev, Krasimir Christo, Farid C. Hayball, John D. Sci Rep Article The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux ‘threshold’, with the frequency of the ‘threshold crossings’ correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level. Nature Publishing Group 2015-01-14 /pmc/articles/PMC4293621/ /pubmed/25585590 http://dx.doi.org/10.1038/srep07760 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Christo, Susan N.
Diener, Kerrilyn R.
Nordon, Robert E.
Brown, Michael P.
Griesser, Hans J.
Vasilev, Krasimir
Christo, Farid C.
Hayball, John D.
Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus
title Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus
title_full Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus
title_fullStr Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus
title_full_unstemmed Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus
title_short Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus
title_sort scrutinizing calcium flux oscillations in t lymphocytes to deduce the strength of stimulus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4293621/
https://www.ncbi.nlm.nih.gov/pubmed/25585590
http://dx.doi.org/10.1038/srep07760
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