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Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis

Human β-defensin 2 (HBD-2) is an antimicrobial peptide produced by mucosal surfaces in response to microbial exposure or inflammatory cytokines. Although HBD-2 is expressed in the esophagus in response to stress and infectious agents, little is known regarding its expression and functional role in e...

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Autores principales: Shi, Ni, Jin, Feng, Zhang, Xiaoli, Clinton, Steven K., Pan, Zui, Chen, Tong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294379/
https://www.ncbi.nlm.nih.gov/pubmed/25226614
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author Shi, Ni
Jin, Feng
Zhang, Xiaoli
Clinton, Steven K.
Pan, Zui
Chen, Tong
author_facet Shi, Ni
Jin, Feng
Zhang, Xiaoli
Clinton, Steven K.
Pan, Zui
Chen, Tong
author_sort Shi, Ni
collection PubMed
description Human β-defensin 2 (HBD-2) is an antimicrobial peptide produced by mucosal surfaces in response to microbial exposure or inflammatory cytokines. Although HBD-2 is expressed in the esophagus in response to stress and infectious agents, little is known regarding its expression and functional role in esophageal carcinogenesis. In the current investigation, normal esophagus and N-nitrosomethylbenzylamine (NMBA)-induced precancerous and papillomatous lesions of the rat esophagus were characterized for HBD-2 encoding gene Defb4 and protein. HBD-2 was found to be overexpressed in esophagi of rats treated with NMBA compared to animals in control group. Results of Real-time PCR, Western blot and immunohistochemistry demonstrated a positive correlation between the overexpression of HBD-2 and the progression of rat squamous cell carcinogenesis (SCC) in the esophagus. We also observed that HBD-2 is overexpressed in tumor tissues removed from patients with esophageal SCC. Moreover, Defb4 silencing in vitro suppresses the tumor cell proliferation, mobility and invasion in esophageal SCC cell line KYSE-150. The results from this study provide experimental evidence that HBD-2 may play an oncogenic role in the initiation and progression of esophageal SCC and thus serves as a target for chemopreventive and therapeutic interventions.
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spelling pubmed-42943792015-01-21 Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis Shi, Ni Jin, Feng Zhang, Xiaoli Clinton, Steven K. Pan, Zui Chen, Tong Oncotarget Research Paper Human β-defensin 2 (HBD-2) is an antimicrobial peptide produced by mucosal surfaces in response to microbial exposure or inflammatory cytokines. Although HBD-2 is expressed in the esophagus in response to stress and infectious agents, little is known regarding its expression and functional role in esophageal carcinogenesis. In the current investigation, normal esophagus and N-nitrosomethylbenzylamine (NMBA)-induced precancerous and papillomatous lesions of the rat esophagus were characterized for HBD-2 encoding gene Defb4 and protein. HBD-2 was found to be overexpressed in esophagi of rats treated with NMBA compared to animals in control group. Results of Real-time PCR, Western blot and immunohistochemistry demonstrated a positive correlation between the overexpression of HBD-2 and the progression of rat squamous cell carcinogenesis (SCC) in the esophagus. We also observed that HBD-2 is overexpressed in tumor tissues removed from patients with esophageal SCC. Moreover, Defb4 silencing in vitro suppresses the tumor cell proliferation, mobility and invasion in esophageal SCC cell line KYSE-150. The results from this study provide experimental evidence that HBD-2 may play an oncogenic role in the initiation and progression of esophageal SCC and thus serves as a target for chemopreventive and therapeutic interventions. Impact Journals LLC 2014-09-05 /pmc/articles/PMC4294379/ /pubmed/25226614 Text en Copyright: © 2014 Shi et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
spellingShingle Research Paper
Shi, Ni
Jin, Feng
Zhang, Xiaoli
Clinton, Steven K.
Pan, Zui
Chen, Tong
Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis
title Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis
title_full Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis
title_fullStr Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis
title_full_unstemmed Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis
title_short Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis
title_sort overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294379/
https://www.ncbi.nlm.nih.gov/pubmed/25226614
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