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Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis
Human β-defensin 2 (HBD-2) is an antimicrobial peptide produced by mucosal surfaces in response to microbial exposure or inflammatory cytokines. Although HBD-2 is expressed in the esophagus in response to stress and infectious agents, little is known regarding its expression and functional role in e...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294379/ https://www.ncbi.nlm.nih.gov/pubmed/25226614 |
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author | Shi, Ni Jin, Feng Zhang, Xiaoli Clinton, Steven K. Pan, Zui Chen, Tong |
author_facet | Shi, Ni Jin, Feng Zhang, Xiaoli Clinton, Steven K. Pan, Zui Chen, Tong |
author_sort | Shi, Ni |
collection | PubMed |
description | Human β-defensin 2 (HBD-2) is an antimicrobial peptide produced by mucosal surfaces in response to microbial exposure or inflammatory cytokines. Although HBD-2 is expressed in the esophagus in response to stress and infectious agents, little is known regarding its expression and functional role in esophageal carcinogenesis. In the current investigation, normal esophagus and N-nitrosomethylbenzylamine (NMBA)-induced precancerous and papillomatous lesions of the rat esophagus were characterized for HBD-2 encoding gene Defb4 and protein. HBD-2 was found to be overexpressed in esophagi of rats treated with NMBA compared to animals in control group. Results of Real-time PCR, Western blot and immunohistochemistry demonstrated a positive correlation between the overexpression of HBD-2 and the progression of rat squamous cell carcinogenesis (SCC) in the esophagus. We also observed that HBD-2 is overexpressed in tumor tissues removed from patients with esophageal SCC. Moreover, Defb4 silencing in vitro suppresses the tumor cell proliferation, mobility and invasion in esophageal SCC cell line KYSE-150. The results from this study provide experimental evidence that HBD-2 may play an oncogenic role in the initiation and progression of esophageal SCC and thus serves as a target for chemopreventive and therapeutic interventions. |
format | Online Article Text |
id | pubmed-4294379 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-42943792015-01-21 Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis Shi, Ni Jin, Feng Zhang, Xiaoli Clinton, Steven K. Pan, Zui Chen, Tong Oncotarget Research Paper Human β-defensin 2 (HBD-2) is an antimicrobial peptide produced by mucosal surfaces in response to microbial exposure or inflammatory cytokines. Although HBD-2 is expressed in the esophagus in response to stress and infectious agents, little is known regarding its expression and functional role in esophageal carcinogenesis. In the current investigation, normal esophagus and N-nitrosomethylbenzylamine (NMBA)-induced precancerous and papillomatous lesions of the rat esophagus were characterized for HBD-2 encoding gene Defb4 and protein. HBD-2 was found to be overexpressed in esophagi of rats treated with NMBA compared to animals in control group. Results of Real-time PCR, Western blot and immunohistochemistry demonstrated a positive correlation between the overexpression of HBD-2 and the progression of rat squamous cell carcinogenesis (SCC) in the esophagus. We also observed that HBD-2 is overexpressed in tumor tissues removed from patients with esophageal SCC. Moreover, Defb4 silencing in vitro suppresses the tumor cell proliferation, mobility and invasion in esophageal SCC cell line KYSE-150. The results from this study provide experimental evidence that HBD-2 may play an oncogenic role in the initiation and progression of esophageal SCC and thus serves as a target for chemopreventive and therapeutic interventions. Impact Journals LLC 2014-09-05 /pmc/articles/PMC4294379/ /pubmed/25226614 Text en Copyright: © 2014 Shi et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
spellingShingle | Research Paper Shi, Ni Jin, Feng Zhang, Xiaoli Clinton, Steven K. Pan, Zui Chen, Tong Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis |
title | Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis |
title_full | Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis |
title_fullStr | Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis |
title_full_unstemmed | Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis |
title_short | Overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis |
title_sort | overexpression of human β-defensin 2 promotes growth and invasion during esophageal carcinogenesis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294379/ https://www.ncbi.nlm.nih.gov/pubmed/25226614 |
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