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Efficient in vitro refolding and functional characterization of recombinant human liver carboxylesterase (CES1) expressed in E. coli

Human liver carboxylesterase 1 (CES1) plays a critical role in the hydrolysis of various ester- and amide-containing molecules, including active metabolites, drugs and prodrugs. However, it has been problematic to express recombinant CES1 in bacterial expression systems due to low solubility, with t...

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Detalles Bibliográficos
Autores principales:	Boonyuen, Usa, Promnares, Kamoltip, Junkree, Suwapat, Day, Nichloas P.J., Imwong, Mallika
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Academic Press 2015
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294421/ https://www.ncbi.nlm.nih.gov/pubmed/25462813 http://dx.doi.org/10.1016/j.pep.2014.11.006

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