Optimized cell transplantation using adult rag2 mutant zebrafish

Cell transplantation into adult zebrafish has lagged behind mouse due to the lack of immune compromised models. Here, we have created homozygous rag2(E450fs) mutant zebrafish that have reduced numbers of functional T and B cells but are viable and fecund. Mutant fish engraft zebrafish muscle, blood...

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Detalles Bibliográficos
Autores principales: Tang, Qin, Abdelfattah, Nouran S., Blackburn, Jessica S., Moore, John C., Martinez, Sarah A., Moore, Finola E., Lobbardi, Riadh, Tenente, Inês M., Ignatius, Myron S., Berman, Jason N., Liwski, Robert S., Houvras, Yariv, Langenau, David M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294527/
https://www.ncbi.nlm.nih.gov/pubmed/25042784
http://dx.doi.org/10.1038/nmeth.3031
Descripción
Sumario:Cell transplantation into adult zebrafish has lagged behind mouse due to the lack of immune compromised models. Here, we have created homozygous rag2(E450fs) mutant zebrafish that have reduced numbers of functional T and B cells but are viable and fecund. Mutant fish engraft zebrafish muscle, blood stem cells, and cancers. rag2(E450fs) mutant zebrafish are the first immune compromised zebrafish model that permits robust, long-term engraftment of multiple tissues and cancer.

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