Optimized cell transplantation using adult rag2 mutant zebrafish

Cell transplantation into adult zebrafish has lagged behind mouse due to the lack of immune compromised models. Here, we have created homozygous rag2(E450fs) mutant zebrafish that have reduced numbers of functional T and B cells but are viable and fecund. Mutant fish engraft zebrafish muscle, blood...

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Autores principales: Tang, Qin, Abdelfattah, Nouran S., Blackburn, Jessica S., Moore, John C., Martinez, Sarah A., Moore, Finola E., Lobbardi, Riadh, Tenente, Inês M., Ignatius, Myron S., Berman, Jason N., Liwski, Robert S., Houvras, Yariv, Langenau, David M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294527/
https://www.ncbi.nlm.nih.gov/pubmed/25042784
http://dx.doi.org/10.1038/nmeth.3031
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author Tang, Qin
Abdelfattah, Nouran S.
Blackburn, Jessica S.
Moore, John C.
Martinez, Sarah A.
Moore, Finola E.
Lobbardi, Riadh
Tenente, Inês M.
Ignatius, Myron S.
Berman, Jason N.
Liwski, Robert S.
Houvras, Yariv
Langenau, David M.
author_facet Tang, Qin
Abdelfattah, Nouran S.
Blackburn, Jessica S.
Moore, John C.
Martinez, Sarah A.
Moore, Finola E.
Lobbardi, Riadh
Tenente, Inês M.
Ignatius, Myron S.
Berman, Jason N.
Liwski, Robert S.
Houvras, Yariv
Langenau, David M.
author_sort Tang, Qin
collection PubMed
description Cell transplantation into adult zebrafish has lagged behind mouse due to the lack of immune compromised models. Here, we have created homozygous rag2(E450fs) mutant zebrafish that have reduced numbers of functional T and B cells but are viable and fecund. Mutant fish engraft zebrafish muscle, blood stem cells, and cancers. rag2(E450fs) mutant zebrafish are the first immune compromised zebrafish model that permits robust, long-term engraftment of multiple tissues and cancer.
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spelling pubmed-42945272015-02-01 Optimized cell transplantation using adult rag2 mutant zebrafish Tang, Qin Abdelfattah, Nouran S. Blackburn, Jessica S. Moore, John C. Martinez, Sarah A. Moore, Finola E. Lobbardi, Riadh Tenente, Inês M. Ignatius, Myron S. Berman, Jason N. Liwski, Robert S. Houvras, Yariv Langenau, David M. Nat Methods Article Cell transplantation into adult zebrafish has lagged behind mouse due to the lack of immune compromised models. Here, we have created homozygous rag2(E450fs) mutant zebrafish that have reduced numbers of functional T and B cells but are viable and fecund. Mutant fish engraft zebrafish muscle, blood stem cells, and cancers. rag2(E450fs) mutant zebrafish are the first immune compromised zebrafish model that permits robust, long-term engraftment of multiple tissues and cancer. 2014-07-20 2014-08 /pmc/articles/PMC4294527/ /pubmed/25042784 http://dx.doi.org/10.1038/nmeth.3031 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Tang, Qin
Abdelfattah, Nouran S.
Blackburn, Jessica S.
Moore, John C.
Martinez, Sarah A.
Moore, Finola E.
Lobbardi, Riadh
Tenente, Inês M.
Ignatius, Myron S.
Berman, Jason N.
Liwski, Robert S.
Houvras, Yariv
Langenau, David M.
Optimized cell transplantation using adult rag2 mutant zebrafish
title Optimized cell transplantation using adult rag2 mutant zebrafish
title_full Optimized cell transplantation using adult rag2 mutant zebrafish
title_fullStr Optimized cell transplantation using adult rag2 mutant zebrafish
title_full_unstemmed Optimized cell transplantation using adult rag2 mutant zebrafish
title_short Optimized cell transplantation using adult rag2 mutant zebrafish
title_sort optimized cell transplantation using adult rag2 mutant zebrafish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294527/
https://www.ncbi.nlm.nih.gov/pubmed/25042784
http://dx.doi.org/10.1038/nmeth.3031
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