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Modeling Nociception in Zebrafish: A Way Forward for Unbiased Analgesic Discovery

Acute and chronic pain conditions are often debilitating, inflicting severe physiological, emotional and economic costs and affect a large percentage of the global population. However, the development of therapeutic analgesic agents based primarily on targeted drug development has been largely ineff...

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Autores principales: Curtright, Andrew, Rosser, Micaela, Goh, Shamii, Keown, Bailey, Wagner, Erinn, Sharifi, Jasmine, Raible, David W., Dhaka, Ajay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294643/
https://www.ncbi.nlm.nih.gov/pubmed/25587718
http://dx.doi.org/10.1371/journal.pone.0116766
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author Curtright, Andrew
Rosser, Micaela
Goh, Shamii
Keown, Bailey
Wagner, Erinn
Sharifi, Jasmine
Raible, David W.
Dhaka, Ajay
author_facet Curtright, Andrew
Rosser, Micaela
Goh, Shamii
Keown, Bailey
Wagner, Erinn
Sharifi, Jasmine
Raible, David W.
Dhaka, Ajay
author_sort Curtright, Andrew
collection PubMed
description Acute and chronic pain conditions are often debilitating, inflicting severe physiological, emotional and economic costs and affect a large percentage of the global population. However, the development of therapeutic analgesic agents based primarily on targeted drug development has been largely ineffective. An alternative approach to analgesic development would be to develop low cost, high throughput, untargeted animal based behavioral screens that model complex nociceptive behaviors in which to screen for analgesic compounds. Here we describe the development of a behavioral based assay in zebrafish larvae that is effective in identifying small molecule compounds with analgesic properties. In a place aversion assay, which likely utilizes supraspinal neuronal circuitry, individually arrayed zebrafish larvae show temperature-dependent aversion to increasing and decreasing temperatures deviating from rearing temperature. Modeling thermal hyperalgesia, the addition of the noxious inflammatory compound and TRPA1 agonist allyl isothiocyanate sensitized heat aversion and reversed cool aversion leading larvae to avoid rearing temperature in favor of otherwise acutely aversive cooler temperatures. We show that small molecules with known analgesic properties are able to inhibit acute and/or sensitized temperature aversion.
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spelling pubmed-42946432015-01-22 Modeling Nociception in Zebrafish: A Way Forward for Unbiased Analgesic Discovery Curtright, Andrew Rosser, Micaela Goh, Shamii Keown, Bailey Wagner, Erinn Sharifi, Jasmine Raible, David W. Dhaka, Ajay PLoS One Research Article Acute and chronic pain conditions are often debilitating, inflicting severe physiological, emotional and economic costs and affect a large percentage of the global population. However, the development of therapeutic analgesic agents based primarily on targeted drug development has been largely ineffective. An alternative approach to analgesic development would be to develop low cost, high throughput, untargeted animal based behavioral screens that model complex nociceptive behaviors in which to screen for analgesic compounds. Here we describe the development of a behavioral based assay in zebrafish larvae that is effective in identifying small molecule compounds with analgesic properties. In a place aversion assay, which likely utilizes supraspinal neuronal circuitry, individually arrayed zebrafish larvae show temperature-dependent aversion to increasing and decreasing temperatures deviating from rearing temperature. Modeling thermal hyperalgesia, the addition of the noxious inflammatory compound and TRPA1 agonist allyl isothiocyanate sensitized heat aversion and reversed cool aversion leading larvae to avoid rearing temperature in favor of otherwise acutely aversive cooler temperatures. We show that small molecules with known analgesic properties are able to inhibit acute and/or sensitized temperature aversion. Public Library of Science 2015-01-14 /pmc/articles/PMC4294643/ /pubmed/25587718 http://dx.doi.org/10.1371/journal.pone.0116766 Text en © 2015 Curtright et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Curtright, Andrew
Rosser, Micaela
Goh, Shamii
Keown, Bailey
Wagner, Erinn
Sharifi, Jasmine
Raible, David W.
Dhaka, Ajay
Modeling Nociception in Zebrafish: A Way Forward for Unbiased Analgesic Discovery
title Modeling Nociception in Zebrafish: A Way Forward for Unbiased Analgesic Discovery
title_full Modeling Nociception in Zebrafish: A Way Forward for Unbiased Analgesic Discovery
title_fullStr Modeling Nociception in Zebrafish: A Way Forward for Unbiased Analgesic Discovery
title_full_unstemmed Modeling Nociception in Zebrafish: A Way Forward for Unbiased Analgesic Discovery
title_short Modeling Nociception in Zebrafish: A Way Forward for Unbiased Analgesic Discovery
title_sort modeling nociception in zebrafish: a way forward for unbiased analgesic discovery
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294643/
https://www.ncbi.nlm.nih.gov/pubmed/25587718
http://dx.doi.org/10.1371/journal.pone.0116766
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