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Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling
The RAB5 gene family is the best characterised of all human RAB families and is essential for in vitro homotypic fusion of early endosomes. In recent years, the disruption or activation of Rab5 family proteins has been used as a tool to understand growth factor signal transduction in whole animal sy...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294769/ https://www.ncbi.nlm.nih.gov/pubmed/25478908 http://dx.doi.org/10.1016/j.ydbio.2014.11.007 |
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author | Kenyon, Emma J. Campos, Isabel Bull, James C. Williams, P. Huw Stemple, Derek L. Clark, Matthew D. |
author_facet | Kenyon, Emma J. Campos, Isabel Bull, James C. Williams, P. Huw Stemple, Derek L. Clark, Matthew D. |
author_sort | Kenyon, Emma J. |
collection | PubMed |
description | The RAB5 gene family is the best characterised of all human RAB families and is essential for in vitro homotypic fusion of early endosomes. In recent years, the disruption or activation of Rab5 family proteins has been used as a tool to understand growth factor signal transduction in whole animal systems such as Drosophila melanogaster and zebrafish. In this study we have examined the functions for four rab5 genes in zebrafish. Disruption of rab5ab expression by antisense morpholino oligonucleotide (MO) knockdown abolishes nodal signalling in early zebrafish embryos, whereas overexpression of rab5ab mRNA leads to ectopic expression of markers that are normally downstream of nodal signalling. By contrast MO disruption of other zebrafish rab5 genes shows little or no effect on expression of markers of dorsal organiser development. We conclude that rab5ab is essential for nodal signalling and organizer specification in the developing zebrafish embryo. |
format | Online Article Text |
id | pubmed-4294769 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-42947692015-01-21 Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling Kenyon, Emma J. Campos, Isabel Bull, James C. Williams, P. Huw Stemple, Derek L. Clark, Matthew D. Dev Biol Article The RAB5 gene family is the best characterised of all human RAB families and is essential for in vitro homotypic fusion of early endosomes. In recent years, the disruption or activation of Rab5 family proteins has been used as a tool to understand growth factor signal transduction in whole animal systems such as Drosophila melanogaster and zebrafish. In this study we have examined the functions for four rab5 genes in zebrafish. Disruption of rab5ab expression by antisense morpholino oligonucleotide (MO) knockdown abolishes nodal signalling in early zebrafish embryos, whereas overexpression of rab5ab mRNA leads to ectopic expression of markers that are normally downstream of nodal signalling. By contrast MO disruption of other zebrafish rab5 genes shows little or no effect on expression of markers of dorsal organiser development. We conclude that rab5ab is essential for nodal signalling and organizer specification in the developing zebrafish embryo. Elsevier 2015-01-15 /pmc/articles/PMC4294769/ /pubmed/25478908 http://dx.doi.org/10.1016/j.ydbio.2014.11.007 Text en © 2014 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Kenyon, Emma J. Campos, Isabel Bull, James C. Williams, P. Huw Stemple, Derek L. Clark, Matthew D. Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling |
title | Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling |
title_full | Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling |
title_fullStr | Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling |
title_full_unstemmed | Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling |
title_short | Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling |
title_sort | zebrafish rab5 proteins and a role for rab5ab in nodal signalling |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294769/ https://www.ncbi.nlm.nih.gov/pubmed/25478908 http://dx.doi.org/10.1016/j.ydbio.2014.11.007 |
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