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Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling

The RAB5 gene family is the best characterised of all human RAB families and is essential for in vitro homotypic fusion of early endosomes. In recent years, the disruption or activation of Rab5 family proteins has been used as a tool to understand growth factor signal transduction in whole animal sy...

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Autores principales: Kenyon, Emma J., Campos, Isabel, Bull, James C., Williams, P. Huw, Stemple, Derek L., Clark, Matthew D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294769/
https://www.ncbi.nlm.nih.gov/pubmed/25478908
http://dx.doi.org/10.1016/j.ydbio.2014.11.007
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author Kenyon, Emma J.
Campos, Isabel
Bull, James C.
Williams, P. Huw
Stemple, Derek L.
Clark, Matthew D.
author_facet Kenyon, Emma J.
Campos, Isabel
Bull, James C.
Williams, P. Huw
Stemple, Derek L.
Clark, Matthew D.
author_sort Kenyon, Emma J.
collection PubMed
description The RAB5 gene family is the best characterised of all human RAB families and is essential for in vitro homotypic fusion of early endosomes. In recent years, the disruption or activation of Rab5 family proteins has been used as a tool to understand growth factor signal transduction in whole animal systems such as Drosophila melanogaster and zebrafish. In this study we have examined the functions for four rab5 genes in zebrafish. Disruption of rab5ab expression by antisense morpholino oligonucleotide (MO) knockdown abolishes nodal signalling in early zebrafish embryos, whereas overexpression of rab5ab mRNA leads to ectopic expression of markers that are normally downstream of nodal signalling. By contrast MO disruption of other zebrafish rab5 genes shows little or no effect on expression of markers of dorsal organiser development. We conclude that rab5ab is essential for nodal signalling and organizer specification in the developing zebrafish embryo.
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spelling pubmed-42947692015-01-21 Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling Kenyon, Emma J. Campos, Isabel Bull, James C. Williams, P. Huw Stemple, Derek L. Clark, Matthew D. Dev Biol Article The RAB5 gene family is the best characterised of all human RAB families and is essential for in vitro homotypic fusion of early endosomes. In recent years, the disruption or activation of Rab5 family proteins has been used as a tool to understand growth factor signal transduction in whole animal systems such as Drosophila melanogaster and zebrafish. In this study we have examined the functions for four rab5 genes in zebrafish. Disruption of rab5ab expression by antisense morpholino oligonucleotide (MO) knockdown abolishes nodal signalling in early zebrafish embryos, whereas overexpression of rab5ab mRNA leads to ectopic expression of markers that are normally downstream of nodal signalling. By contrast MO disruption of other zebrafish rab5 genes shows little or no effect on expression of markers of dorsal organiser development. We conclude that rab5ab is essential for nodal signalling and organizer specification in the developing zebrafish embryo. Elsevier 2015-01-15 /pmc/articles/PMC4294769/ /pubmed/25478908 http://dx.doi.org/10.1016/j.ydbio.2014.11.007 Text en © 2014 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Kenyon, Emma J.
Campos, Isabel
Bull, James C.
Williams, P. Huw
Stemple, Derek L.
Clark, Matthew D.
Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling
title Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling
title_full Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling
title_fullStr Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling
title_full_unstemmed Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling
title_short Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling
title_sort zebrafish rab5 proteins and a role for rab5ab in nodal signalling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294769/
https://www.ncbi.nlm.nih.gov/pubmed/25478908
http://dx.doi.org/10.1016/j.ydbio.2014.11.007
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