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Assessment of Matrix Metalloproteinases-8 and -9 in Gingival Crevicular Fluid of Smokers and Non-smokers with Chronic Periodontitis Using ELISA
BACKGROUND: Matrix metalloproteinases (MMP)-8 is increased in smokers with chronic periodontitis (CP). The levels of MMP-9 have not been assessed in the periodontal tissues of smokers. Therefore, the aim of this study is to estimate the concentrations of MMP-8 and -9 in the gingival crevicular fluid...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dentmedpub Research and Printing Co
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4295459/ https://www.ncbi.nlm.nih.gov/pubmed/25628487 |
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author | Victor, Dayanand John Subramanian, Sangeetha Gnana, Prakash Ponnudurai Samuel Kolagani, Sai Praneeth |
author_facet | Victor, Dayanand John Subramanian, Sangeetha Gnana, Prakash Ponnudurai Samuel Kolagani, Sai Praneeth |
author_sort | Victor, Dayanand John |
collection | PubMed |
description | BACKGROUND: Matrix metalloproteinases (MMP)-8 is increased in smokers with chronic periodontitis (CP). The levels of MMP-9 have not been assessed in the periodontal tissues of smokers. Therefore, the aim of this study is to estimate the concentrations of MMP-8 and -9 in the gingival crevicular fluid (GCF) of smokers and non-smokers with CP. MATERIALS AND METHODS: Plaque, bleeding on probing, probing pocket depth, and attachment level were assessed in 60 subjects who were equally divided into three groups. Group A consisted of smokers with CP, Group B - non-smokers with CP and Group C - periodontally healthy subjects. The concentrations of MMP-8 and -9 were determined from GCF samples by using enzyme-linked immunosorbent assay. RESULTS: A highly significant difference (P < 0.0001) was found in the MMP-9 concentration between the Groups A (27.56 ± 2.87 ng/ml) and B (17.16 ± 1.74 ng/ml) and between Groups A and C (14.13 ± 2.87 ng/ml). A significant difference (P < 0.002) was also found between the Groups B and C. A highly significant difference (P < 0.0001) in MMP-8 concentration was found between the A (68.96 ± 20.53 ng/ml) and C (7.05 ± 0.75 ng/ml) and also between Groups B (56.97 ± 12.70 ng/ml) and C. The difference in MMP-8 concentration between A and B was not statistically significant. CONCLUSION: Our findings of significantly elevated MMP-9 than MMP-8 from the diseased sites in smokers imply that MMP-9 is more significantly related to the increased periodontal destruction seen among smokers with CP. |
format | Online Article Text |
id | pubmed-4295459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Dentmedpub Research and Printing Co |
record_format | MEDLINE/PubMed |
spelling | pubmed-42954592015-01-27 Assessment of Matrix Metalloproteinases-8 and -9 in Gingival Crevicular Fluid of Smokers and Non-smokers with Chronic Periodontitis Using ELISA Victor, Dayanand John Subramanian, Sangeetha Gnana, Prakash Ponnudurai Samuel Kolagani, Sai Praneeth J Int Oral Health Original Research BACKGROUND: Matrix metalloproteinases (MMP)-8 is increased in smokers with chronic periodontitis (CP). The levels of MMP-9 have not been assessed in the periodontal tissues of smokers. Therefore, the aim of this study is to estimate the concentrations of MMP-8 and -9 in the gingival crevicular fluid (GCF) of smokers and non-smokers with CP. MATERIALS AND METHODS: Plaque, bleeding on probing, probing pocket depth, and attachment level were assessed in 60 subjects who were equally divided into three groups. Group A consisted of smokers with CP, Group B - non-smokers with CP and Group C - periodontally healthy subjects. The concentrations of MMP-8 and -9 were determined from GCF samples by using enzyme-linked immunosorbent assay. RESULTS: A highly significant difference (P < 0.0001) was found in the MMP-9 concentration between the Groups A (27.56 ± 2.87 ng/ml) and B (17.16 ± 1.74 ng/ml) and between Groups A and C (14.13 ± 2.87 ng/ml). A significant difference (P < 0.002) was also found between the Groups B and C. A highly significant difference (P < 0.0001) in MMP-8 concentration was found between the A (68.96 ± 20.53 ng/ml) and C (7.05 ± 0.75 ng/ml) and also between Groups B (56.97 ± 12.70 ng/ml) and C. The difference in MMP-8 concentration between A and B was not statistically significant. CONCLUSION: Our findings of significantly elevated MMP-9 than MMP-8 from the diseased sites in smokers imply that MMP-9 is more significantly related to the increased periodontal destruction seen among smokers with CP. Dentmedpub Research and Printing Co 2014 /pmc/articles/PMC4295459/ /pubmed/25628487 Text en Copyright: © J. Int Oral Health http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Victor, Dayanand John Subramanian, Sangeetha Gnana, Prakash Ponnudurai Samuel Kolagani, Sai Praneeth Assessment of Matrix Metalloproteinases-8 and -9 in Gingival Crevicular Fluid of Smokers and Non-smokers with Chronic Periodontitis Using ELISA |
title | Assessment of Matrix Metalloproteinases-8 and -9 in Gingival Crevicular Fluid of Smokers and Non-smokers with Chronic Periodontitis Using ELISA |
title_full | Assessment of Matrix Metalloproteinases-8 and -9 in Gingival Crevicular Fluid of Smokers and Non-smokers with Chronic Periodontitis Using ELISA |
title_fullStr | Assessment of Matrix Metalloproteinases-8 and -9 in Gingival Crevicular Fluid of Smokers and Non-smokers with Chronic Periodontitis Using ELISA |
title_full_unstemmed | Assessment of Matrix Metalloproteinases-8 and -9 in Gingival Crevicular Fluid of Smokers and Non-smokers with Chronic Periodontitis Using ELISA |
title_short | Assessment of Matrix Metalloproteinases-8 and -9 in Gingival Crevicular Fluid of Smokers and Non-smokers with Chronic Periodontitis Using ELISA |
title_sort | assessment of matrix metalloproteinases-8 and -9 in gingival crevicular fluid of smokers and non-smokers with chronic periodontitis using elisa |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4295459/ https://www.ncbi.nlm.nih.gov/pubmed/25628487 |
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