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Biomimetic synthesis of struvite with biogenic morphology and implication for pathological biomineralization

Recent studies have found that certain urinary proteins can efficiently inhibit stone formation. These discoveries are significant for developing effective therapies for stone disease, but the inhibition mechanism of crystallization remains elusive. In the present study, polyaspartic acid (PASP) was...

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Autores principales: Li, Han, Yao, Qi-Zhi, Wang, Yu-Ying, Li, Yi-Liang, Zhou, Gen-Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296295/
https://www.ncbi.nlm.nih.gov/pubmed/25591814
http://dx.doi.org/10.1038/srep07718
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author Li, Han
Yao, Qi-Zhi
Wang, Yu-Ying
Li, Yi-Liang
Zhou, Gen-Tao
author_facet Li, Han
Yao, Qi-Zhi
Wang, Yu-Ying
Li, Yi-Liang
Zhou, Gen-Tao
author_sort Li, Han
collection PubMed
description Recent studies have found that certain urinary proteins can efficiently inhibit stone formation. These discoveries are significant for developing effective therapies for stone disease, but the inhibition mechanism of crystallization remains elusive. In the present study, polyaspartic acid (PASP) was employed as a model peptide to investigate the effect of urinary proteins on the crystallization and morphological evolution of struvite. The results demonstrate that selective adsorption/binding of PASP onto the {010} and {101} faces of struvite crystals results in arrowhead-shaped morphology, which further evolves into X-shaped and unusual tabular structures with time. Noticeably, these morphologies are reminiscent of biogenic struvite morphology. Concentration-dependent experiments show that PASP can inhibit struvite growth and the inhibitory capacity increases with increasing PASP concentration, whereas aspartic acid monomers do not show a significant effect. Considering that PASP is a structural and functional analogue of the subdomains of aspartic acid-rich proteins, our results reveal that aspartic acid-rich proteins play a key role in regulating biogenic struvite morphology, and aspartic acid residues contribute to the inhibitory capacity of urinary proteins. The potential implications of PASP for developing therapeutic agents for urinary stone disease is also discussed.
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spelling pubmed-42962952015-01-16 Biomimetic synthesis of struvite with biogenic morphology and implication for pathological biomineralization Li, Han Yao, Qi-Zhi Wang, Yu-Ying Li, Yi-Liang Zhou, Gen-Tao Sci Rep Article Recent studies have found that certain urinary proteins can efficiently inhibit stone formation. These discoveries are significant for developing effective therapies for stone disease, but the inhibition mechanism of crystallization remains elusive. In the present study, polyaspartic acid (PASP) was employed as a model peptide to investigate the effect of urinary proteins on the crystallization and morphological evolution of struvite. The results demonstrate that selective adsorption/binding of PASP onto the {010} and {101} faces of struvite crystals results in arrowhead-shaped morphology, which further evolves into X-shaped and unusual tabular structures with time. Noticeably, these morphologies are reminiscent of biogenic struvite morphology. Concentration-dependent experiments show that PASP can inhibit struvite growth and the inhibitory capacity increases with increasing PASP concentration, whereas aspartic acid monomers do not show a significant effect. Considering that PASP is a structural and functional analogue of the subdomains of aspartic acid-rich proteins, our results reveal that aspartic acid-rich proteins play a key role in regulating biogenic struvite morphology, and aspartic acid residues contribute to the inhibitory capacity of urinary proteins. The potential implications of PASP for developing therapeutic agents for urinary stone disease is also discussed. Nature Publishing Group 2015-01-16 /pmc/articles/PMC4296295/ /pubmed/25591814 http://dx.doi.org/10.1038/srep07718 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Article
Li, Han
Yao, Qi-Zhi
Wang, Yu-Ying
Li, Yi-Liang
Zhou, Gen-Tao
Biomimetic synthesis of struvite with biogenic morphology and implication for pathological biomineralization
title Biomimetic synthesis of struvite with biogenic morphology and implication for pathological biomineralization
title_full Biomimetic synthesis of struvite with biogenic morphology and implication for pathological biomineralization
title_fullStr Biomimetic synthesis of struvite with biogenic morphology and implication for pathological biomineralization
title_full_unstemmed Biomimetic synthesis of struvite with biogenic morphology and implication for pathological biomineralization
title_short Biomimetic synthesis of struvite with biogenic morphology and implication for pathological biomineralization
title_sort biomimetic synthesis of struvite with biogenic morphology and implication for pathological biomineralization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296295/
https://www.ncbi.nlm.nih.gov/pubmed/25591814
http://dx.doi.org/10.1038/srep07718
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