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Interferon-γ inhibits group B Streptococcus survival within human endothelial cells

Endothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs...

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Autores principales: Lione, Viviane de Oliveira Freitas, dos Santos, Michelle Hanthequeste Bittencourt, de Oliveira, Jessica Silva Santos, Mattos-Guaraldi, Ana Luiza, Nagao, Prescilla Emy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296500/
https://www.ncbi.nlm.nih.gov/pubmed/25410999
http://dx.doi.org/10.1590/0074-0276140201
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author Lione, Viviane de Oliveira Freitas
dos Santos, Michelle Hanthequeste Bittencourt
de Oliveira, Jessica Silva Santos
Mattos-Guaraldi, Ana Luiza
Nagao, Prescilla Emy
author_facet Lione, Viviane de Oliveira Freitas
dos Santos, Michelle Hanthequeste Bittencourt
de Oliveira, Jessica Silva Santos
Mattos-Guaraldi, Ana Luiza
Nagao, Prescilla Emy
author_sort Lione, Viviane de Oliveira Freitas
collection PubMed
description Endothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs against GBS has not been studied. We report that the viability of intracellular bacteria was reduced in HUVECs activated by IFN-γ. Enhanced fusion of lysosomes with bacteria-containing vacuoles was observed by acid phosphatase and the colocalisation of Rab-5, Rab-7 and lysosomal-associated membrane protein-1 with GBS in IFN-γ-activated HUVECs. IFN-γ resulted in an enhancement of the phagosome maturation process in HUVECs, improving the capacity to control the intracellular survival of GBS.
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spelling pubmed-42965002015-01-23 Interferon-γ inhibits group B Streptococcus survival within human endothelial cells Lione, Viviane de Oliveira Freitas dos Santos, Michelle Hanthequeste Bittencourt de Oliveira, Jessica Silva Santos Mattos-Guaraldi, Ana Luiza Nagao, Prescilla Emy Mem Inst Oswaldo Cruz Short Communication Endothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs against GBS has not been studied. We report that the viability of intracellular bacteria was reduced in HUVECs activated by IFN-γ. Enhanced fusion of lysosomes with bacteria-containing vacuoles was observed by acid phosphatase and the colocalisation of Rab-5, Rab-7 and lysosomal-associated membrane protein-1 with GBS in IFN-γ-activated HUVECs. IFN-γ resulted in an enhancement of the phagosome maturation process in HUVECs, improving the capacity to control the intracellular survival of GBS. Instituto Oswaldo Cruz, Ministério da Saúde 2014-11 /pmc/articles/PMC4296500/ /pubmed/25410999 http://dx.doi.org/10.1590/0074-0276140201 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Lione, Viviane de Oliveira Freitas
dos Santos, Michelle Hanthequeste Bittencourt
de Oliveira, Jessica Silva Santos
Mattos-Guaraldi, Ana Luiza
Nagao, Prescilla Emy
Interferon-γ inhibits group B Streptococcus survival within human endothelial cells
title Interferon-γ inhibits group B Streptococcus survival within human endothelial cells
title_full Interferon-γ inhibits group B Streptococcus survival within human endothelial cells
title_fullStr Interferon-γ inhibits group B Streptococcus survival within human endothelial cells
title_full_unstemmed Interferon-γ inhibits group B Streptococcus survival within human endothelial cells
title_short Interferon-γ inhibits group B Streptococcus survival within human endothelial cells
title_sort interferon-γ inhibits group b streptococcus survival within human endothelial cells
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296500/
https://www.ncbi.nlm.nih.gov/pubmed/25410999
http://dx.doi.org/10.1590/0074-0276140201
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