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Interferon-γ inhibits group B Streptococcus survival within human endothelial cells
Endothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296500/ https://www.ncbi.nlm.nih.gov/pubmed/25410999 http://dx.doi.org/10.1590/0074-0276140201 |
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author | Lione, Viviane de Oliveira Freitas dos Santos, Michelle Hanthequeste Bittencourt de Oliveira, Jessica Silva Santos Mattos-Guaraldi, Ana Luiza Nagao, Prescilla Emy |
author_facet | Lione, Viviane de Oliveira Freitas dos Santos, Michelle Hanthequeste Bittencourt de Oliveira, Jessica Silva Santos Mattos-Guaraldi, Ana Luiza Nagao, Prescilla Emy |
author_sort | Lione, Viviane de Oliveira Freitas |
collection | PubMed |
description | Endothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs against GBS has not been studied. We report that the viability of intracellular bacteria was reduced in HUVECs activated by IFN-γ. Enhanced fusion of lysosomes with bacteria-containing vacuoles was observed by acid phosphatase and the colocalisation of Rab-5, Rab-7 and lysosomal-associated membrane protein-1 with GBS in IFN-γ-activated HUVECs. IFN-γ resulted in an enhancement of the phagosome maturation process in HUVECs, improving the capacity to control the intracellular survival of GBS. |
format | Online Article Text |
id | pubmed-4296500 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-42965002015-01-23 Interferon-γ inhibits group B Streptococcus survival within human endothelial cells Lione, Viviane de Oliveira Freitas dos Santos, Michelle Hanthequeste Bittencourt de Oliveira, Jessica Silva Santos Mattos-Guaraldi, Ana Luiza Nagao, Prescilla Emy Mem Inst Oswaldo Cruz Short Communication Endothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs against GBS has not been studied. We report that the viability of intracellular bacteria was reduced in HUVECs activated by IFN-γ. Enhanced fusion of lysosomes with bacteria-containing vacuoles was observed by acid phosphatase and the colocalisation of Rab-5, Rab-7 and lysosomal-associated membrane protein-1 with GBS in IFN-γ-activated HUVECs. IFN-γ resulted in an enhancement of the phagosome maturation process in HUVECs, improving the capacity to control the intracellular survival of GBS. Instituto Oswaldo Cruz, Ministério da Saúde 2014-11 /pmc/articles/PMC4296500/ /pubmed/25410999 http://dx.doi.org/10.1590/0074-0276140201 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Lione, Viviane de Oliveira Freitas dos Santos, Michelle Hanthequeste Bittencourt de Oliveira, Jessica Silva Santos Mattos-Guaraldi, Ana Luiza Nagao, Prescilla Emy Interferon-γ inhibits group B Streptococcus survival within human endothelial cells |
title | Interferon-γ inhibits group B Streptococcus survival
within human endothelial cells |
title_full | Interferon-γ inhibits group B Streptococcus survival
within human endothelial cells |
title_fullStr | Interferon-γ inhibits group B Streptococcus survival
within human endothelial cells |
title_full_unstemmed | Interferon-γ inhibits group B Streptococcus survival
within human endothelial cells |
title_short | Interferon-γ inhibits group B Streptococcus survival
within human endothelial cells |
title_sort | interferon-γ inhibits group b streptococcus survival
within human endothelial cells |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296500/ https://www.ncbi.nlm.nih.gov/pubmed/25410999 http://dx.doi.org/10.1590/0074-0276140201 |
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