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EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES

INTRODUCTION: Methicillin-Resistant Staphylococcus aureus (MRSA) presenting reduced susceptibility to vancomycin has been associated to therapeutic failure. Some methods used by clinical laboratories may not be sufficiently accurate to detect this phenotype, compromising results and the outcome of t...

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Autores principales: Rossatto, Fernanda Cristina Possamai, Proença, Letícia Auler, Becker, Ana Paula, Silveira, Alessandro Conrado de Oliveira, Caierão, Juliana, D'azevedo, Pedro Alves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296864/
https://www.ncbi.nlm.nih.gov/pubmed/25351538
http://dx.doi.org/10.1590/S0036-46652014000600002
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author Rossatto, Fernanda Cristina Possamai
Proença, Letícia Auler
Becker, Ana Paula
Silveira, Alessandro Conrado de Oliveira
Caierão, Juliana
D'azevedo, Pedro Alves
author_facet Rossatto, Fernanda Cristina Possamai
Proença, Letícia Auler
Becker, Ana Paula
Silveira, Alessandro Conrado de Oliveira
Caierão, Juliana
D'azevedo, Pedro Alves
author_sort Rossatto, Fernanda Cristina Possamai
collection PubMed
description INTRODUCTION: Methicillin-Resistant Staphylococcus aureus (MRSA) presenting reduced susceptibility to vancomycin has been associated to therapeutic failure. Some methods used by clinical laboratories may not be sufficiently accurate to detect this phenotype, compromising results and the outcome of the patient. OBJECTIVES: To evaluate the performance of methods in the detection of vancomycin MIC values among clinical isolates of MRSA. MATERIAL AND METHODS: The Vancomycin Minimal Inhibitory Concentration was determined for 75 MRSA isolates from inpatients of Mãe de Deus Hospital, Porto Alegre, Brazil. The broth microdilution (BM) was used as the gold-standard technique, as well as the following methods: E-test(®) strips (BioMérieux), M.I.C.E(®) strips (Oxoid), PROBAC(®) commercial panel and the automated system MicroScan(®) (Siemens). Besides, the agar screening test was carried out with 3 µg/mL of vancomycin. RESULTS: All isolates presented MIC ≤ 2 µg/mL for BM. E-test(®) had higher concordance (40%) in terms of global agreement with the gold standard, and there was not statistical difference among E-test(®) and broth microdilution results. PROBAC(®) panels presented MICs, in general, lower than the gold-standard panels (58.66% major errors), while M.I.C.E.(®) MICs were higher (67.99% minor errors). CONCLUSIONS: For the population of MRSA in question, E-test(®) presented the best performance, although with a heterogeneous accuracy, depending on MIC values.
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spelling pubmed-42968642015-01-20 EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES Rossatto, Fernanda Cristina Possamai Proença, Letícia Auler Becker, Ana Paula Silveira, Alessandro Conrado de Oliveira Caierão, Juliana D'azevedo, Pedro Alves Rev Inst Med Trop Sao Paulo Microbiology INTRODUCTION: Methicillin-Resistant Staphylococcus aureus (MRSA) presenting reduced susceptibility to vancomycin has been associated to therapeutic failure. Some methods used by clinical laboratories may not be sufficiently accurate to detect this phenotype, compromising results and the outcome of the patient. OBJECTIVES: To evaluate the performance of methods in the detection of vancomycin MIC values among clinical isolates of MRSA. MATERIAL AND METHODS: The Vancomycin Minimal Inhibitory Concentration was determined for 75 MRSA isolates from inpatients of Mãe de Deus Hospital, Porto Alegre, Brazil. The broth microdilution (BM) was used as the gold-standard technique, as well as the following methods: E-test(®) strips (BioMérieux), M.I.C.E(®) strips (Oxoid), PROBAC(®) commercial panel and the automated system MicroScan(®) (Siemens). Besides, the agar screening test was carried out with 3 µg/mL of vancomycin. RESULTS: All isolates presented MIC ≤ 2 µg/mL for BM. E-test(®) had higher concordance (40%) in terms of global agreement with the gold standard, and there was not statistical difference among E-test(®) and broth microdilution results. PROBAC(®) panels presented MICs, in general, lower than the gold-standard panels (58.66% major errors), while M.I.C.E.(®) MICs were higher (67.99% minor errors). CONCLUSIONS: For the population of MRSA in question, E-test(®) presented the best performance, although with a heterogeneous accuracy, depending on MIC values. Instituto de Medicina Tropical 2014 /pmc/articles/PMC4296864/ /pubmed/25351538 http://dx.doi.org/10.1590/S0036-46652014000600002 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Microbiology
Rossatto, Fernanda Cristina Possamai
Proença, Letícia Auler
Becker, Ana Paula
Silveira, Alessandro Conrado de Oliveira
Caierão, Juliana
D'azevedo, Pedro Alves
EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES
title EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES
title_full EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES
title_fullStr EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES
title_full_unstemmed EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES
title_short EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES
title_sort evaluation of methods in detecting vancomycin mic among mrsa isolates and the changes in accuracy related to different mic values
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296864/
https://www.ncbi.nlm.nih.gov/pubmed/25351538
http://dx.doi.org/10.1590/S0036-46652014000600002
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