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EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES
INTRODUCTION: Methicillin-Resistant Staphylococcus aureus (MRSA) presenting reduced susceptibility to vancomycin has been associated to therapeutic failure. Some methods used by clinical laboratories may not be sufficiently accurate to detect this phenotype, compromising results and the outcome of t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Instituto de Medicina Tropical
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296864/ https://www.ncbi.nlm.nih.gov/pubmed/25351538 http://dx.doi.org/10.1590/S0036-46652014000600002 |
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author | Rossatto, Fernanda Cristina Possamai Proença, Letícia Auler Becker, Ana Paula Silveira, Alessandro Conrado de Oliveira Caierão, Juliana D'azevedo, Pedro Alves |
author_facet | Rossatto, Fernanda Cristina Possamai Proença, Letícia Auler Becker, Ana Paula Silveira, Alessandro Conrado de Oliveira Caierão, Juliana D'azevedo, Pedro Alves |
author_sort | Rossatto, Fernanda Cristina Possamai |
collection | PubMed |
description | INTRODUCTION: Methicillin-Resistant Staphylococcus aureus (MRSA) presenting reduced susceptibility to vancomycin has been associated to therapeutic failure. Some methods used by clinical laboratories may not be sufficiently accurate to detect this phenotype, compromising results and the outcome of the patient. OBJECTIVES: To evaluate the performance of methods in the detection of vancomycin MIC values among clinical isolates of MRSA. MATERIAL AND METHODS: The Vancomycin Minimal Inhibitory Concentration was determined for 75 MRSA isolates from inpatients of Mãe de Deus Hospital, Porto Alegre, Brazil. The broth microdilution (BM) was used as the gold-standard technique, as well as the following methods: E-test(®) strips (BioMérieux), M.I.C.E(®) strips (Oxoid), PROBAC(®) commercial panel and the automated system MicroScan(®) (Siemens). Besides, the agar screening test was carried out with 3 µg/mL of vancomycin. RESULTS: All isolates presented MIC ≤ 2 µg/mL for BM. E-test(®) had higher concordance (40%) in terms of global agreement with the gold standard, and there was not statistical difference among E-test(®) and broth microdilution results. PROBAC(®) panels presented MICs, in general, lower than the gold-standard panels (58.66% major errors), while M.I.C.E.(®) MICs were higher (67.99% minor errors). CONCLUSIONS: For the population of MRSA in question, E-test(®) presented the best performance, although with a heterogeneous accuracy, depending on MIC values. |
format | Online Article Text |
id | pubmed-4296864 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Instituto de Medicina Tropical |
record_format | MEDLINE/PubMed |
spelling | pubmed-42968642015-01-20 EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES Rossatto, Fernanda Cristina Possamai Proença, Letícia Auler Becker, Ana Paula Silveira, Alessandro Conrado de Oliveira Caierão, Juliana D'azevedo, Pedro Alves Rev Inst Med Trop Sao Paulo Microbiology INTRODUCTION: Methicillin-Resistant Staphylococcus aureus (MRSA) presenting reduced susceptibility to vancomycin has been associated to therapeutic failure. Some methods used by clinical laboratories may not be sufficiently accurate to detect this phenotype, compromising results and the outcome of the patient. OBJECTIVES: To evaluate the performance of methods in the detection of vancomycin MIC values among clinical isolates of MRSA. MATERIAL AND METHODS: The Vancomycin Minimal Inhibitory Concentration was determined for 75 MRSA isolates from inpatients of Mãe de Deus Hospital, Porto Alegre, Brazil. The broth microdilution (BM) was used as the gold-standard technique, as well as the following methods: E-test(®) strips (BioMérieux), M.I.C.E(®) strips (Oxoid), PROBAC(®) commercial panel and the automated system MicroScan(®) (Siemens). Besides, the agar screening test was carried out with 3 µg/mL of vancomycin. RESULTS: All isolates presented MIC ≤ 2 µg/mL for BM. E-test(®) had higher concordance (40%) in terms of global agreement with the gold standard, and there was not statistical difference among E-test(®) and broth microdilution results. PROBAC(®) panels presented MICs, in general, lower than the gold-standard panels (58.66% major errors), while M.I.C.E.(®) MICs were higher (67.99% minor errors). CONCLUSIONS: For the population of MRSA in question, E-test(®) presented the best performance, although with a heterogeneous accuracy, depending on MIC values. Instituto de Medicina Tropical 2014 /pmc/articles/PMC4296864/ /pubmed/25351538 http://dx.doi.org/10.1590/S0036-46652014000600002 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Microbiology Rossatto, Fernanda Cristina Possamai Proença, Letícia Auler Becker, Ana Paula Silveira, Alessandro Conrado de Oliveira Caierão, Juliana D'azevedo, Pedro Alves EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES |
title | EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG
MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC
VALUES |
title_full | EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG
MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC
VALUES |
title_fullStr | EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG
MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC
VALUES |
title_full_unstemmed | EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG
MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC
VALUES |
title_short | EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG
MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC
VALUES |
title_sort | evaluation of methods in detecting vancomycin mic among
mrsa isolates and the changes in accuracy related to different mic
values |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296864/ https://www.ncbi.nlm.nih.gov/pubmed/25351538 http://dx.doi.org/10.1590/S0036-46652014000600002 |
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