USE OF MUELLER-HINTON BROTH AND AGAR IN THE GERM TUBE TEST

Candida albicans is often isolated from clinical samples, thus its presumptive differentiation from other species of the same genus can be based on its ability to form the germ tube in human serum. Nevertheless, there are two other species that share this characteristic: C. dubliniensis and C. afric...

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Autores principales: Mattei, Antonella Souza, Alves, Sydney Hartz, Severo, Cecília Bittencourt, Guazzelli, Luciana da Silva, Oliveira, Flávio de Mattos, Severo, Luiz Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2014
Materias:
Mycology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296867/
https://www.ncbi.nlm.nih.gov/pubmed/25351541
http://dx.doi.org/10.1590/S0036-46652014000600005
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author Mattei, Antonella Souza
Alves, Sydney Hartz
Severo, Cecília Bittencourt
Guazzelli, Luciana da Silva
Oliveira, Flávio de Mattos
Severo, Luiz Carlos
author_facet Mattei, Antonella Souza
Alves, Sydney Hartz
Severo, Cecília Bittencourt
Guazzelli, Luciana da Silva
Oliveira, Flávio de Mattos
Severo, Luiz Carlos
author_sort Mattei, Antonella Souza
collection PubMed
description Candida albicans is often isolated from clinical samples, thus its presumptive differentiation from other species of the same genus can be based on its ability to form the germ tube in human serum. Nevertheless, there are two other species that share this characteristic: C. dubliniensis and C. africana. The aim of this study was to compare four different substrates to perform the germ tube (GT) test. The Candida spp. isolates were identified using a manual system (135 C. albicans, 24 C. tropicalis and one C. dubliniensis). The germ tube test was performed with fresh, previously frozen serum and Mueller-Hinton (MH) broth and agar. GT was observed in 96% (130/136) of the isolates through the fresh serum technique, 94% (128/136) through previously frozen serum, 92% (125/136) in MH agar, and 90% (122/136) in MH broth. The sensitivity of each test was higher than 90%, with 100% specificity. Both the MH agar and broth were able to identify the true positives, and false positives were not found. However, some C. albicans isolates were not identified. MH agar and broth may be used in laboratory for the rapid presumptive identification of C. albicans, as an alternative method for germ tube test.
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spelling pubmed-42968672015-01-20 USE OF MUELLER-HINTON BROTH AND AGAR IN THE GERM TUBE TEST Mattei, Antonella Souza Alves, Sydney Hartz Severo, Cecília Bittencourt Guazzelli, Luciana da Silva Oliveira, Flávio de Mattos Severo, Luiz Carlos Rev Inst Med Trop Sao Paulo Mycology Candida albicans is often isolated from clinical samples, thus its presumptive differentiation from other species of the same genus can be based on its ability to form the germ tube in human serum. Nevertheless, there are two other species that share this characteristic: C. dubliniensis and C. africana. The aim of this study was to compare four different substrates to perform the germ tube (GT) test. The Candida spp. isolates were identified using a manual system (135 C. albicans, 24 C. tropicalis and one C. dubliniensis). The germ tube test was performed with fresh, previously frozen serum and Mueller-Hinton (MH) broth and agar. GT was observed in 96% (130/136) of the isolates through the fresh serum technique, 94% (128/136) through previously frozen serum, 92% (125/136) in MH agar, and 90% (122/136) in MH broth. The sensitivity of each test was higher than 90%, with 100% specificity. Both the MH agar and broth were able to identify the true positives, and false positives were not found. However, some C. albicans isolates were not identified. MH agar and broth may be used in laboratory for the rapid presumptive identification of C. albicans, as an alternative method for germ tube test. Instituto de Medicina Tropical 2014 /pmc/articles/PMC4296867/ /pubmed/25351541 http://dx.doi.org/10.1590/S0036-46652014000600005 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Mycology
Mattei, Antonella Souza
Alves, Sydney Hartz
Severo, Cecília Bittencourt
Guazzelli, Luciana da Silva
Oliveira, Flávio de Mattos
Severo, Luiz Carlos
USE OF MUELLER-HINTON BROTH AND AGAR IN THE GERM TUBE TEST
title USE OF MUELLER-HINTON BROTH AND AGAR IN THE GERM TUBE TEST
title_full USE OF MUELLER-HINTON BROTH AND AGAR IN THE GERM TUBE TEST
title_fullStr USE OF MUELLER-HINTON BROTH AND AGAR IN THE GERM TUBE TEST
title_full_unstemmed USE OF MUELLER-HINTON BROTH AND AGAR IN THE GERM TUBE TEST
title_short USE OF MUELLER-HINTON BROTH AND AGAR IN THE GERM TUBE TEST
title_sort use of mueller-hinton broth and agar in the germ tube test
topic Mycology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296867/
https://www.ncbi.nlm.nih.gov/pubmed/25351541
http://dx.doi.org/10.1590/S0036-46652014000600005
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