Directed evolution of APEX2 for electron microscopy and proteomics

APEX is an engineered peroxidase that functions both as an electron microscopy tag, and as a promiscuous labeling enzyme for live-cell proteomics. Because the limited sensitivity of APEX precludes applications requiring low APEX expression, we used yeast display evolution to improve its catalytic ef...

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Detalles Bibliográficos
Autores principales: Lam, Stephanie S., Martell, Jeffrey D., Kamer, Kimberli J., Deerinck, Thomas J., Ellisman, Mark H., Mootha, Vamsi K., Ting, Alice Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296904/
https://www.ncbi.nlm.nih.gov/pubmed/25419960
http://dx.doi.org/10.1038/nmeth.3179

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296904/
https://www.ncbi.nlm.nih.gov/pubmed/25419960
http://dx.doi.org/10.1038/nmeth.3179

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