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Development of a Spring-Loaded Impact Device to Deliver Injurious Mechanical Impacts to the Articular Cartilage Surface

OBJECTIVE: Traumatic impacts on the articular joint surface in vitro are known to lead to degeneration of the cartilage. The main objective of this study was to develop a spring-loaded impact device that can be used to deliver traumatic impacts of consistent magnitude and rate and to find whether im...

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Autores principales: Alexander, Peter G., Song, Yingjie, Taboas, Juan M., Chen, Faye H., Melvin, Gary M., Manner, Paul A., Tuan, Rocky S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4297114/
https://www.ncbi.nlm.nih.gov/pubmed/26069650
http://dx.doi.org/10.1177/1947603512455195
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author Alexander, Peter G.
Song, Yingjie
Taboas, Juan M.
Chen, Faye H.
Melvin, Gary M.
Manner, Paul A.
Tuan, Rocky S.
author_facet Alexander, Peter G.
Song, Yingjie
Taboas, Juan M.
Chen, Faye H.
Melvin, Gary M.
Manner, Paul A.
Tuan, Rocky S.
author_sort Alexander, Peter G.
collection PubMed
description OBJECTIVE: Traumatic impacts on the articular joint surface in vitro are known to lead to degeneration of the cartilage. The main objective of this study was to develop a spring-loaded impact device that can be used to deliver traumatic impacts of consistent magnitude and rate and to find whether impacts cause catabolic activities in articular cartilage consistent with other previously reported impact models and correlated with the development of osteoarthritic lesions. In developing the spring-loaded impactor, the operating hypothesis is that a single supraphysiologic impact to articular cartilage in vitro can affect cartilage integrity, cell viability, sulfated glycosaminoglycan and inflammatory mediator release in a dose-dependent manner. DESIGN: Impacts of increasing force are delivered to adult bovine articular cartilage explants in confined compression. Impact parameters are correlated with tissue damage, cell viability, matrix and inflammatory mediator release, and gene expression 24 hours postimpact. RESULTS: Nitric oxide release is first detected after 7.7 MPa impacts, whereas cell death, glycosaminoglycan release, and prostaglandin E2 release are first detected at 17 MPa. Catabolic markers increase linearly to maximal levels after ≥36 MPa impacts. CONCLUSIONS: A single supraphysiologic impact negatively affects cartilage integrity, cell viability, and GAG release in a dose-dependent manner. Our findings showed that 7 to 17 MPa impacts can induce cell death and catabolism without compromising the articular surface, whereas a 17 MPa impact is sufficient to induce increases in most common catabolic markers of osteoarthritic degeneration.
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spelling pubmed-42971142015-06-11 Development of a Spring-Loaded Impact Device to Deliver Injurious Mechanical Impacts to the Articular Cartilage Surface Alexander, Peter G. Song, Yingjie Taboas, Juan M. Chen, Faye H. Melvin, Gary M. Manner, Paul A. Tuan, Rocky S. Cartilage Article OBJECTIVE: Traumatic impacts on the articular joint surface in vitro are known to lead to degeneration of the cartilage. The main objective of this study was to develop a spring-loaded impact device that can be used to deliver traumatic impacts of consistent magnitude and rate and to find whether impacts cause catabolic activities in articular cartilage consistent with other previously reported impact models and correlated with the development of osteoarthritic lesions. In developing the spring-loaded impactor, the operating hypothesis is that a single supraphysiologic impact to articular cartilage in vitro can affect cartilage integrity, cell viability, sulfated glycosaminoglycan and inflammatory mediator release in a dose-dependent manner. DESIGN: Impacts of increasing force are delivered to adult bovine articular cartilage explants in confined compression. Impact parameters are correlated with tissue damage, cell viability, matrix and inflammatory mediator release, and gene expression 24 hours postimpact. RESULTS: Nitric oxide release is first detected after 7.7 MPa impacts, whereas cell death, glycosaminoglycan release, and prostaglandin E2 release are first detected at 17 MPa. Catabolic markers increase linearly to maximal levels after ≥36 MPa impacts. CONCLUSIONS: A single supraphysiologic impact negatively affects cartilage integrity, cell viability, and GAG release in a dose-dependent manner. Our findings showed that 7 to 17 MPa impacts can induce cell death and catabolism without compromising the articular surface, whereas a 17 MPa impact is sufficient to induce increases in most common catabolic markers of osteoarthritic degeneration. SAGE Publications 2013-01 /pmc/articles/PMC4297114/ /pubmed/26069650 http://dx.doi.org/10.1177/1947603512455195 Text en © The Author(s) 2013
spellingShingle Article
Alexander, Peter G.
Song, Yingjie
Taboas, Juan M.
Chen, Faye H.
Melvin, Gary M.
Manner, Paul A.
Tuan, Rocky S.
Development of a Spring-Loaded Impact Device to Deliver Injurious Mechanical Impacts to the Articular Cartilage Surface
title Development of a Spring-Loaded Impact Device to Deliver Injurious Mechanical Impacts to the Articular Cartilage Surface
title_full Development of a Spring-Loaded Impact Device to Deliver Injurious Mechanical Impacts to the Articular Cartilage Surface
title_fullStr Development of a Spring-Loaded Impact Device to Deliver Injurious Mechanical Impacts to the Articular Cartilage Surface
title_full_unstemmed Development of a Spring-Loaded Impact Device to Deliver Injurious Mechanical Impacts to the Articular Cartilage Surface
title_short Development of a Spring-Loaded Impact Device to Deliver Injurious Mechanical Impacts to the Articular Cartilage Surface
title_sort development of a spring-loaded impact device to deliver injurious mechanical impacts to the articular cartilage surface
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4297114/
https://www.ncbi.nlm.nih.gov/pubmed/26069650
http://dx.doi.org/10.1177/1947603512455195
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