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Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine
BACKGROUND: Encephalomyocarditis virus (EMCV) can cause myocarditis, respiratory failure, reproductive failure, and sudden death in pre-weaned piglets, which has been isolated in China. EMCV VP1 protein was one of the most important structural proteins and played an important role in the protective...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4297377/ https://www.ncbi.nlm.nih.gov/pubmed/25547933 http://dx.doi.org/10.1186/s12985-014-0226-8 |
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author | Bai, Juan Chen, Xinhui Jiang, Kangfu Zeshan, Basit Jiang, Ping |
author_facet | Bai, Juan Chen, Xinhui Jiang, Kangfu Zeshan, Basit Jiang, Ping |
author_sort | Bai, Juan |
collection | PubMed |
description | BACKGROUND: Encephalomyocarditis virus (EMCV) can cause myocarditis, respiratory failure, reproductive failure, and sudden death in pre-weaned piglets, which has been isolated in China. EMCV VP1 protein was one of the most important structural proteins and played an important role in the protective immunity. In this study, 10 monoclonal antibodies (McAbs) against EMCV VP1 were screened and identified. RESULTS: Epitope mapping results indicated that McAbs (6E11, 7A7, 7C9) specifically recognized the linear epitopes V(2)ENAEK(7), McAbs (1D1, 2A2, 5A1, 5A11, 5G1) recognized the epitope F(19)VAQPVY(25), and McAbs 1G8 and 3A9 recognized P(42)IGAFTVK(49). Protein sequence alignment of VP1 with 16 EMCV isolates indicated that the epitope F(19)VAQPVY(25) was conserved in all the reference strains. The epitopes P(42)IGAFTVK(49) and V(2)ENAEK(7) only had 1 or 2 variable amino acid among the reference strains. The 3D model analysis results showed that these epitopes presented as spheres were shown within the context of the complete particle. CONCLUSIONS: In this study, ten McAbs against EMCV VP1 were developed and three B-cells epitopes (2-7aa, 19-25aa and 42-49aa) were defined in VP1. All the results herein will promote the future investigations into the function of VP1 of EMCV and development of diagnostic methods of EMCV. |
format | Online Article Text |
id | pubmed-4297377 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42973772015-01-18 Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine Bai, Juan Chen, Xinhui Jiang, Kangfu Zeshan, Basit Jiang, Ping Virol J Research BACKGROUND: Encephalomyocarditis virus (EMCV) can cause myocarditis, respiratory failure, reproductive failure, and sudden death in pre-weaned piglets, which has been isolated in China. EMCV VP1 protein was one of the most important structural proteins and played an important role in the protective immunity. In this study, 10 monoclonal antibodies (McAbs) against EMCV VP1 were screened and identified. RESULTS: Epitope mapping results indicated that McAbs (6E11, 7A7, 7C9) specifically recognized the linear epitopes V(2)ENAEK(7), McAbs (1D1, 2A2, 5A1, 5A11, 5G1) recognized the epitope F(19)VAQPVY(25), and McAbs 1G8 and 3A9 recognized P(42)IGAFTVK(49). Protein sequence alignment of VP1 with 16 EMCV isolates indicated that the epitope F(19)VAQPVY(25) was conserved in all the reference strains. The epitopes P(42)IGAFTVK(49) and V(2)ENAEK(7) only had 1 or 2 variable amino acid among the reference strains. The 3D model analysis results showed that these epitopes presented as spheres were shown within the context of the complete particle. CONCLUSIONS: In this study, ten McAbs against EMCV VP1 were developed and three B-cells epitopes (2-7aa, 19-25aa and 42-49aa) were defined in VP1. All the results herein will promote the future investigations into the function of VP1 of EMCV and development of diagnostic methods of EMCV. BioMed Central 2014-12-30 /pmc/articles/PMC4297377/ /pubmed/25547933 http://dx.doi.org/10.1186/s12985-014-0226-8 Text en © Bai et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Bai, Juan Chen, Xinhui Jiang, Kangfu Zeshan, Basit Jiang, Ping Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine |
title | Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine |
title_full | Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine |
title_fullStr | Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine |
title_full_unstemmed | Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine |
title_short | Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine |
title_sort | identification of vp1 peptides diagnostic of encephalomyocarditis virus from swine |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4297377/ https://www.ncbi.nlm.nih.gov/pubmed/25547933 http://dx.doi.org/10.1186/s12985-014-0226-8 |
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