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The role of Sox9 in mouse mammary gland development and maintenance of mammary stem and luminal progenitor cells

BACKGROUND: Identification and characterization of molecular controls that regulate mammary stem and progenitor cell homeostasis are critical to our understanding of normal mammary gland development and its pathology. RESULTS: We demonstrate that conditional knockout of Sox9 in the mouse mammary gla...

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Autores principales: Malhotra, Gautam K, Zhao, Xiangshan, Edwards, Emily, Kopp, Janel L, Naramura, Mayumi, Sander, Maike, Band, Hamid, Band, Vimla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4297388/
https://www.ncbi.nlm.nih.gov/pubmed/25527186
http://dx.doi.org/10.1186/s12861-014-0047-4
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author Malhotra, Gautam K
Zhao, Xiangshan
Edwards, Emily
Kopp, Janel L
Naramura, Mayumi
Sander, Maike
Band, Hamid
Band, Vimla
author_facet Malhotra, Gautam K
Zhao, Xiangshan
Edwards, Emily
Kopp, Janel L
Naramura, Mayumi
Sander, Maike
Band, Hamid
Band, Vimla
author_sort Malhotra, Gautam K
collection PubMed
description BACKGROUND: Identification and characterization of molecular controls that regulate mammary stem and progenitor cell homeostasis are critical to our understanding of normal mammary gland development and its pathology. RESULTS: We demonstrate that conditional knockout of Sox9 in the mouse mammary gland results in impaired postnatal development. In short-term lineage tracing in the postnatal mouse mammary gland using Sox9-CreER driven reporters, Sox9 marked primarily the luminal progenitors and bipotent stem/progenitor cells within the basal mammary epithelial compartment. In contrast, long-term lineage tracing studies demonstrate that Sox9+ precursors gave rise to both luminal and myoepithelial cell lineages. Finally, fate mapping of Sox9 deleted cells demonstrates that Sox9 is essential for luminal, but not myoepithelial, lineage commitment and proliferation. CONCLUSIONS: These studies identify Sox9 as a key regulator of mammary gland development and stem/progenitor maintenance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12861-014-0047-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-42973882015-01-18 The role of Sox9 in mouse mammary gland development and maintenance of mammary stem and luminal progenitor cells Malhotra, Gautam K Zhao, Xiangshan Edwards, Emily Kopp, Janel L Naramura, Mayumi Sander, Maike Band, Hamid Band, Vimla BMC Dev Biol Research Article BACKGROUND: Identification and characterization of molecular controls that regulate mammary stem and progenitor cell homeostasis are critical to our understanding of normal mammary gland development and its pathology. RESULTS: We demonstrate that conditional knockout of Sox9 in the mouse mammary gland results in impaired postnatal development. In short-term lineage tracing in the postnatal mouse mammary gland using Sox9-CreER driven reporters, Sox9 marked primarily the luminal progenitors and bipotent stem/progenitor cells within the basal mammary epithelial compartment. In contrast, long-term lineage tracing studies demonstrate that Sox9+ precursors gave rise to both luminal and myoepithelial cell lineages. Finally, fate mapping of Sox9 deleted cells demonstrates that Sox9 is essential for luminal, but not myoepithelial, lineage commitment and proliferation. CONCLUSIONS: These studies identify Sox9 as a key regulator of mammary gland development and stem/progenitor maintenance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12861-014-0047-4) contains supplementary material, which is available to authorized users. BioMed Central 2014-12-20 /pmc/articles/PMC4297388/ /pubmed/25527186 http://dx.doi.org/10.1186/s12861-014-0047-4 Text en © Malhotra et al.; licensee BioMed Central. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Malhotra, Gautam K
Zhao, Xiangshan
Edwards, Emily
Kopp, Janel L
Naramura, Mayumi
Sander, Maike
Band, Hamid
Band, Vimla
The role of Sox9 in mouse mammary gland development and maintenance of mammary stem and luminal progenitor cells
title The role of Sox9 in mouse mammary gland development and maintenance of mammary stem and luminal progenitor cells
title_full The role of Sox9 in mouse mammary gland development and maintenance of mammary stem and luminal progenitor cells
title_fullStr The role of Sox9 in mouse mammary gland development and maintenance of mammary stem and luminal progenitor cells
title_full_unstemmed The role of Sox9 in mouse mammary gland development and maintenance of mammary stem and luminal progenitor cells
title_short The role of Sox9 in mouse mammary gland development and maintenance of mammary stem and luminal progenitor cells
title_sort role of sox9 in mouse mammary gland development and maintenance of mammary stem and luminal progenitor cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4297388/
https://www.ncbi.nlm.nih.gov/pubmed/25527186
http://dx.doi.org/10.1186/s12861-014-0047-4
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