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Enrichment of A Rare Subpopulation of miR-302-Expressing Glioma Cells by Serum Deprivation

OBJECTIVE: MiR-302-367 is a cluster of polycistronic microRNAs that are exclusively expressed in embryonic stem (ES) cells. The miR-302-367 promoter is functional during embryonic development but is turned off in later stages. Motivated by the cancer stem cell hypothesis, we explored the potential e...

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Autores principales: Rafiee, Mahmoud-Reza, Malekzadeh Shafaroudi, Afsaneh, Rohban, Sara, Khayatzadeh, Hamid, Kalhor, Hamid Reza, Mowla, Seyed Javad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4297488/
https://www.ncbi.nlm.nih.gov/pubmed/25685740
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author Rafiee, Mahmoud-Reza
Malekzadeh Shafaroudi, Afsaneh
Rohban, Sara
Khayatzadeh, Hamid
Kalhor, Hamid Reza
Mowla, Seyed Javad
author_facet Rafiee, Mahmoud-Reza
Malekzadeh Shafaroudi, Afsaneh
Rohban, Sara
Khayatzadeh, Hamid
Kalhor, Hamid Reza
Mowla, Seyed Javad
author_sort Rafiee, Mahmoud-Reza
collection PubMed
description OBJECTIVE: MiR-302-367 is a cluster of polycistronic microRNAs that are exclusively expressed in embryonic stem (ES) cells. The miR-302-367 promoter is functional during embryonic development but is turned off in later stages. Motivated by the cancer stem cell hypothesis, we explored the potential expression of miR-302 in brain tumor cell lines. MATERIALS AND METHODS: In the present experimental study, we have tried to expand our knowledge on the expression pattern and functionality of miR302 cluster by quantifying its expression in a series of glioma (A-172, 1321N1, U87MG) and medulloblastoma (DAOY) cell lines. To further assess the functionality of miR-302 in these cell lines, we cloned its promoter core region upstream of the enhanced green fluorescent protein (EGFP) or luciferase encoding genes. RESULTS: Our data demonstrated a very low expression of miR-302 in glioma cell lines, compared with that of embryonal carcinoma cell line NT2 being used as a positive control. The expression of miR-302 promoter-EGFP construct in the aforementioned cell lines demonstrated GFP expression in a rare subpopulation of the cells. Serum deprivation led to the generation of tumorospheres, enrichment of miR-302 positive cells and upregulation of a number of pluripotency genes. CONCLUSION: Taken together, our data suggest that miR-302 could potentially be used as a novel putative cancer stem cell marker to identify and target cancer stem cells within tumor tissues.
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spelling pubmed-42974882015-02-13 Enrichment of A Rare Subpopulation of miR-302-Expressing Glioma Cells by Serum Deprivation Rafiee, Mahmoud-Reza Malekzadeh Shafaroudi, Afsaneh Rohban, Sara Khayatzadeh, Hamid Kalhor, Hamid Reza Mowla, Seyed Javad Cell J Original Article OBJECTIVE: MiR-302-367 is a cluster of polycistronic microRNAs that are exclusively expressed in embryonic stem (ES) cells. The miR-302-367 promoter is functional during embryonic development but is turned off in later stages. Motivated by the cancer stem cell hypothesis, we explored the potential expression of miR-302 in brain tumor cell lines. MATERIALS AND METHODS: In the present experimental study, we have tried to expand our knowledge on the expression pattern and functionality of miR302 cluster by quantifying its expression in a series of glioma (A-172, 1321N1, U87MG) and medulloblastoma (DAOY) cell lines. To further assess the functionality of miR-302 in these cell lines, we cloned its promoter core region upstream of the enhanced green fluorescent protein (EGFP) or luciferase encoding genes. RESULTS: Our data demonstrated a very low expression of miR-302 in glioma cell lines, compared with that of embryonal carcinoma cell line NT2 being used as a positive control. The expression of miR-302 promoter-EGFP construct in the aforementioned cell lines demonstrated GFP expression in a rare subpopulation of the cells. Serum deprivation led to the generation of tumorospheres, enrichment of miR-302 positive cells and upregulation of a number of pluripotency genes. CONCLUSION: Taken together, our data suggest that miR-302 could potentially be used as a novel putative cancer stem cell marker to identify and target cancer stem cells within tumor tissues. Royan Institute 2015 2015-01-13 /pmc/articles/PMC4297488/ /pubmed/25685740 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Rafiee, Mahmoud-Reza
Malekzadeh Shafaroudi, Afsaneh
Rohban, Sara
Khayatzadeh, Hamid
Kalhor, Hamid Reza
Mowla, Seyed Javad
Enrichment of A Rare Subpopulation of miR-302-Expressing Glioma Cells by Serum Deprivation
title Enrichment of A Rare Subpopulation of miR-302-Expressing Glioma Cells by Serum Deprivation
title_full Enrichment of A Rare Subpopulation of miR-302-Expressing Glioma Cells by Serum Deprivation
title_fullStr Enrichment of A Rare Subpopulation of miR-302-Expressing Glioma Cells by Serum Deprivation
title_full_unstemmed Enrichment of A Rare Subpopulation of miR-302-Expressing Glioma Cells by Serum Deprivation
title_short Enrichment of A Rare Subpopulation of miR-302-Expressing Glioma Cells by Serum Deprivation
title_sort enrichment of a rare subpopulation of mir-302-expressing glioma cells by serum deprivation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4297488/
https://www.ncbi.nlm.nih.gov/pubmed/25685740
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