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Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1)

• Premise of the study: Microsatellite markers were developed to investigate population genetic structure in the threatened species Arnica montana. • Methods and Results: Fourteen microsatellite markers with di-, tetra-, and hexanucleotide repeat motifs were developed for A. montana using 454 pyrose...

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Autores principales: Duwe, Virginia K., Ismail, Sascha A., Buser, Andres, Sossai, Esther, Borsch, Thomas, Muller, Ludo A. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4298232/
https://www.ncbi.nlm.nih.gov/pubmed/25606354
http://dx.doi.org/10.3732/apps.1400091
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author Duwe, Virginia K.
Ismail, Sascha A.
Buser, Andres
Sossai, Esther
Borsch, Thomas
Muller, Ludo A. H.
author_facet Duwe, Virginia K.
Ismail, Sascha A.
Buser, Andres
Sossai, Esther
Borsch, Thomas
Muller, Ludo A. H.
author_sort Duwe, Virginia K.
collection PubMed
description • Premise of the study: Microsatellite markers were developed to investigate population genetic structure in the threatened species Arnica montana. • Methods and Results: Fourteen microsatellite markers with di-, tetra-, and hexanucleotide repeat motifs were developed for A. montana using 454 pyrosequencing without and with library-enrichment methods, resulting in 56,545 sequence reads and 14,467 sequence reads, respectively. All loci showed a high level of polymorphism, with allele numbers ranging from four to 11 in five individuals from five populations (25 samples) and an expected heterozygosity ranging from 0.192 to 0.648 across the loci. • Conclusions: This set of microsatellite markers is the first one described for A. montana and will facilitate conservation genetic applications as well as the understanding of phylogeographic patterns in this species.
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spelling pubmed-42982322015-01-20 Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1) Duwe, Virginia K. Ismail, Sascha A. Buser, Andres Sossai, Esther Borsch, Thomas Muller, Ludo A. H. Appl Plant Sci Primer Note • Premise of the study: Microsatellite markers were developed to investigate population genetic structure in the threatened species Arnica montana. • Methods and Results: Fourteen microsatellite markers with di-, tetra-, and hexanucleotide repeat motifs were developed for A. montana using 454 pyrosequencing without and with library-enrichment methods, resulting in 56,545 sequence reads and 14,467 sequence reads, respectively. All loci showed a high level of polymorphism, with allele numbers ranging from four to 11 in five individuals from five populations (25 samples) and an expected heterozygosity ranging from 0.192 to 0.648 across the loci. • Conclusions: This set of microsatellite markers is the first one described for A. montana and will facilitate conservation genetic applications as well as the understanding of phylogeographic patterns in this species. Botanical Society of America 2015-01-05 /pmc/articles/PMC4298232/ /pubmed/25606354 http://dx.doi.org/10.3732/apps.1400091 Text en © 2015 Duwe et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Duwe, Virginia K.
Ismail, Sascha A.
Buser, Andres
Sossai, Esther
Borsch, Thomas
Muller, Ludo A. H.
Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1)
title Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1)
title_full Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1)
title_fullStr Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1)
title_full_unstemmed Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1)
title_short Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1)
title_sort fourteen polymorphic microsatellite markers for the threatened arnica montana (asteraceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4298232/
https://www.ncbi.nlm.nih.gov/pubmed/25606354
http://dx.doi.org/10.3732/apps.1400091
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