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Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1)
• Premise of the study: Microsatellite markers were developed to investigate population genetic structure in the threatened species Arnica montana. • Methods and Results: Fourteen microsatellite markers with di-, tetra-, and hexanucleotide repeat motifs were developed for A. montana using 454 pyrose...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4298232/ https://www.ncbi.nlm.nih.gov/pubmed/25606354 http://dx.doi.org/10.3732/apps.1400091 |
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author | Duwe, Virginia K. Ismail, Sascha A. Buser, Andres Sossai, Esther Borsch, Thomas Muller, Ludo A. H. |
author_facet | Duwe, Virginia K. Ismail, Sascha A. Buser, Andres Sossai, Esther Borsch, Thomas Muller, Ludo A. H. |
author_sort | Duwe, Virginia K. |
collection | PubMed |
description | • Premise of the study: Microsatellite markers were developed to investigate population genetic structure in the threatened species Arnica montana. • Methods and Results: Fourteen microsatellite markers with di-, tetra-, and hexanucleotide repeat motifs were developed for A. montana using 454 pyrosequencing without and with library-enrichment methods, resulting in 56,545 sequence reads and 14,467 sequence reads, respectively. All loci showed a high level of polymorphism, with allele numbers ranging from four to 11 in five individuals from five populations (25 samples) and an expected heterozygosity ranging from 0.192 to 0.648 across the loci. • Conclusions: This set of microsatellite markers is the first one described for A. montana and will facilitate conservation genetic applications as well as the understanding of phylogeographic patterns in this species. |
format | Online Article Text |
id | pubmed-4298232 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-42982322015-01-20 Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1) Duwe, Virginia K. Ismail, Sascha A. Buser, Andres Sossai, Esther Borsch, Thomas Muller, Ludo A. H. Appl Plant Sci Primer Note • Premise of the study: Microsatellite markers were developed to investigate population genetic structure in the threatened species Arnica montana. • Methods and Results: Fourteen microsatellite markers with di-, tetra-, and hexanucleotide repeat motifs were developed for A. montana using 454 pyrosequencing without and with library-enrichment methods, resulting in 56,545 sequence reads and 14,467 sequence reads, respectively. All loci showed a high level of polymorphism, with allele numbers ranging from four to 11 in five individuals from five populations (25 samples) and an expected heterozygosity ranging from 0.192 to 0.648 across the loci. • Conclusions: This set of microsatellite markers is the first one described for A. montana and will facilitate conservation genetic applications as well as the understanding of phylogeographic patterns in this species. Botanical Society of America 2015-01-05 /pmc/articles/PMC4298232/ /pubmed/25606354 http://dx.doi.org/10.3732/apps.1400091 Text en © 2015 Duwe et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note Duwe, Virginia K. Ismail, Sascha A. Buser, Andres Sossai, Esther Borsch, Thomas Muller, Ludo A. H. Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1) |
title | Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1) |
title_full | Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1) |
title_fullStr | Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1) |
title_full_unstemmed | Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1) |
title_short | Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae)(1) |
title_sort | fourteen polymorphic microsatellite markers for the threatened arnica montana (asteraceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4298232/ https://www.ncbi.nlm.nih.gov/pubmed/25606354 http://dx.doi.org/10.3732/apps.1400091 |
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