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Dinuclear Ruthenium(II) Complexes as Two-Photon, Time-Resolved Emission Microscopy Probes for Cellular DNA**
The first transition-metal complex-based two-photon absorbing luminescence lifetime probes for cellular DNA are presented. This allows cell imaging of DNA free from endogenous fluorophores and potentially facilitates deep tissue imaging. In this initial study, ruthenium(II) luminophores are used as...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
WILEY-VCH Verlag
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4298790/ https://www.ncbi.nlm.nih.gov/pubmed/24458590 http://dx.doi.org/10.1002/anie.201309427 |
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author | Baggaley, Elizabeth Gill, Martin R Green, Nicola H Turton, David Sazanovich, Igor V Botchway, Stanley W Smythe, Carl Haycock, John W Weinstein, Julia A Thomas, Jim A |
author_facet | Baggaley, Elizabeth Gill, Martin R Green, Nicola H Turton, David Sazanovich, Igor V Botchway, Stanley W Smythe, Carl Haycock, John W Weinstein, Julia A Thomas, Jim A |
author_sort | Baggaley, Elizabeth |
collection | PubMed |
description | The first transition-metal complex-based two-photon absorbing luminescence lifetime probes for cellular DNA are presented. This allows cell imaging of DNA free from endogenous fluorophores and potentially facilitates deep tissue imaging. In this initial study, ruthenium(II) luminophores are used as phosphorescent lifetime imaging microscopy (PLIM) probes for nuclear DNA in both live and fixed cells. The DNA-bound probes display characteristic emission lifetimes of more than 160 ns, while shorter-lived cytoplasmic emission is also observed. These timescales are orders of magnitude longer than conventional FLIM, leading to previously unattainable levels of sensitivity, and autofluorescence-free imaging. |
format | Online Article Text |
id | pubmed-4298790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | WILEY-VCH Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-42987902015-01-27 Dinuclear Ruthenium(II) Complexes as Two-Photon, Time-Resolved Emission Microscopy Probes for Cellular DNA** Baggaley, Elizabeth Gill, Martin R Green, Nicola H Turton, David Sazanovich, Igor V Botchway, Stanley W Smythe, Carl Haycock, John W Weinstein, Julia A Thomas, Jim A Angew Chem Int Ed Engl Communications The first transition-metal complex-based two-photon absorbing luminescence lifetime probes for cellular DNA are presented. This allows cell imaging of DNA free from endogenous fluorophores and potentially facilitates deep tissue imaging. In this initial study, ruthenium(II) luminophores are used as phosphorescent lifetime imaging microscopy (PLIM) probes for nuclear DNA in both live and fixed cells. The DNA-bound probes display characteristic emission lifetimes of more than 160 ns, while shorter-lived cytoplasmic emission is also observed. These timescales are orders of magnitude longer than conventional FLIM, leading to previously unattainable levels of sensitivity, and autofluorescence-free imaging. WILEY-VCH Verlag 2014-03-24 2014-01-23 /pmc/articles/PMC4298790/ /pubmed/24458590 http://dx.doi.org/10.1002/anie.201309427 Text en © 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited https://creativecommons.org/licenses/by/4.0/ © 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited |
spellingShingle | Communications Baggaley, Elizabeth Gill, Martin R Green, Nicola H Turton, David Sazanovich, Igor V Botchway, Stanley W Smythe, Carl Haycock, John W Weinstein, Julia A Thomas, Jim A Dinuclear Ruthenium(II) Complexes as Two-Photon, Time-Resolved Emission Microscopy Probes for Cellular DNA** |
title | Dinuclear Ruthenium(II) Complexes as Two-Photon, Time-Resolved Emission Microscopy Probes for Cellular DNA** |
title_full | Dinuclear Ruthenium(II) Complexes as Two-Photon, Time-Resolved Emission Microscopy Probes for Cellular DNA** |
title_fullStr | Dinuclear Ruthenium(II) Complexes as Two-Photon, Time-Resolved Emission Microscopy Probes for Cellular DNA** |
title_full_unstemmed | Dinuclear Ruthenium(II) Complexes as Two-Photon, Time-Resolved Emission Microscopy Probes for Cellular DNA** |
title_short | Dinuclear Ruthenium(II) Complexes as Two-Photon, Time-Resolved Emission Microscopy Probes for Cellular DNA** |
title_sort | dinuclear ruthenium(ii) complexes as two-photon, time-resolved emission microscopy probes for cellular dna** |
topic | Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4298790/ https://www.ncbi.nlm.nih.gov/pubmed/24458590 http://dx.doi.org/10.1002/anie.201309427 |
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