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Effects of Exendin-4 on Male Reproductive Parameters of D-Galactose Induced Aging Mouse Model
PURPOSE: The purpose of this study was to evaluate the role of exendin-4 on reproductive alteration in a D-galactose-induced aging mouse model. MATERIALS AND METHODS: In this experimental study, 72 male Naval Medical Research Institute mice (20~25 g) were randomly divided into six groups: control, e...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society for Sexual Medicine and Andrology
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4298821/ https://www.ncbi.nlm.nih.gov/pubmed/25606567 http://dx.doi.org/10.5534/wjmh.2014.32.3.176 |
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author | Ahangarpour, Akram Oroojan, Ali Akbar Heidari, Hamid |
author_facet | Ahangarpour, Akram Oroojan, Ali Akbar Heidari, Hamid |
author_sort | Ahangarpour, Akram |
collection | PubMed |
description | PURPOSE: The purpose of this study was to evaluate the role of exendin-4 on reproductive alteration in a D-galactose-induced aging mouse model. MATERIALS AND METHODS: In this experimental study, 72 male Naval Medical Research Institute mice (20~25 g) were randomly divided into six groups: control, exendin-4 (1 nmol/kg), exendin-4 (10 nmol/kg), D-galactose (500 mg/kg), D-galactose+exendin-4 (1 nmol/kg), and D-galactose+exendin-4 (10 nmol/kg). The aging model animals were gavaged with D-galactose for six weeks, and exendin-4 was injected intraperitoneally in the last 10 days. At the end of treatment serum luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone levels were evaluated and the cauda epididymis and testis were removed to analyze the sperm count and testis morphology. RESULTS: The testis weight and volume decreased in the D-galactose group (p<0.01 and p<0.05) respectively. Exendin-4 (1, 10 nmol/kg) increased these parameters in the normal and aging mouse models. Serum LH and FSH levels increased and the sperm count decreased in the D-galactose group (p<0.05). Further, exendin-4 (1 nmol/kg) decreased LH and FSH levels and increased the serum testosterone level and sperm count in both normal and aging animals. CONCLUSIONS: D-galactose can induce aging alternations in the male reproductive system such as decreased sperm count and increased serum LH and FSH levels through reactive oxygen species over production and reduced antioxidant enzyme activity. Further, co-administration of exendin-4 reduced reproductive complications of D-galactose in an aging mouse model. |
format | Online Article Text |
id | pubmed-4298821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Korean Society for Sexual Medicine and Andrology |
record_format | MEDLINE/PubMed |
spelling | pubmed-42988212015-01-20 Effects of Exendin-4 on Male Reproductive Parameters of D-Galactose Induced Aging Mouse Model Ahangarpour, Akram Oroojan, Ali Akbar Heidari, Hamid World J Mens Health Original Article PURPOSE: The purpose of this study was to evaluate the role of exendin-4 on reproductive alteration in a D-galactose-induced aging mouse model. MATERIALS AND METHODS: In this experimental study, 72 male Naval Medical Research Institute mice (20~25 g) were randomly divided into six groups: control, exendin-4 (1 nmol/kg), exendin-4 (10 nmol/kg), D-galactose (500 mg/kg), D-galactose+exendin-4 (1 nmol/kg), and D-galactose+exendin-4 (10 nmol/kg). The aging model animals were gavaged with D-galactose for six weeks, and exendin-4 was injected intraperitoneally in the last 10 days. At the end of treatment serum luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone levels were evaluated and the cauda epididymis and testis were removed to analyze the sperm count and testis morphology. RESULTS: The testis weight and volume decreased in the D-galactose group (p<0.01 and p<0.05) respectively. Exendin-4 (1, 10 nmol/kg) increased these parameters in the normal and aging mouse models. Serum LH and FSH levels increased and the sperm count decreased in the D-galactose group (p<0.05). Further, exendin-4 (1 nmol/kg) decreased LH and FSH levels and increased the serum testosterone level and sperm count in both normal and aging animals. CONCLUSIONS: D-galactose can induce aging alternations in the male reproductive system such as decreased sperm count and increased serum LH and FSH levels through reactive oxygen species over production and reduced antioxidant enzyme activity. Further, co-administration of exendin-4 reduced reproductive complications of D-galactose in an aging mouse model. Korean Society for Sexual Medicine and Andrology 2014-12 2014-12-29 /pmc/articles/PMC4298821/ /pubmed/25606567 http://dx.doi.org/10.5534/wjmh.2014.32.3.176 Text en Copyright © 2014 Korean Society for Sexual Medicine and Andrology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ahangarpour, Akram Oroojan, Ali Akbar Heidari, Hamid Effects of Exendin-4 on Male Reproductive Parameters of D-Galactose Induced Aging Mouse Model |
title | Effects of Exendin-4 on Male Reproductive Parameters of D-Galactose Induced Aging Mouse Model |
title_full | Effects of Exendin-4 on Male Reproductive Parameters of D-Galactose Induced Aging Mouse Model |
title_fullStr | Effects of Exendin-4 on Male Reproductive Parameters of D-Galactose Induced Aging Mouse Model |
title_full_unstemmed | Effects of Exendin-4 on Male Reproductive Parameters of D-Galactose Induced Aging Mouse Model |
title_short | Effects of Exendin-4 on Male Reproductive Parameters of D-Galactose Induced Aging Mouse Model |
title_sort | effects of exendin-4 on male reproductive parameters of d-galactose induced aging mouse model |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4298821/ https://www.ncbi.nlm.nih.gov/pubmed/25606567 http://dx.doi.org/10.5534/wjmh.2014.32.3.176 |
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