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Genetic variability of microRNA regulome in human

MicroRNAs are currently being extensively studied due to their important role as post-transcriptional regulators. During miRNA biogenesis, precursors undergo two cleavage steps performed by Drosha-DGCR8 (Microprocessor) cleaving of pri-miRNA to produce pre-miRNA and Dicer-mediated cleaving to create...

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Autores principales: Obsteter, Jana, Dovc, Peter, Kunej, Tanja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4299713/
https://www.ncbi.nlm.nih.gov/pubmed/25629077
http://dx.doi.org/10.1002/mgg3.110
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author Obsteter, Jana
Dovc, Peter
Kunej, Tanja
author_facet Obsteter, Jana
Dovc, Peter
Kunej, Tanja
author_sort Obsteter, Jana
collection PubMed
description MicroRNAs are currently being extensively studied due to their important role as post-transcriptional regulators. During miRNA biogenesis, precursors undergo two cleavage steps performed by Drosha-DGCR8 (Microprocessor) cleaving of pri-miRNA to produce pre-miRNA and Dicer-mediated cleaving to create mature miRNA. Genetic variants within human miRNA regulome have been shown to influence miRNA expression, target interaction and to affect the phenotype. In this study, we reviewed the literature, existing bioinformatics tools and catalogs associated with polymorphic miRNA regulome, and organized them into four categories: (1) polymorphisms located within miRNA genes (miR-SNPs), (2) transcription factor-binding sites/miRNA regulatory regions (miR-rSNPs), (3) miRNA target sites (miR-TS-SNPs), and 4. miRNA silencing machinery (miR-SM-SNPs). Since the miR-SM-SNPs have not been systematically studied yet, we have collected polymorphisms associated with miRNA silencing machinery. We have developed two catalogs containing genetic variability within: (1) genes encoding three main catalytic components of the silencing machinery, DROSHA, DGCR8, and DICER1; (2) miRNA genes itself, overlapping Drosha and Dicer cleavage sites. The developed resource of polymorphisms is available online (http://www.integratomics-time.com/miRNA-regulome) and will be useful for further functional studies and development of biomarkers associated with diseases and phenotypic traits.
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spelling pubmed-42997132015-01-27 Genetic variability of microRNA regulome in human Obsteter, Jana Dovc, Peter Kunej, Tanja Mol Genet Genomic Med Original Articles MicroRNAs are currently being extensively studied due to their important role as post-transcriptional regulators. During miRNA biogenesis, precursors undergo two cleavage steps performed by Drosha-DGCR8 (Microprocessor) cleaving of pri-miRNA to produce pre-miRNA and Dicer-mediated cleaving to create mature miRNA. Genetic variants within human miRNA regulome have been shown to influence miRNA expression, target interaction and to affect the phenotype. In this study, we reviewed the literature, existing bioinformatics tools and catalogs associated with polymorphic miRNA regulome, and organized them into four categories: (1) polymorphisms located within miRNA genes (miR-SNPs), (2) transcription factor-binding sites/miRNA regulatory regions (miR-rSNPs), (3) miRNA target sites (miR-TS-SNPs), and 4. miRNA silencing machinery (miR-SM-SNPs). Since the miR-SM-SNPs have not been systematically studied yet, we have collected polymorphisms associated with miRNA silencing machinery. We have developed two catalogs containing genetic variability within: (1) genes encoding three main catalytic components of the silencing machinery, DROSHA, DGCR8, and DICER1; (2) miRNA genes itself, overlapping Drosha and Dicer cleavage sites. The developed resource of polymorphisms is available online (http://www.integratomics-time.com/miRNA-regulome) and will be useful for further functional studies and development of biomarkers associated with diseases and phenotypic traits. BlackWell Publishing Ltd 2015-01 2014-09-15 /pmc/articles/PMC4299713/ /pubmed/25629077 http://dx.doi.org/10.1002/mgg3.110 Text en © 2014 The Authors. Molecular Genetics & Genomic Medicine published by Wiley Periodicals, Inc. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Obsteter, Jana
Dovc, Peter
Kunej, Tanja
Genetic variability of microRNA regulome in human
title Genetic variability of microRNA regulome in human
title_full Genetic variability of microRNA regulome in human
title_fullStr Genetic variability of microRNA regulome in human
title_full_unstemmed Genetic variability of microRNA regulome in human
title_short Genetic variability of microRNA regulome in human
title_sort genetic variability of microrna regulome in human
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4299713/
https://www.ncbi.nlm.nih.gov/pubmed/25629077
http://dx.doi.org/10.1002/mgg3.110
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