Stimulation of human damaged sperm motility with hydrogen molecule

BACKGROUND: Sperm motility is a critical factor in male fertility. Low motility can be caused by a variety factors including abnormal spermatogenesis, oxidative damage, or depletion of intracellular ATP. Recent findings indicate that hydrogen molecule (H(2)) selectively reduces toxic reactive oxygen species. In this study, we investigated the effects of H(2) on human sperm motility in vitro. METHODS: Experimentally damaged sperm suspensions from patients left at room temperature for > 5 days or frozen immediately after ejaculation were used. After exposure with H(2), their forward motility was measured with a counting chamber. A time-lapse movie was recorded to analyze sperm swimming speed. Mitochondria were stained with a membrane potential-sensitive dye. RESULTS: H(2) treatment significantly improved the rate of forward motility, whereas treatment with nitrogen gas did not. While treatment for 30 min was sufficient to improve motility, it did not affect sperm swimming speed. After 24 h, retreatment with H(2) increased the motility again. H(2) treatment also increased mitochondrial membrane potential. Forward motility of low motile frozen-thawed sperm from patients significantly improved with cleavage medium containing H(2). CONCLUSIONS: Our results illustrated that H(2) treatment stimulates low sperm motility. H(2) is a new promising tool for male infertility treatments.