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Stimulation of human damaged sperm motility with hydrogen molecule

BACKGROUND: Sperm motility is a critical factor in male fertility. Low motility can be caused by a variety factors including abnormal spermatogenesis, oxidative damage, or depletion of intracellular ATP. Recent findings indicate that hydrogen molecule (H(2)) selectively reduces toxic reactive oxygen...

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Autores principales: Nakata, Kumiko, Yamashita, Naoki, Noda, Yoshihiro, Ohsawa, Ikuroh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300028/
https://www.ncbi.nlm.nih.gov/pubmed/25649433
http://dx.doi.org/10.1186/s13618-014-0023-x
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author Nakata, Kumiko
Yamashita, Naoki
Noda, Yoshihiro
Ohsawa, Ikuroh
author_facet Nakata, Kumiko
Yamashita, Naoki
Noda, Yoshihiro
Ohsawa, Ikuroh
author_sort Nakata, Kumiko
collection PubMed
description BACKGROUND: Sperm motility is a critical factor in male fertility. Low motility can be caused by a variety factors including abnormal spermatogenesis, oxidative damage, or depletion of intracellular ATP. Recent findings indicate that hydrogen molecule (H(2)) selectively reduces toxic reactive oxygen species. In this study, we investigated the effects of H(2) on human sperm motility in vitro. METHODS: Experimentally damaged sperm suspensions from patients left at room temperature for > 5 days or frozen immediately after ejaculation were used. After exposure with H(2), their forward motility was measured with a counting chamber. A time-lapse movie was recorded to analyze sperm swimming speed. Mitochondria were stained with a membrane potential-sensitive dye. RESULTS: H(2) treatment significantly improved the rate of forward motility, whereas treatment with nitrogen gas did not. While treatment for 30 min was sufficient to improve motility, it did not affect sperm swimming speed. After 24 h, retreatment with H(2) increased the motility again. H(2) treatment also increased mitochondrial membrane potential. Forward motility of low motile frozen-thawed sperm from patients significantly improved with cleavage medium containing H(2). CONCLUSIONS: Our results illustrated that H(2) treatment stimulates low sperm motility. H(2) is a new promising tool for male infertility treatments.
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spelling pubmed-43000282015-02-03 Stimulation of human damaged sperm motility with hydrogen molecule Nakata, Kumiko Yamashita, Naoki Noda, Yoshihiro Ohsawa, Ikuroh Med Gas Res Research BACKGROUND: Sperm motility is a critical factor in male fertility. Low motility can be caused by a variety factors including abnormal spermatogenesis, oxidative damage, or depletion of intracellular ATP. Recent findings indicate that hydrogen molecule (H(2)) selectively reduces toxic reactive oxygen species. In this study, we investigated the effects of H(2) on human sperm motility in vitro. METHODS: Experimentally damaged sperm suspensions from patients left at room temperature for > 5 days or frozen immediately after ejaculation were used. After exposure with H(2), their forward motility was measured with a counting chamber. A time-lapse movie was recorded to analyze sperm swimming speed. Mitochondria were stained with a membrane potential-sensitive dye. RESULTS: H(2) treatment significantly improved the rate of forward motility, whereas treatment with nitrogen gas did not. While treatment for 30 min was sufficient to improve motility, it did not affect sperm swimming speed. After 24 h, retreatment with H(2) increased the motility again. H(2) treatment also increased mitochondrial membrane potential. Forward motility of low motile frozen-thawed sperm from patients significantly improved with cleavage medium containing H(2). CONCLUSIONS: Our results illustrated that H(2) treatment stimulates low sperm motility. H(2) is a new promising tool for male infertility treatments. BioMed Central 2015-01-10 /pmc/articles/PMC4300028/ /pubmed/25649433 http://dx.doi.org/10.1186/s13618-014-0023-x Text en © Nakata et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Nakata, Kumiko
Yamashita, Naoki
Noda, Yoshihiro
Ohsawa, Ikuroh
Stimulation of human damaged sperm motility with hydrogen molecule
title Stimulation of human damaged sperm motility with hydrogen molecule
title_full Stimulation of human damaged sperm motility with hydrogen molecule
title_fullStr Stimulation of human damaged sperm motility with hydrogen molecule
title_full_unstemmed Stimulation of human damaged sperm motility with hydrogen molecule
title_short Stimulation of human damaged sperm motility with hydrogen molecule
title_sort stimulation of human damaged sperm motility with hydrogen molecule
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300028/
https://www.ncbi.nlm.nih.gov/pubmed/25649433
http://dx.doi.org/10.1186/s13618-014-0023-x
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