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Genome engineering for improved recombinant protein expression in Escherichia coli
A metabolic engineering perspective which views recombinant protein expression as a multistep pathway allows us to move beyond vector design and identify the downstream rate limiting steps in expression. In E.coli these are typically at the translational level and the supply of precursors in the for...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300154/ https://www.ncbi.nlm.nih.gov/pubmed/25523647 http://dx.doi.org/10.1186/s12934-014-0177-1 |