A Real-Time PCR for Detection and Quantification of Mycoplasma ovipneumoniae

A real-time PCR for detection and quantification of M. ovipneumoniae was developed using 9 recently sequenced M. ovipneumoniae genomes and primers targeting a putative adhesin gene p113. The assay proved to be specific and sensitive (with a detection limit of 22 genomic DNA) and could quantify M. ov...

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Autores principales: YANG, Falong, DAO, Xiaofang, RODRIGUEZ-PALACIOS, Alex, FENG, Xufei, TANG, Cheng, YANG, Xiaonong, YUE, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2014
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300380/
https://www.ncbi.nlm.nih.gov/pubmed/25649947
http://dx.doi.org/10.1292/jvms.14-0094
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author YANG, Falong
DAO, Xiaofang
RODRIGUEZ-PALACIOS, Alex
FENG, Xufei
TANG, Cheng
YANG, Xiaonong
YUE, Hua
author_facet YANG, Falong
DAO, Xiaofang
RODRIGUEZ-PALACIOS, Alex
FENG, Xufei
TANG, Cheng
YANG, Xiaonong
YUE, Hua
author_sort YANG, Falong
collection PubMed
description A real-time PCR for detection and quantification of M. ovipneumoniae was developed using 9 recently sequenced M. ovipneumoniae genomes and primers targeting a putative adhesin gene p113. The assay proved to be specific and sensitive (with a detection limit of 22 genomic DNA) and could quantify M. ovipneumoniae DNA over a wide linear range, from 2.2 × 10(2) to 2.2 × 10(7) genomes.
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spelling pubmed-43003802015-02-06 A Real-Time PCR for Detection and Quantification of Mycoplasma ovipneumoniae YANG, Falong DAO, Xiaofang RODRIGUEZ-PALACIOS, Alex FENG, Xufei TANG, Cheng YANG, Xiaonong YUE, Hua J Vet Med Sci Bacteriology A real-time PCR for detection and quantification of M. ovipneumoniae was developed using 9 recently sequenced M. ovipneumoniae genomes and primers targeting a putative adhesin gene p113. The assay proved to be specific and sensitive (with a detection limit of 22 genomic DNA) and could quantify M. ovipneumoniae DNA over a wide linear range, from 2.2 × 10(2) to 2.2 × 10(7) genomes. The Japanese Society of Veterinary Science 2014-08-26 2014-12 /pmc/articles/PMC4300380/ /pubmed/25649947 http://dx.doi.org/10.1292/jvms.14-0094 Text en ©2014 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Bacteriology
YANG, Falong
DAO, Xiaofang
RODRIGUEZ-PALACIOS, Alex
FENG, Xufei
TANG, Cheng
YANG, Xiaonong
YUE, Hua
A Real-Time PCR for Detection and Quantification of Mycoplasma ovipneumoniae
title A Real-Time PCR for Detection and Quantification of Mycoplasma ovipneumoniae
title_full A Real-Time PCR for Detection and Quantification of Mycoplasma ovipneumoniae
title_fullStr A Real-Time PCR for Detection and Quantification of Mycoplasma ovipneumoniae
title_full_unstemmed A Real-Time PCR for Detection and Quantification of Mycoplasma ovipneumoniae
title_short A Real-Time PCR for Detection and Quantification of Mycoplasma ovipneumoniae
title_sort real-time pcr for detection and quantification of mycoplasma ovipneumoniae
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300380/
https://www.ncbi.nlm.nih.gov/pubmed/25649947
http://dx.doi.org/10.1292/jvms.14-0094
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