Molecular Cloning and Gene Expression of Canine Apoptosis Inhibitor of Macrophage

Apoptosis inhibitor of macrophage (AIM) plays roles in survival of macrophages. In this study, we cloned canine AIM cDNA and observed its transcriptional expression levels in various tissues. The coding sequence of canine AIM was 1,023 bp encoding 340 amino acid residues, which had around 65% homolo...

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Detalles Bibliográficos
Autores principales: TOMURA, Shintaro, UCHIDA, Mona, YONEZAWA, Tomohiro, KOBAYASHI, Masato, BONKOBARA, Makoto, ARAI, Satoko, MIYAZAKI, Toru, TAMAHARA, Satoshi, MATSUKI, Naoaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2014
Materias:
Internal Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300382/
https://www.ncbi.nlm.nih.gov/pubmed/25649949
http://dx.doi.org/10.1292/jvms.14-0166
Descripción
Sumario:Apoptosis inhibitor of macrophage (AIM) plays roles in survival of macrophages. In this study, we cloned canine AIM cDNA and observed its transcriptional expression levels in various tissues. The coding sequence of canine AIM was 1,023 bp encoding 340 amino acid residues, which had around 65% homology with those of the human, mouse and rat. Transcriptional expression of AIM was observed in the spleen, lung, liver and lymph node, which confirmed the expression of canine AIM in tissue macrophages. Moreover, AIM was highly expressed in one of the canine histiocytic sarcoma cell lines. CD36, the receptor of AIM, was also expressed in various tissues and these cell lines. These findings are useful to reveal the actual functions of canine AIM.

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