Molecular Cloning and Gene Expression of Canine Apoptosis Inhibitor of Macrophage

Apoptosis inhibitor of macrophage (AIM) plays roles in survival of macrophages. In this study, we cloned canine AIM cDNA and observed its transcriptional expression levels in various tissues. The coding sequence of canine AIM was 1,023 bp encoding 340 amino acid residues, which had around 65% homolo...

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Autores principales: TOMURA, Shintaro, UCHIDA, Mona, YONEZAWA, Tomohiro, KOBAYASHI, Masato, BONKOBARA, Makoto, ARAI, Satoko, MIYAZAKI, Toru, TAMAHARA, Satoshi, MATSUKI, Naoaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2014
Materias:
Internal Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300382/
https://www.ncbi.nlm.nih.gov/pubmed/25649949
http://dx.doi.org/10.1292/jvms.14-0166
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author TOMURA, Shintaro
UCHIDA, Mona
YONEZAWA, Tomohiro
KOBAYASHI, Masato
BONKOBARA, Makoto
ARAI, Satoko
MIYAZAKI, Toru
TAMAHARA, Satoshi
MATSUKI, Naoaki
author_facet TOMURA, Shintaro
UCHIDA, Mona
YONEZAWA, Tomohiro
KOBAYASHI, Masato
BONKOBARA, Makoto
ARAI, Satoko
MIYAZAKI, Toru
TAMAHARA, Satoshi
MATSUKI, Naoaki
author_sort TOMURA, Shintaro
collection PubMed
description Apoptosis inhibitor of macrophage (AIM) plays roles in survival of macrophages. In this study, we cloned canine AIM cDNA and observed its transcriptional expression levels in various tissues. The coding sequence of canine AIM was 1,023 bp encoding 340 amino acid residues, which had around 65% homology with those of the human, mouse and rat. Transcriptional expression of AIM was observed in the spleen, lung, liver and lymph node, which confirmed the expression of canine AIM in tissue macrophages. Moreover, AIM was highly expressed in one of the canine histiocytic sarcoma cell lines. CD36, the receptor of AIM, was also expressed in various tissues and these cell lines. These findings are useful to reveal the actual functions of canine AIM.
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spelling pubmed-43003822015-02-06 Molecular Cloning and Gene Expression of Canine Apoptosis Inhibitor of Macrophage TOMURA, Shintaro UCHIDA, Mona YONEZAWA, Tomohiro KOBAYASHI, Masato BONKOBARA, Makoto ARAI, Satoko MIYAZAKI, Toru TAMAHARA, Satoshi MATSUKI, Naoaki J Vet Med Sci Internal Medicine Apoptosis inhibitor of macrophage (AIM) plays roles in survival of macrophages. In this study, we cloned canine AIM cDNA and observed its transcriptional expression levels in various tissues. The coding sequence of canine AIM was 1,023 bp encoding 340 amino acid residues, which had around 65% homology with those of the human, mouse and rat. Transcriptional expression of AIM was observed in the spleen, lung, liver and lymph node, which confirmed the expression of canine AIM in tissue macrophages. Moreover, AIM was highly expressed in one of the canine histiocytic sarcoma cell lines. CD36, the receptor of AIM, was also expressed in various tissues and these cell lines. These findings are useful to reveal the actual functions of canine AIM. The Japanese Society of Veterinary Science 2014-09-01 2014-12 /pmc/articles/PMC4300382/ /pubmed/25649949 http://dx.doi.org/10.1292/jvms.14-0166 Text en ©2014 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Internal Medicine
TOMURA, Shintaro
UCHIDA, Mona
YONEZAWA, Tomohiro
KOBAYASHI, Masato
BONKOBARA, Makoto
ARAI, Satoko
MIYAZAKI, Toru
TAMAHARA, Satoshi
MATSUKI, Naoaki
Molecular Cloning and Gene Expression of Canine Apoptosis Inhibitor of Macrophage
title Molecular Cloning and Gene Expression of Canine Apoptosis Inhibitor of Macrophage
title_full Molecular Cloning and Gene Expression of Canine Apoptosis Inhibitor of Macrophage
title_fullStr Molecular Cloning and Gene Expression of Canine Apoptosis Inhibitor of Macrophage
title_full_unstemmed Molecular Cloning and Gene Expression of Canine Apoptosis Inhibitor of Macrophage
title_short Molecular Cloning and Gene Expression of Canine Apoptosis Inhibitor of Macrophage
title_sort molecular cloning and gene expression of canine apoptosis inhibitor of macrophage
topic Internal Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300382/
https://www.ncbi.nlm.nih.gov/pubmed/25649949
http://dx.doi.org/10.1292/jvms.14-0166
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