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Identification of novel viral receptors with cell line expressing viral receptor-binding protein
The viral cell receptors and infection can be blocked by the expression of the viral receptor-binding protein. Thus, the viral cell receptor is an attractive target for anti-viral strategies, and the identification of viral cell receptor is critical for better understanding and controlling viral dis...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300512/ https://www.ncbi.nlm.nih.gov/pubmed/25604889 http://dx.doi.org/10.1038/srep07935 |
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author | Mei, Mei Ye, Jianqiang Qin, Aijian Wang, Lin Hu, Xuming Qian, Kun Shao, Hongxia |
author_facet | Mei, Mei Ye, Jianqiang Qin, Aijian Wang, Lin Hu, Xuming Qian, Kun Shao, Hongxia |
author_sort | Mei, Mei |
collection | PubMed |
description | The viral cell receptors and infection can be blocked by the expression of the viral receptor-binding protein. Thus, the viral cell receptor is an attractive target for anti-viral strategies, and the identification of viral cell receptor is critical for better understanding and controlling viral disease. As a model system for viral entry and anti-retroviral approaches, avian sarcoma/leukosis virus (ASLV, including the A-J ten subgroups) has been studied intensively and many milestone discoveries have been achieved based on work with ASLV. Here, we used a DF1 cell line expressed viral receptor-binding protein to efficiently identify chicken Annexin A2 (chANXA2) as a novel receptor for retrovirus ALV-J (avian leukosis virus subgroup J). Our data demonstrate that antibodies or siRNA to chANXA2 significantly inhibited ALV-J infection and replication, and over-expression of chANXA2 permitted the entry of ALV-J into its non-permissible cells. Our findings have not only identified chANXA2 as a novel biomarker for anti-ALV-J, but also demonstrated that cell lines with the expression of viral receptor-binding protein could be as efficient tools for isolating functional receptors to identify novel anti-viral targets. |
format | Online Article Text |
id | pubmed-4300512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-43005122015-01-27 Identification of novel viral receptors with cell line expressing viral receptor-binding protein Mei, Mei Ye, Jianqiang Qin, Aijian Wang, Lin Hu, Xuming Qian, Kun Shao, Hongxia Sci Rep Article The viral cell receptors and infection can be blocked by the expression of the viral receptor-binding protein. Thus, the viral cell receptor is an attractive target for anti-viral strategies, and the identification of viral cell receptor is critical for better understanding and controlling viral disease. As a model system for viral entry and anti-retroviral approaches, avian sarcoma/leukosis virus (ASLV, including the A-J ten subgroups) has been studied intensively and many milestone discoveries have been achieved based on work with ASLV. Here, we used a DF1 cell line expressed viral receptor-binding protein to efficiently identify chicken Annexin A2 (chANXA2) as a novel receptor for retrovirus ALV-J (avian leukosis virus subgroup J). Our data demonstrate that antibodies or siRNA to chANXA2 significantly inhibited ALV-J infection and replication, and over-expression of chANXA2 permitted the entry of ALV-J into its non-permissible cells. Our findings have not only identified chANXA2 as a novel biomarker for anti-ALV-J, but also demonstrated that cell lines with the expression of viral receptor-binding protein could be as efficient tools for isolating functional receptors to identify novel anti-viral targets. Nature Publishing Group 2015-01-21 /pmc/articles/PMC4300512/ /pubmed/25604889 http://dx.doi.org/10.1038/srep07935 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Mei, Mei Ye, Jianqiang Qin, Aijian Wang, Lin Hu, Xuming Qian, Kun Shao, Hongxia Identification of novel viral receptors with cell line expressing viral receptor-binding protein |
title | Identification of novel viral receptors with cell line expressing viral receptor-binding protein |
title_full | Identification of novel viral receptors with cell line expressing viral receptor-binding protein |
title_fullStr | Identification of novel viral receptors with cell line expressing viral receptor-binding protein |
title_full_unstemmed | Identification of novel viral receptors with cell line expressing viral receptor-binding protein |
title_short | Identification of novel viral receptors with cell line expressing viral receptor-binding protein |
title_sort | identification of novel viral receptors with cell line expressing viral receptor-binding protein |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300512/ https://www.ncbi.nlm.nih.gov/pubmed/25604889 http://dx.doi.org/10.1038/srep07935 |
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