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CagA and VacA genotypes in peptic ulcer disease and non-ulcer dyspepsia: a case-control study

Background: The cag pathogenicity island includes a number of genes, including cytotoxin-associated protein A (cagA) and vacuolating cytotoxin (vacA) genotypes, which are associated with bacterial virulence. Although the role of cagA and vacA in the virulence of Helicobacter pylori (H. pylori) is we...

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Autores principales: FakhreYaseri, Hashem, Shakaraby, Mehdi, Bradaran, Hamid Reza, Soltani Arabshahi, Seyed Kamran, Fakhre Yaseri, Ali Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iran University of Medical Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4301206/
https://www.ncbi.nlm.nih.gov/pubmed/25664305
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author FakhreYaseri, Hashem
Shakaraby, Mehdi
Bradaran, Hamid Reza
Soltani Arabshahi, Seyed Kamran
Fakhre Yaseri, Ali Mohammad
author_facet FakhreYaseri, Hashem
Shakaraby, Mehdi
Bradaran, Hamid Reza
Soltani Arabshahi, Seyed Kamran
Fakhre Yaseri, Ali Mohammad
author_sort FakhreYaseri, Hashem
collection PubMed
description Background: The cag pathogenicity island includes a number of genes, including cytotoxin-associated protein A (cagA) and vacuolating cytotoxin (vacA) genotypes, which are associated with bacterial virulence. Although the role of cagA and vacA in the virulence of Helicobacter pylori (H. pylori) is well-established in epidemiological studies, the relationship between the cagA and vacA genotypes in Iran has yet to be fully elucidated. This study compared the association between cagA and vacA genotypes between peptic ulcer disease (PUD) patients and non-ulcer dyspeptic (NUD) patients. Methods: This case control study was done on 130 patients with positive H. pylori in histological and Giemsa reports. The case group comprised 65 PUD patients, and the control group included 65 NUD patients. The presence of the cagA and vacA genotypes was determined using polymerase chain reaction (PCR) on biopsy samples, taken via endoscopy. Results: Both cagA and vacA genotypes were positive in 51.5% (17) of the PUD group and 20% (6) of the NUD group (p= 0.009), and both cagA and vacA genotypes were negative in 48.5% (16) and 80% (24) of the case and control groups, respectively (p= 0.03). CagA-positive H. pylori was detected in 41.5% (27) and 24.6% (16) of the case and control groups, respectively (p= 0.001), and vacA-positive H. pylori was found in 60% (39) and 46% (30) of the case and control groups, respectively. Conclusion: Both cagA and vacA genotypes were more prevalent in the PUD patients than in their NUD counterparts among our Iranian samples. It is seems that the determination of these two genotypes in PUD patients is a good screening tool for patient selection for endoscopy and treatment.
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spelling pubmed-43012062015-02-06 CagA and VacA genotypes in peptic ulcer disease and non-ulcer dyspepsia: a case-control study FakhreYaseri, Hashem Shakaraby, Mehdi Bradaran, Hamid Reza Soltani Arabshahi, Seyed Kamran Fakhre Yaseri, Ali Mohammad Med J Islam Repub Iran Original Article Background: The cag pathogenicity island includes a number of genes, including cytotoxin-associated protein A (cagA) and vacuolating cytotoxin (vacA) genotypes, which are associated with bacterial virulence. Although the role of cagA and vacA in the virulence of Helicobacter pylori (H. pylori) is well-established in epidemiological studies, the relationship between the cagA and vacA genotypes in Iran has yet to be fully elucidated. This study compared the association between cagA and vacA genotypes between peptic ulcer disease (PUD) patients and non-ulcer dyspeptic (NUD) patients. Methods: This case control study was done on 130 patients with positive H. pylori in histological and Giemsa reports. The case group comprised 65 PUD patients, and the control group included 65 NUD patients. The presence of the cagA and vacA genotypes was determined using polymerase chain reaction (PCR) on biopsy samples, taken via endoscopy. Results: Both cagA and vacA genotypes were positive in 51.5% (17) of the PUD group and 20% (6) of the NUD group (p= 0.009), and both cagA and vacA genotypes were negative in 48.5% (16) and 80% (24) of the case and control groups, respectively (p= 0.03). CagA-positive H. pylori was detected in 41.5% (27) and 24.6% (16) of the case and control groups, respectively (p= 0.001), and vacA-positive H. pylori was found in 60% (39) and 46% (30) of the case and control groups, respectively. Conclusion: Both cagA and vacA genotypes were more prevalent in the PUD patients than in their NUD counterparts among our Iranian samples. It is seems that the determination of these two genotypes in PUD patients is a good screening tool for patient selection for endoscopy and treatment. Iran University of Medical Sciences 2014-09-28 /pmc/articles/PMC4301206/ /pubmed/25664305 Text en © 2014 Iran University of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial 3.0 License (CC BY-NC 3.0), which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
FakhreYaseri, Hashem
Shakaraby, Mehdi
Bradaran, Hamid Reza
Soltani Arabshahi, Seyed Kamran
Fakhre Yaseri, Ali Mohammad
CagA and VacA genotypes in peptic ulcer disease and non-ulcer dyspepsia: a case-control study
title CagA and VacA genotypes in peptic ulcer disease and non-ulcer dyspepsia: a case-control study
title_full CagA and VacA genotypes in peptic ulcer disease and non-ulcer dyspepsia: a case-control study
title_fullStr CagA and VacA genotypes in peptic ulcer disease and non-ulcer dyspepsia: a case-control study
title_full_unstemmed CagA and VacA genotypes in peptic ulcer disease and non-ulcer dyspepsia: a case-control study
title_short CagA and VacA genotypes in peptic ulcer disease and non-ulcer dyspepsia: a case-control study
title_sort caga and vaca genotypes in peptic ulcer disease and non-ulcer dyspepsia: a case-control study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4301206/
https://www.ncbi.nlm.nih.gov/pubmed/25664305
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