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A systematic review of physiological methods in rodent pharmacological MRI studies
RATIONALE: Pharmacological magnetic resonance imaging (phMRI) provides an approach to study effects of drug challenges on brain processes. Elucidating mechanisms of drug action helps us to better understand the workings of neurotransmitter systems, map brain function or facilitate drug development....
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4302233/ https://www.ncbi.nlm.nih.gov/pubmed/25585682 http://dx.doi.org/10.1007/s00213-014-3855-0 |
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author | Haensel, Jennifer X. Spain, Aisling Martin, Chris |
author_facet | Haensel, Jennifer X. Spain, Aisling Martin, Chris |
author_sort | Haensel, Jennifer X. |
collection | PubMed |
description | RATIONALE: Pharmacological magnetic resonance imaging (phMRI) provides an approach to study effects of drug challenges on brain processes. Elucidating mechanisms of drug action helps us to better understand the workings of neurotransmitter systems, map brain function or facilitate drug development. phMRI is increasingly used in preclinical research employing rodent models; however, data interpretation and integration are complicated by the use of different experimental approaches between laboratories. In particular, the effects of different anaesthetic regimes upon neuronal and haemodynamic processes and baseline physiology could be problematic. OBJECTIVES: This paper investigates how differences in phMRI research methodologies are manifested and considers associated implications, placing particular emphasis on choice of anaesthetic regimes. METHODS: A systematic review of rodent phMRI studies was conducted. Factors such as those describing anaesthetic regimes (e.g. agent, dosage) and parameters relating to physiological maintenance (e.g. ventilatory gases) and MRI method were recorded. RESULTS: We identified 126 eligible studies and found that the volatile agents isoflurane (43.7 %) and halothane (33.3 %) were most commonly used for anaesthesia, but dosage and mixture of ventilatory gases varied substantially between laboratories. Relevant physiological parameters were usually recorded, although 32 % of studies did not provide cardiovascular measures. CONCLUSIONS: Anaesthesia and animal preparation can influence phMRI data profoundly. The variation of anaesthetic type, dosage regime and ventilatory gases makes consolidation of research findings (e.g. within a specific neurotransmitter system) difficult. Standardisation of a small(er) number of preclinical phMRI research methodologies and/or increased consideration of approaches that do not require anaesthesia is necessary to address these challenges. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00213-014-3855-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4302233 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-43022332015-01-26 A systematic review of physiological methods in rodent pharmacological MRI studies Haensel, Jennifer X. Spain, Aisling Martin, Chris Psychopharmacology (Berl) Review RATIONALE: Pharmacological magnetic resonance imaging (phMRI) provides an approach to study effects of drug challenges on brain processes. Elucidating mechanisms of drug action helps us to better understand the workings of neurotransmitter systems, map brain function or facilitate drug development. phMRI is increasingly used in preclinical research employing rodent models; however, data interpretation and integration are complicated by the use of different experimental approaches between laboratories. In particular, the effects of different anaesthetic regimes upon neuronal and haemodynamic processes and baseline physiology could be problematic. OBJECTIVES: This paper investigates how differences in phMRI research methodologies are manifested and considers associated implications, placing particular emphasis on choice of anaesthetic regimes. METHODS: A systematic review of rodent phMRI studies was conducted. Factors such as those describing anaesthetic regimes (e.g. agent, dosage) and parameters relating to physiological maintenance (e.g. ventilatory gases) and MRI method were recorded. RESULTS: We identified 126 eligible studies and found that the volatile agents isoflurane (43.7 %) and halothane (33.3 %) were most commonly used for anaesthesia, but dosage and mixture of ventilatory gases varied substantially between laboratories. Relevant physiological parameters were usually recorded, although 32 % of studies did not provide cardiovascular measures. CONCLUSIONS: Anaesthesia and animal preparation can influence phMRI data profoundly. The variation of anaesthetic type, dosage regime and ventilatory gases makes consolidation of research findings (e.g. within a specific neurotransmitter system) difficult. Standardisation of a small(er) number of preclinical phMRI research methodologies and/or increased consideration of approaches that do not require anaesthesia is necessary to address these challenges. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00213-014-3855-0) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2015-01-15 2015 /pmc/articles/PMC4302233/ /pubmed/25585682 http://dx.doi.org/10.1007/s00213-014-3855-0 Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Review Haensel, Jennifer X. Spain, Aisling Martin, Chris A systematic review of physiological methods in rodent pharmacological MRI studies |
title | A systematic review of physiological methods in rodent pharmacological MRI studies |
title_full | A systematic review of physiological methods in rodent pharmacological MRI studies |
title_fullStr | A systematic review of physiological methods in rodent pharmacological MRI studies |
title_full_unstemmed | A systematic review of physiological methods in rodent pharmacological MRI studies |
title_short | A systematic review of physiological methods in rodent pharmacological MRI studies |
title_sort | systematic review of physiological methods in rodent pharmacological mri studies |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4302233/ https://www.ncbi.nlm.nih.gov/pubmed/25585682 http://dx.doi.org/10.1007/s00213-014-3855-0 |
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