Cargando…

A systematic review of physiological methods in rodent pharmacological MRI studies

RATIONALE: Pharmacological magnetic resonance imaging (phMRI) provides an approach to study effects of drug challenges on brain processes. Elucidating mechanisms of drug action helps us to better understand the workings of neurotransmitter systems, map brain function or facilitate drug development....

Descripción completa

Detalles Bibliográficos
Autores principales: Haensel, Jennifer X., Spain, Aisling, Martin, Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4302233/
https://www.ncbi.nlm.nih.gov/pubmed/25585682
http://dx.doi.org/10.1007/s00213-014-3855-0
_version_ 1782353759395905536
author Haensel, Jennifer X.
Spain, Aisling
Martin, Chris
author_facet Haensel, Jennifer X.
Spain, Aisling
Martin, Chris
author_sort Haensel, Jennifer X.
collection PubMed
description RATIONALE: Pharmacological magnetic resonance imaging (phMRI) provides an approach to study effects of drug challenges on brain processes. Elucidating mechanisms of drug action helps us to better understand the workings of neurotransmitter systems, map brain function or facilitate drug development. phMRI is increasingly used in preclinical research employing rodent models; however, data interpretation and integration are complicated by the use of different experimental approaches between laboratories. In particular, the effects of different anaesthetic regimes upon neuronal and haemodynamic processes and baseline physiology could be problematic. OBJECTIVES: This paper investigates how differences in phMRI research methodologies are manifested and considers associated implications, placing particular emphasis on choice of anaesthetic regimes. METHODS: A systematic review of rodent phMRI studies was conducted. Factors such as those describing anaesthetic regimes (e.g. agent, dosage) and parameters relating to physiological maintenance (e.g. ventilatory gases) and MRI method were recorded. RESULTS: We identified 126 eligible studies and found that the volatile agents isoflurane (43.7 %) and halothane (33.3 %) were most commonly used for anaesthesia, but dosage and mixture of ventilatory gases varied substantially between laboratories. Relevant physiological parameters were usually recorded, although 32 % of studies did not provide cardiovascular measures. CONCLUSIONS: Anaesthesia and animal preparation can influence phMRI data profoundly. The variation of anaesthetic type, dosage regime and ventilatory gases makes consolidation of research findings (e.g. within a specific neurotransmitter system) difficult. Standardisation of a small(er) number of preclinical phMRI research methodologies and/or increased consideration of approaches that do not require anaesthesia is necessary to address these challenges. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00213-014-3855-0) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4302233
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-43022332015-01-26 A systematic review of physiological methods in rodent pharmacological MRI studies Haensel, Jennifer X. Spain, Aisling Martin, Chris Psychopharmacology (Berl) Review RATIONALE: Pharmacological magnetic resonance imaging (phMRI) provides an approach to study effects of drug challenges on brain processes. Elucidating mechanisms of drug action helps us to better understand the workings of neurotransmitter systems, map brain function or facilitate drug development. phMRI is increasingly used in preclinical research employing rodent models; however, data interpretation and integration are complicated by the use of different experimental approaches between laboratories. In particular, the effects of different anaesthetic regimes upon neuronal and haemodynamic processes and baseline physiology could be problematic. OBJECTIVES: This paper investigates how differences in phMRI research methodologies are manifested and considers associated implications, placing particular emphasis on choice of anaesthetic regimes. METHODS: A systematic review of rodent phMRI studies was conducted. Factors such as those describing anaesthetic regimes (e.g. agent, dosage) and parameters relating to physiological maintenance (e.g. ventilatory gases) and MRI method were recorded. RESULTS: We identified 126 eligible studies and found that the volatile agents isoflurane (43.7 %) and halothane (33.3 %) were most commonly used for anaesthesia, but dosage and mixture of ventilatory gases varied substantially between laboratories. Relevant physiological parameters were usually recorded, although 32 % of studies did not provide cardiovascular measures. CONCLUSIONS: Anaesthesia and animal preparation can influence phMRI data profoundly. The variation of anaesthetic type, dosage regime and ventilatory gases makes consolidation of research findings (e.g. within a specific neurotransmitter system) difficult. Standardisation of a small(er) number of preclinical phMRI research methodologies and/or increased consideration of approaches that do not require anaesthesia is necessary to address these challenges. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00213-014-3855-0) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2015-01-15 2015 /pmc/articles/PMC4302233/ /pubmed/25585682 http://dx.doi.org/10.1007/s00213-014-3855-0 Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Review
Haensel, Jennifer X.
Spain, Aisling
Martin, Chris
A systematic review of physiological methods in rodent pharmacological MRI studies
title A systematic review of physiological methods in rodent pharmacological MRI studies
title_full A systematic review of physiological methods in rodent pharmacological MRI studies
title_fullStr A systematic review of physiological methods in rodent pharmacological MRI studies
title_full_unstemmed A systematic review of physiological methods in rodent pharmacological MRI studies
title_short A systematic review of physiological methods in rodent pharmacological MRI studies
title_sort systematic review of physiological methods in rodent pharmacological mri studies
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4302233/
https://www.ncbi.nlm.nih.gov/pubmed/25585682
http://dx.doi.org/10.1007/s00213-014-3855-0
work_keys_str_mv AT haenseljenniferx asystematicreviewofphysiologicalmethodsinrodentpharmacologicalmristudies
AT spainaisling asystematicreviewofphysiologicalmethodsinrodentpharmacologicalmristudies
AT martinchris asystematicreviewofphysiologicalmethodsinrodentpharmacologicalmristudies
AT haenseljenniferx systematicreviewofphysiologicalmethodsinrodentpharmacologicalmristudies
AT spainaisling systematicreviewofphysiologicalmethodsinrodentpharmacologicalmristudies
AT martinchris systematicreviewofphysiologicalmethodsinrodentpharmacologicalmristudies