Cargando…
An integrated phylogenomic approach toward pinpointing the origin of mitochondria
Overwhelming evidence supports the endosymbiosis theory that mitochondria originated once from the Alphaproteobacteria. However, its exact position in the tree of life remains highly debated. This is because systematic errors, including biased taxonomic sampling, high evolutionary rates and sequence...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4302308/ https://www.ncbi.nlm.nih.gov/pubmed/25609566 http://dx.doi.org/10.1038/srep07949 |
_version_ | 1782353774739718144 |
---|---|
author | Wang, Zhang Wu, Martin |
author_facet | Wang, Zhang Wu, Martin |
author_sort | Wang, Zhang |
collection | PubMed |
description | Overwhelming evidence supports the endosymbiosis theory that mitochondria originated once from the Alphaproteobacteria. However, its exact position in the tree of life remains highly debated. This is because systematic errors, including biased taxonomic sampling, high evolutionary rates and sequence composition bias have long plagued the mitochondrial phylogenetics. In this study, we address this issue by 1) increasing the taxonomic representation of alphaproteobacterial genomes by sequencing 18 phylogenetically novel species. They include 5 Rickettsiales and 4 Rhodospirillales, two orders that have shown close affiliations with mitochondria previously, 2) using a set of 29 slowly evolving mitochondria-derived nuclear genes that are less biased than mitochondria-encoded genes as the alternative “well behaved” markers for phylogenetic analysis, 3) applying site heterogeneous mixture models that account for the sequence composition bias. With the integrated phylogenomic approach, we are able to for the first time place mitochondria unequivocally within the Rickettsiales order, as a sister clade to the Rickettsiaceae and Anaplasmataceae families, all subtended by the Holosporaceae family. Our results suggest that mitochondria most likely originated from a Rickettsiales endosymbiont already residing in the host, but not from the distantly related free-living Pelagibacter and Rhodospirillales. |
format | Online Article Text |
id | pubmed-4302308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-43023082015-01-27 An integrated phylogenomic approach toward pinpointing the origin of mitochondria Wang, Zhang Wu, Martin Sci Rep Article Overwhelming evidence supports the endosymbiosis theory that mitochondria originated once from the Alphaproteobacteria. However, its exact position in the tree of life remains highly debated. This is because systematic errors, including biased taxonomic sampling, high evolutionary rates and sequence composition bias have long plagued the mitochondrial phylogenetics. In this study, we address this issue by 1) increasing the taxonomic representation of alphaproteobacterial genomes by sequencing 18 phylogenetically novel species. They include 5 Rickettsiales and 4 Rhodospirillales, two orders that have shown close affiliations with mitochondria previously, 2) using a set of 29 slowly evolving mitochondria-derived nuclear genes that are less biased than mitochondria-encoded genes as the alternative “well behaved” markers for phylogenetic analysis, 3) applying site heterogeneous mixture models that account for the sequence composition bias. With the integrated phylogenomic approach, we are able to for the first time place mitochondria unequivocally within the Rickettsiales order, as a sister clade to the Rickettsiaceae and Anaplasmataceae families, all subtended by the Holosporaceae family. Our results suggest that mitochondria most likely originated from a Rickettsiales endosymbiont already residing in the host, but not from the distantly related free-living Pelagibacter and Rhodospirillales. Nature Publishing Group 2015-01-22 /pmc/articles/PMC4302308/ /pubmed/25609566 http://dx.doi.org/10.1038/srep07949 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ |
spellingShingle | Article Wang, Zhang Wu, Martin An integrated phylogenomic approach toward pinpointing the origin of mitochondria |
title | An integrated phylogenomic approach toward pinpointing the origin of mitochondria |
title_full | An integrated phylogenomic approach toward pinpointing the origin of mitochondria |
title_fullStr | An integrated phylogenomic approach toward pinpointing the origin of mitochondria |
title_full_unstemmed | An integrated phylogenomic approach toward pinpointing the origin of mitochondria |
title_short | An integrated phylogenomic approach toward pinpointing the origin of mitochondria |
title_sort | integrated phylogenomic approach toward pinpointing the origin of mitochondria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4302308/ https://www.ncbi.nlm.nih.gov/pubmed/25609566 http://dx.doi.org/10.1038/srep07949 |
work_keys_str_mv | AT wangzhang anintegratedphylogenomicapproachtowardpinpointingtheoriginofmitochondria AT wumartin anintegratedphylogenomicapproachtowardpinpointingtheoriginofmitochondria AT wangzhang integratedphylogenomicapproachtowardpinpointingtheoriginofmitochondria AT wumartin integratedphylogenomicapproachtowardpinpointingtheoriginofmitochondria |