Comparison of nested, multiplex, qPCR; FISH; SeptiFast and blood culture methods in detection and identification of bacteria and fungi in blood of patients with sepsis

BACKGROUND: Microbiological diagnosis of sepsis relies primarily on blood culture data. This study compares four diagnostic methods, i.e. those developed by us: nested, multiplex, qPCR (qPCR) and FISH with commercial methods: SeptiFast (Roche) (SF) and BacT/ALERT® 3D blood culture system (bioMérieux...

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Detalles Bibliográficos
Autores principales: Gosiewski, Tomasz, Flis, Agnieszka, Sroka, Agnieszka, Kędzierska, Anna, Pietrzyk, Agata, Kędzierska, Jolanta, Drwiła, Rafał, Bulanda, Małgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4302608/
https://www.ncbi.nlm.nih.gov/pubmed/25551203
http://dx.doi.org/10.1186/s12866-014-0313-4

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