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Non-invasive imaging of allogeneic transplanted skin graft by (131)I-anti-TLR5 mAb

Although (18)F-fluorodeoxyglucose ((18)F-FDG) uptake can be used for the non-invasive detection and monitoring of allograft rejection by activated leucocytes, this non-specific accumulation is easily impaired by immunosuppressants. Our aim was to evaluate a (131)I-radiolabelled anti-Toll-like recept...

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Autores principales: Sun, Hukui, Yang, Guangjie, Liang, Ting, Zhang, Chao, Song, Jing, Han, Jiankui, Hou, Guihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4302649/
https://www.ncbi.nlm.nih.gov/pubmed/25283154
http://dx.doi.org/10.1111/jcmm.12423
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author Sun, Hukui
Yang, Guangjie
Liang, Ting
Zhang, Chao
Song, Jing
Han, Jiankui
Hou, Guihua
author_facet Sun, Hukui
Yang, Guangjie
Liang, Ting
Zhang, Chao
Song, Jing
Han, Jiankui
Hou, Guihua
author_sort Sun, Hukui
collection PubMed
description Although (18)F-fluorodeoxyglucose ((18)F-FDG) uptake can be used for the non-invasive detection and monitoring of allograft rejection by activated leucocytes, this non-specific accumulation is easily impaired by immunosuppressants. Our aim was to evaluate a (131)I-radiolabelled anti-Toll-like receptor 5 (TLR5) mAb for non-invasive in vivo graft visualization and quantification in allogeneic transplantation mice model, compared with the non-specific radiotracer (18)F-FDG under using of immunosuppressant. Labelling, binding, and stability studies were performed. BALB/c mice transplanted with C57BL/6 skin grafts, with or without rapamycin treatment (named as allo-treated group or allo-rejection group), were injected with (131)I-anti-TLR5 mAb, (18)F-FDG, or mouse isotype (131)I-IgG, respectively. Whole-body phosphor-autoradiography and ex vivo biodistribution studies were obtained. Whole-body phosphor-autoradiography showed (131)I-anti-TLR5 mAb uptake into organs that were well perfused with blood at 1 hr and showed clear graft images from 12 hrs onwards. The (131)I-anti-TLR5 mAb had significantly higher graft uptake and target-to-non-target ratio in the allo-treated group, as determined by semi-quantification of phosphor-autoradiography images; these results were consistent with ex vivo biodistribution studies. However, high (18)F-FDG uptake was not observed in the allo-treated group. The highest allograft-skin-to-native-skin ratio (A:N) of (131)I-anti-TLR5 mAb uptake was significantly higher than the ratio for (18)F-FDG (7.68 versus 1.16, respectively). (131)I-anti-TLR5 mAb uptake in the grafts significantly correlated with TLR5 expression in the allograft area. The accumulation of (131)I-IgG was comparable in both groups. We conclude that radiolabelled anti-TLR5 mAb is capable of detecting allograft with high target specificity after treatment with the immunosuppressive drug rapamycin.
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spelling pubmed-43026492015-01-22 Non-invasive imaging of allogeneic transplanted skin graft by (131)I-anti-TLR5 mAb Sun, Hukui Yang, Guangjie Liang, Ting Zhang, Chao Song, Jing Han, Jiankui Hou, Guihua J Cell Mol Med Original Articles Although (18)F-fluorodeoxyglucose ((18)F-FDG) uptake can be used for the non-invasive detection and monitoring of allograft rejection by activated leucocytes, this non-specific accumulation is easily impaired by immunosuppressants. Our aim was to evaluate a (131)I-radiolabelled anti-Toll-like receptor 5 (TLR5) mAb for non-invasive in vivo graft visualization and quantification in allogeneic transplantation mice model, compared with the non-specific radiotracer (18)F-FDG under using of immunosuppressant. Labelling, binding, and stability studies were performed. BALB/c mice transplanted with C57BL/6 skin grafts, with or without rapamycin treatment (named as allo-treated group or allo-rejection group), were injected with (131)I-anti-TLR5 mAb, (18)F-FDG, or mouse isotype (131)I-IgG, respectively. Whole-body phosphor-autoradiography and ex vivo biodistribution studies were obtained. Whole-body phosphor-autoradiography showed (131)I-anti-TLR5 mAb uptake into organs that were well perfused with blood at 1 hr and showed clear graft images from 12 hrs onwards. The (131)I-anti-TLR5 mAb had significantly higher graft uptake and target-to-non-target ratio in the allo-treated group, as determined by semi-quantification of phosphor-autoradiography images; these results were consistent with ex vivo biodistribution studies. However, high (18)F-FDG uptake was not observed in the allo-treated group. The highest allograft-skin-to-native-skin ratio (A:N) of (131)I-anti-TLR5 mAb uptake was significantly higher than the ratio for (18)F-FDG (7.68 versus 1.16, respectively). (131)I-anti-TLR5 mAb uptake in the grafts significantly correlated with TLR5 expression in the allograft area. The accumulation of (131)I-IgG was comparable in both groups. We conclude that radiolabelled anti-TLR5 mAb is capable of detecting allograft with high target specificity after treatment with the immunosuppressive drug rapamycin. Blackwell Publishing Ltd 2014-12 2014-10-06 /pmc/articles/PMC4302649/ /pubmed/25283154 http://dx.doi.org/10.1111/jcmm.12423 Text en © 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Sun, Hukui
Yang, Guangjie
Liang, Ting
Zhang, Chao
Song, Jing
Han, Jiankui
Hou, Guihua
Non-invasive imaging of allogeneic transplanted skin graft by (131)I-anti-TLR5 mAb
title Non-invasive imaging of allogeneic transplanted skin graft by (131)I-anti-TLR5 mAb
title_full Non-invasive imaging of allogeneic transplanted skin graft by (131)I-anti-TLR5 mAb
title_fullStr Non-invasive imaging of allogeneic transplanted skin graft by (131)I-anti-TLR5 mAb
title_full_unstemmed Non-invasive imaging of allogeneic transplanted skin graft by (131)I-anti-TLR5 mAb
title_short Non-invasive imaging of allogeneic transplanted skin graft by (131)I-anti-TLR5 mAb
title_sort non-invasive imaging of allogeneic transplanted skin graft by (131)i-anti-tlr5 mab
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4302649/
https://www.ncbi.nlm.nih.gov/pubmed/25283154
http://dx.doi.org/10.1111/jcmm.12423
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